vortex
Senior Member
- Messages
- 162
I would like to put for the theory that peroxynitrite is a significant obstacle to
healing via methylation protocol in ways we havent realized by interfering
and or destroying much of our effort by the methylation protocol and some
degree of success in the methylation protocol comes from an inadvertent
co-treating and overcoming peroxynitrite.
In other words, peroxynitrite is contributing to keeping the mitochondria
shut down in more ways than is attributed in the current theory.
richvank theory mentions peroxynitrite inhibits methylfolate in #3 but leaves
out some important details of its real impact in other areas downstream.
http://forums.phoenixrising.me/inde...tion-cycle-block-hypothesis-for-me-cfs.15701/
Yes, peroxynitrite inhibits methylfolate but it also has its hand in shutting down other cycles
in the methylation cycle and glutathione cycle.
Peroxynitrite directly induces destruction of the tetrahydrobiopterin
http://www.ncbi.nlm.nih.gov/pubmed/20184376
Peroxynitrite Inactivation of Glutathione Peroxidase
http://www.sciencedirect.com/science/article/pii/S0003986197904070
But in #4 Rich attributes "Glutathione depletion lowers the affinity of the CblC"
as the start of the block/cause, theorizing this is shutting down "methionine synthase reaction" causing the block and focusing on this can fix things.
Although he then goes back to and mentions peroxynitrite in #7
"The elevated peroxynitrite catabolizes methylfolate, preventing its rise in the plasma"
he mentions it inhibits methylfolate which could contribute to some of this block but
then stops there and goes back to b-12. It almost seems like the peroxynitrite is incorporated
into this theory by mentioning it and saying its impact is that it inhibits methylfolate but then
stops short there.
He then goes back to b12 in #13 by saying the treatment should revolve
around the b-12
"dosage of B12 is necessary to compensate for the greatly lowered affinity for cobalamin of the CblC complementation group, so as to overcome the functional B12 deficiency,"
So if peroxynitrite can inhibit glutathione recycling and destruction of BH4 in addition to destruction of methylfolate which inhibits methionine synthase, who knows, this might be the real reason glutathione doesnt come back up and causes CFS instead of b-12. I think peroxynitrite is interfering with more steps than we realize and that by treating this with methylation boosters like methylfolate and methylcobalamin we arent just restoring methionine synthase reactions, but we may be treating peroxynitrite directly with the supplements we are using or indirectly by overdriving methionine synthase to compensate for peroxynitrite effects of destroying bh4 and glutathione peroxidase.
hydroxocobalamin is a peroxnitrite scavenger, which may explain why people are having a good response to this cobal over methylcobalamin which should be the only one that significantly boosts methionine synthase.
Also methylfolate is a peroxnitrite scavenger, so it makes you wonder how much methylfolate is going towards quenching peroxynitrite and how much is going towards methionine synthase ?
So it seems like peroxynitrite is destroying alot of our efforts and is like stepping harder on the gas while having the brakes on or turning the faucet on faster to try to fill the sink because the drain is partially open.
So I think that if this is true, then we should focus more on directly addressing peroxynitrite/nitrotyrosine scavenging directly so we dont have to put all the load on the methionine synthase reaction to overcome all of the destruction that peroxynitrite is causing.
So perhaps adding more peroxynitrite scavengers to the methylation protocol can help non-responders and or alter or reduce some of the doses of the methylation boosters so we dont have to compensate so much for all the destruction that peroxynitrite is doing to our efforts.
gamma tocopherol is a known peroxynitrite scavenger, can someone add to this list ?
This document by pall discusses some strategies to lower peroxynitrite
http://www.csom.ca/wp-content/uploa...g-was-Right-but-for-the-Wrong-Reason-25.3.pdf
healing via methylation protocol in ways we havent realized by interfering
and or destroying much of our effort by the methylation protocol and some
degree of success in the methylation protocol comes from an inadvertent
co-treating and overcoming peroxynitrite.
In other words, peroxynitrite is contributing to keeping the mitochondria
shut down in more ways than is attributed in the current theory.
richvank theory mentions peroxynitrite inhibits methylfolate in #3 but leaves
out some important details of its real impact in other areas downstream.
http://forums.phoenixrising.me/inde...tion-cycle-block-hypothesis-for-me-cfs.15701/
Yes, peroxynitrite inhibits methylfolate but it also has its hand in shutting down other cycles
in the methylation cycle and glutathione cycle.
Peroxynitrite directly induces destruction of the tetrahydrobiopterin
http://www.ncbi.nlm.nih.gov/pubmed/20184376
Peroxynitrite Inactivation of Glutathione Peroxidase
http://www.sciencedirect.com/science/article/pii/S0003986197904070
But in #4 Rich attributes "Glutathione depletion lowers the affinity of the CblC"
as the start of the block/cause, theorizing this is shutting down "methionine synthase reaction" causing the block and focusing on this can fix things.
Although he then goes back to and mentions peroxynitrite in #7
"The elevated peroxynitrite catabolizes methylfolate, preventing its rise in the plasma"
he mentions it inhibits methylfolate which could contribute to some of this block but
then stops there and goes back to b-12. It almost seems like the peroxynitrite is incorporated
into this theory by mentioning it and saying its impact is that it inhibits methylfolate but then
stops short there.
He then goes back to b12 in #13 by saying the treatment should revolve
around the b-12
"dosage of B12 is necessary to compensate for the greatly lowered affinity for cobalamin of the CblC complementation group, so as to overcome the functional B12 deficiency,"
So if peroxynitrite can inhibit glutathione recycling and destruction of BH4 in addition to destruction of methylfolate which inhibits methionine synthase, who knows, this might be the real reason glutathione doesnt come back up and causes CFS instead of b-12. I think peroxynitrite is interfering with more steps than we realize and that by treating this with methylation boosters like methylfolate and methylcobalamin we arent just restoring methionine synthase reactions, but we may be treating peroxynitrite directly with the supplements we are using or indirectly by overdriving methionine synthase to compensate for peroxynitrite effects of destroying bh4 and glutathione peroxidase.
hydroxocobalamin is a peroxnitrite scavenger, which may explain why people are having a good response to this cobal over methylcobalamin which should be the only one that significantly boosts methionine synthase.
Also methylfolate is a peroxnitrite scavenger, so it makes you wonder how much methylfolate is going towards quenching peroxynitrite and how much is going towards methionine synthase ?
So it seems like peroxynitrite is destroying alot of our efforts and is like stepping harder on the gas while having the brakes on or turning the faucet on faster to try to fill the sink because the drain is partially open.
So I think that if this is true, then we should focus more on directly addressing peroxynitrite/nitrotyrosine scavenging directly so we dont have to put all the load on the methionine synthase reaction to overcome all of the destruction that peroxynitrite is causing.
So perhaps adding more peroxynitrite scavengers to the methylation protocol can help non-responders and or alter or reduce some of the doses of the methylation boosters so we dont have to compensate so much for all the destruction that peroxynitrite is doing to our efforts.
gamma tocopherol is a known peroxynitrite scavenger, can someone add to this list ?
This document by pall discusses some strategies to lower peroxynitrite
http://www.csom.ca/wp-content/uploa...g-was-Right-but-for-the-Wrong-Reason-25.3.pdf
Last edited: