Unfolded Protein Response and A Possible Treatment for CFS

[girlinthesnow]

@mariovitali here are my results in bold:

GCH1
rs12147422 (Risk C) C;T
rs3783637 (Risk T) C;T
rs7147286 (Risk A) A;G
rs7492600 (Risk T) G;T
rs8004018 (Risk G) A;G
rs 3783642 (Risk C) C;T

ER Stress response

none of those listed

Choline Metabolism (impaired Choline absorption => impaired TMAO => ER Stress+UPR)

rs7946 PEMT (Risk T) T;T
rs4244593 PEMT (Risk G) G;T

CBS

rs2851391 (Risk T) C;T
rs706209 (Risk A) A;G
rs4920037 (Risk A) A;G
rs706208 (Risk G) A;G

MTHFR

rs2066470 (Risk A) A;A
rs4846049 (Risk T) T;T
rs1801131 (Risk G) G;G
rs 17367504 (Risk G) G;G
rs13306561 (Risk G) G;G
rs17037390 (Risk A) A;A
rs17037396 (Risk T) T;T
rs12121543 (Risk A) A;A
rs 1476413 (Risk T) T;T
rs1476413 (Risk T) C;T

NDUFS7 +/+
rs2332496
rs1142530
rs7258846
rs11666067
rs2074895

I've dozens more +/+, too many to list. Anything else that might be of use here?

Many thanks!

 

[mariovitali]

@mariovitali here are my results in bold:

GCH1
rs12147422 (Risk C) C;T
rs3783637 (Risk T) C;T
rs7147286 (Risk A) A;G
rs7492600 (Risk T) G;T
rs8004018 (Risk G) A;G
rs 3783642 (Risk C) C;T

ER Stress response

none of those listed

Choline Metabolism (impaired Choline absorption => impaired TMAO => ER Stress+UPR)

rs7946 PEMT (Risk T) T;T
rs4244593 PEMT (Risk G) G;T

CBS

rs2851391 (Risk T) C;T
rs706209 (Risk A) A;G
rs4920037 (Risk A) A;G
rs706208 (Risk G) A;G

MTHFR

rs2066470 (Risk A) A;A
rs4846049 (Risk T) T;T
rs1801131 (Risk G) G;G
rs 17367504 (Risk G) G;G
rs13306561 (Risk G) G;G
rs17037390 (Risk A) A;A
rs17037396 (Risk T) T;T
rs12121543 (Risk A) A;A
rs 1476413 (Risk T) T;T
rs1476413 (Risk T) C;T

NDUFS7 +/+
rs2332496
rs1142530
rs7258846
rs11666067
rs2074895

I've dozens more +/+, too many to list. Anything else that might be of use here?

Many thanks!

It is good that you do not have any problems in ER Stress response. Could you also share your regimen and symptoms?

 

[girlinthesnow]

It is good that you do not have any problems in ER Stress response. Could you also share your regimen and symptoms?

Thanks for your kind offer to look at this. I have great difficulty with organising my thoughts but will try.
Here goes...

Apart from childhood illnesses I was strong and well but have always had low BP, low body temperature and OI.

About 25 years my thyroid suddenly stopped functioning properly although I don't have Hashimotos.

Sudden allergy to latex and food reactions to grains, dairy, solanaceae and most common foods.
I eat a Paleo diet of 60% veg, the rest is meat, fish, fruit and oil (coconut & olive)

At the same time I developed anxiety after a short flu-like illness, literally from fine one day to paralysed with anxiety the next. Soon afterwards came muscle pain and lack of endurance.

After a couple of years brain symptoms appeared:
memory loss (I'd had a photographic memory and synasthesia, both of these vanished)
sudden anger and emotional lability
creative thinking diminished (I'd been a poet and written stories and articles)
a new difficulty with organising and planning

I have an 'allergic feeling in my head' which feels like sharp like pinpricks or the sensation of nettle rash but internally. I attempt to control this (high histamine?) with large doses of vitamin C, quercetin, basil and ginger.

I also get frequent migraines which become chronic, in a pattern of 3 days on, one day off, lasting up to 3 weeks.

Chronic intractable insomnia: asleep at 10pm, awake at 12am until 2 or 3 am, take L-carnosine and sleep until 5 or sometimes 7 am so an average of 5 hours sleep but one night in three I have no sleep.

