Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties

nerd

Senior Member
Messages
863
Likes
2,451
Authors: Asif Halimi, Giorgio Gabarrini, Michał Jacek Sobkowiak, Zeeshan Ateeb, Haleh Davanian, Rogier Aäron Gaiser, Urban Arnelo, Roberto Valente, Alicia Y.W. Wong, Carlos Fernández Moro, Marco Del Chiaro, Volkan Özenci & Margaret Sällberg Chen
Published online: November 24, 2021
doi: 10.1080/19490976.2021.1983101

Abstract
Halimi et al. said:
Emerging research suggests gut microbiome may play a role in pancreatic cancer initiation and progression, but cultivation of the cancer microbiome remains challenging. This pilot study aims to investigate the possibility to cultivate pancreatic microbiome from pancreatic cystic lesions associated with invasive cancer. Intra-operatively acquired pancreatic cyst fluid samples showed culture-positivity mainly in the intraductal papillary mucinous neoplasm (IPMN) group of lesions. MALDI-TOF MS profiling analysis shows Gammaproteobacteria and Bacilli dominate among individual bacteria isolates. Among cultivated bacteria, Gammaproteobacteria, particularly Klebsiella pneumoniae, but also Granulicatella adiacens and Enterococcus faecalis, demonstrate consistent pathogenic properties in pancreatic cell lines tested in ex vivo co-culture models. Pathogenic properties include intracellular survival capability, cell death induction, or causing DNA double-strand breaks in the surviving cells resembling genotoxic effects. This study provides new insights into the role of the pancreatic microbiota in the intriguing link between pancreatic cystic lesions and cancer.
Halimi et al. said:
Specifically, S. maltophilia is known to cause nosocomial infections in the bloodstream, urinary and respiratory tract.
 

nerd

Senior Member
Messages
863
Likes
2,451
Here is a news article on it:

ScienceDaily: New findings on bacteria that increase risk of pancreatic cancer

Date: November 24, 2021
Link: https://www.sciencedaily.com/releases/2021/11/211124154110.htm

Summary
Bacteria from the digestive system seem to have the potential to cause damage to pancreatic cells, increasing the risk of malignant tumors. Now for the first time, live bacteria from cystic pancreatic lesions that are precursors to pancreatic cancer, have been analyzed by researchers. The study can lead to prophylactic interventions using local antibiotics.