One reason glutamate rises with methylfolate is by conversion of histidine to a form of glutamate before it can become histamine. This is a classic sign of over methylation (really low histamine, overly high glutamate). There are of course other reasons glutamate can rise to unacceptable levels (i.e. low levels of glutamic acid decarboxylase, low levels of glutamine synthetase, dietary intake, Cystathione beta mutation, etc.). In my case I have Stiff Person Syndrome, which means my body attacks my GAD enzymes in my CNS and I have to control it with corticosteroids, hence I always have a baseline of neurological inflammation I can never exterminate entirely. To reduce glutamate levels also expunge all gluten from your diet. That is more important imo than eliminating glutamine provided it is at low levels for intestinal and muscle health (your body makes 100s of grams of glutamine on its own anyways otherwise you would waste away in no time).