These all persist along with chronic viral infections (EBV, Herpes simples, HZV) and also Bartonella so there is obvious immune insufficiency.

My regime is:

AM

3am to 5 am
B-alanyl-L-histidine 100 mg

on waking:
epsom bath (3 cups of Mg sulphate)
2 cups of fresh coffee
acyclovir 800 mg (twice a day)

1 hour later:
1.5 grains of Armour
Coq10 x 200mg x2/day

A-l-carnitine 250 mg (1x 2x/day)
B1 as thiamine HCL trace once day
Riboflavin 5 phosphate sodium 10mg/day
calcium d-pantothenate 50 mg/day
P5P trace
5-mthf 1mg
HB12 1 mg IM/week
choline bitartrate+inositol 25mg/day

L-lysine 500 mg (3x/day)
monolaurin 600 mg +inosine 7.5 mg
and/or humic monolaurin 500 mg + olive leaf ext(15% oleuropin 350 mg + humic acid 250 mg + L rhamnosus in lysate powder 25 mg (Either or both of these 3x/day)

Vitamin C 1 g 1x3x/day
quercetin dihydrate 500 mg + Vit C 1400 mg
zinc picolinate 22 mg one a day

Taken throughout the day:
Mg citrate 167 mg
Mg malate 200 mg
Mg glycinate 125 mg

Mid-morning to afternoon:
nascent iodine 400 mcg /day 1 drop applied to skin
EGCG 50%polyphenols green tea extract Jarrow
allicin max 180 mg allicin 1x2x/day
DHEA trace once a day just started last week titrating up
milk thistle 2000 mg 3 days a week

serrapeptase 120,000 SPU
nattokinase 146 mg/day

betaine HCL 650 mg + pepsin 165 mg (1x 2x/day)
digestive enzymes Source Naturals with each meal
Bifidobacterium infantis (align)
Biokult probiotic

I try to walk during the day, 20 to 30 minutes on flat ground, it improves mood but makes sleep worse.

Bedtime around 9:30 pm
Mg taurate 250 mg
herbal tea of linden flower, hops, lemon balm

 

[mariovitali]

Thanks for your kind offer to look at this. I have great difficulty with organising my thoughts but will try.
Here goes...

Apart from childhood illnesses I was strong and well but have always had low BP, low body temperature and OI.

About 25 years my thyroid suddenly stopped functioning properly although I don't have Hashimotos.

Sudden allergy to latex and severe food reactions to all grains, all dairy, solanaceae and most common foods
I eat a Paleo diet of 60% veg, the rest is meat, fish, fruit and oil (coconut & olive)

At the same time I developed anxiety after a short flu-like illness, literally from fine one day to paralysed with anxiety the next. Soon afterwards came muscle pain and lack of endurance.

After a couple of years brain symptoms appeared:
memory loss (I'd had a photographic memory and synasthesia, both of these vanished)
sudden anger and emotional lability
creative thinking diminished (I'd been a poet and written stories and articles)
a new difficulty with organising and planning

I have an 'allergic feeling in my head' which feels like sharp like pinpricks or the sensation of nettle rash but internally. I attempt to control this (high histamine?) with large doses of vitamin C, quercetin, basil and ginger.

I also get frequent migraines which become chronic, lasting up to 3 weeks each.

Chronic intractable insomnia: asleep at 10pm, awake at 12am until 2 or 3 am, take L-carnosine and sleep until 5 or sometimes 7 am so an average of 5 hours sleep but one night in three I have no sleep.

These all persist along with chronic viral infections (EBV, Herpes simples, HZV) and also Bartonella so there is obvious immune insufficiency.

My regime is:

AM

3am to 5 am
B-alanyl-L-histidine 100 mg

on waking:
epsom bath (3 cups of Mg sulphate)
2 cups of fresh coffee
acyclovir 800 mg (twice a day)

1 hour later:
1.5 grains of Armour
Coq10 x 200mg x2/day

A-l-carnitine 250 mg (1x 2x/day)
B1 as thiamine HCL trace once day
Riboflavin 5 phosphate sodium 10mg/day
calcium d-pantothenate 50 mg/day
P5P trace
5-mthf 1mg
HB12 1 mg IM/week
choline bitartrate+inositol 25mg/day

