@dbose L- Ornithine- I take 500-1000 daily, usually in the evening as it can have a very calming effect and falls within that period of the circadian rhythm very nicely but you could have other peripheral things going on that change your response so play around and see what works, might be 500 might be 1.5 grams, completely depends on you. Just don't do any arginine as you're likely to have BH4 involvement and peroxynitrates would be the last thing you need roaming around your body.
Protein Magnesium Threonate- This depends entirely on the type of meat you choose, the state of your urine strips, your response to meat in terms of PH, digestion and the inflow/outflow of sulfur to and from the body. Some people may need to completely get rid of meat until their toxicity comes way down (usually > or = 1,300-1,700 on urine sulfate strips) then add it back in later once the transsulfuration pathways burden is clear. At 800 I was able to consume 2-4 oz of meat but I chose dark meat turkey (given your COMT +/+ high tyrosine isn't recommended so maybe dark meat chicken would work) that has less methionine and is generally fattier. Correct, sulfur veggies contain high numbers of Thiol groups and that's the huge difference:
“You’ll find a lot of misleading ‘sulfur’ lists on the web. I’m talking about those compounds that are thiols or that metabolically convert to them, not to the total amount of elemental sulfur in the food. Some forms don’t convert very much and are tolerated at much higher levels than others. E.g. meat is in theory quite sulfurous, but in fact a lot of the sulfur stays in the methionine rather than thiol form so it is a less potent thiol source than an elemental analysis would suggest. Especially if you are taking TMG or choline which helps provide methyl groups so the methionine doesn’t need to get metabolized.” - Andrew Cutler
I'm not endorsing Andrew Cutler's protocol, I'm using this as a template because we may have high mercury as a consequence of our genetics but the root problem is sulfur and ammonia and more specifically high thiol containing (literally called mercaptans because they are also very efficient with capturing mercury) foods that are extremely efficient at accelerating the metabolism of sulfur because they are sulfur analogues of alcohols and a sulfhyrdryl group replaces the oxygen group. This means 2 things: 1. They break down very quickly and we know that CBS as an up regulation is already over functioning so that's why we experience symptoms nwhen we consume them. 2. they're actually more acidic for us (yes inside the body after being deprotonated if we can't metabolism them properly!) so if we have thiolates in high numbers bonded to mercury that would explain a lot of metabolic issues. (Those of you that suspect ANION Gap or Metabolic Acidosis and have severely increased CBS activity (high level toxicity) and correlated end product dysfunction, ATP/mtDNA issues will love what that the next part means).
This means also means that one of the biochemical end products of CBS (AKB NOT AKG!!!! Yasko is wrong here. Please spread this as that's a big thing among those of us that have ME/CFS because it changes the structure and direction of some programs entirely...) used as a very important aspect of mtDNA and ATP production is functioning in a sub-optimal fashion and potentially causing thyroid dysfunction, decreased energy production and chronic illness. AKB is one of the most important aspects of the mitochondrial matrix and krebs cycle so for those of us with Thyroid disfunction and under performing mtDNA related issues here's a potential answer along with IgA and IgE or TPH antibody involvement. (long side bar, this is why I suggested biotin, vitamin E succinate and adenosly/hydroxy b12 in my first post to you a long time ago, people probably thought I was nuts but now you'll see why, and this will answer your magnesium threonate question as well): AKB is the result of the lysis of
Cystathione and is one of the degradation products of threonine and the catabolism of the amino acid threonine dehydratase. It's also produced by the degradation of homocysteine and the metabolism of methionine. Here is the conversion from AKB => Succinyl CoA and entry to the Krebs Cycle
AKB, Alpha Keto Acid Dehydrogenase -> Proprionyl CoA, Proprionyl CoA Carboxylase +
biotin = (S) Methylmalonyl CoA => (R) Methylmalonly CoA, Methylmalonyl CoA Epemirase -> Methmalonyl CoA Mutase +
AdoCbl = Succinyl CoA ->
Krebs Cycle. This reaction helps generate functional platforms for new mtDNA and ATP synthesis. So treating any immune disturbances you have while nurturing this will greatly help you improve.
Magnesium Threonate should be just fine but I would take less than the recommended amount if you struggle and keep your magnesium citrate close. Slow moving COMT can keep SAMe around for too long and that can also cause a backup because you're not breaking down and cycling neurotransmitters quickly (thats a really nasty combination). I know I'm intervening in methionine metabolism by taking TMG and Choline (which I need because COMT -/- and VDR taq +/+ is low dopamine and greatest need for methyl donors) but for you, being the opposite, meat would would help in a very small manner of speaking but limit it and you might need to even more because you won't have the donors to block methionine metabolism and your COMT is already slow (Would love to know your VDR taq though, if you're +/+ or +/- that would be a little relief but if you're -/- I'm really sorry). 1-3 oz is OK for now, but mess around with the amount and see what you can tolerate. What you're titrating for is the point where you have enough for AKB, other reactions and nutritional maintenance without the 4-6g of ammonia on top of other metabolic waste left over because that will make things run MUCH slower and add toxicity via the other biochemical phenomena occurring within you. You can push and eventually liberalize your diet a little bit (that's relative to gut health keep in mind) once you drop your sulfur strip readings and your body repairs itself. I didn't add this before because you'd have hated me but NAD+ will induce microphagy if cells are properly supported and that will clear bad mtDNA and help you cycle SAMe potentially but it can make you feel pretty bad, kind of like a detox reaction. I posted small bit about this phenomenon earlier as well.
Turmeric/Curcumin is also very good at raising thiol groups but a little bit will help push your body towards making glutathione instead of taurine so there is that added benefit as well. I wouldn't shy away from using methyl donors just don't take a bath in them. You'll over do it and overdrive yourself quickly, especially with MTHF SNPs. MTHF enzymes are rate limiting for methylation generally so if you put the gas on and go wild with MTHF without the proper enzyme function you could put yourself in hyper drive because you've given yourself what you need but the body has no way of down regulating the over activity.