L-lysine 500 mg (3x/day)
monolaurin 600 mg +inosine 7.5 mg
and/or humic monolaurin 500 mg + olive leaf ext(15% oleuropin 350 mg + humic acid 250 mg + L rhamnosus in lysate powder 25 mg (Either or both of these 3x/day)

Vitamin C 1 g 1x3x/day
quercetin dihydrate 500 mg + Vit C 1400 mg
zinc picolinate 22 mg one a day

Taken throughout the day:
Mg citrate 167 mg
Mg malate 200 mg
Mg glycinate 125 mg

Mid-morning to afternoon:
nascent iodine 400 mcg /day 1 drop applied to skin
EGCG 50%polyphenols green tea extract Jarrow
allicin max 180 mg allicin 1x2x/day
DHEA trace once a day just started last week titrating up
milk thistle 2000 mg 3 days a week

serrapeptase 120,000 SPU
nattokinase 146 mg/day

betaine HCL 650 mg + pepsin 165 mg (1x 2x/day)
digestive enzymes Source Naturals with each meal
Bifidobacterium infantis (align)
Biokult probiotic

I try to walk during the day, 20 to 30 minutes on flat ground, it improves mood but makes sleep worse.

Bedtime around 9:30 pm
Mg taurate 250 mg
herbal tea of linden flower, hops, lemon balm

-First of all : A lot of your initial symptoms (OI, Thyroid, Low Temp) match exactly mine

You take so many supplements, i believe that you should start with the basics :

-Have you checked your Homocysteine levels? How about 25 (OH) D?
-Liver Function Test? Possible NAFLD?
-Despite the fact that you do not have any ER Stress SNPs you could try TUDCA + Selenium + Taurine and see whether you feel better.

Start with the basics : You should find out which Methylation supplements you should take ( you can ask a person who knows more than what i know regarding Methylation). I see that you have many SNPs for GCH1 so Tetrahydrobiopterin is not metabolized

Abstain from any P450 inhibitors (list on my first thread) and Phenylalanine sources.


That should get you started

 

[girlinthesnow]

Interesting you should mention 25 (OH), mine is high 59.7 pg/L
1,25 Di-OH is even more out of range at 112 pg/L (normal range 20.0-75.0) but I did stupidly supplement this on the advice of a nutritionist.

SlgA is low 392 (range 510-2040)

Perforin mRNA 1010 (normal range 250-750)

Prostaglandin E2 23.82 (normal range 0.1-2.81)

VEGF 1.54 (normal range 0.37-1.49)

IL 1beta serum 22 (normal range 0-3)

IL-6 serum 34 (normal range 0-5)

IL-8 serum 4498 (normal range 0-15)

MCP1 798 (normal range 0-165)

MIP-1beta serum 198 (normal range 0-155)

D-Lact 2.68 (range 0-1.66)
L-Lact 2.58 ( range 0.59 - 1.97)

The last liver tests I had done, between courses of pulsed abx, were in range but nevertheless NAFLD wouldn't surprise me.

btw just had another look at your first post and noticed that you didn't tolerate oats. Strangely, nor do I, I am not allergic nor do I have a food intolerance to them but they put me in a stupefied state. Maybe a clue?

I'll try your suggestions and report back

Thanks again for your help!

 

[girlinthesnow]

Further to the above I've just had another trawl through my 23andme results (run through Sterling Hill's app) and found these mutations:

PAH A33429G rs10860936 (Risk C) C;C
PAH A75311G rs1722387 (Risk T) T;T
PAH C35625G rs1522305 (Risk G) G;G
PAH C5594T rs1522296 (Risk A) A;A
PAH G1155C rs772897 (Risk G) G;G
PAH 17864C rs1522307 (Risk G) G;G
PAH 31338A rs11111419 (Risk T) T;T

So it looks like a low protein diet is probably a good idea. Anyone know how an adult could get tested for PKU in the UK?

 

[mariovitali]

Further to the above I've just had another trawl through my 23andme results (run through Sterling Hill's app) and found these mutations:

PAH A33429G rs10860936 (Risk C) C;C
PAH A75311G rs1722387 (Risk T) T;T
PAH C35625G rs1522305 (Risk G) G;G
PAH C5594T rs1522296 (Risk A) A;A
PAH G1155C rs772897 (Risk G) G;G
PAH 17864C rs1522307 (Risk G) G;G
PAH 31338A rs11111419 (Risk T) T;T

So it looks like a low protein diet is probably a good idea. Anyone know how an adult could get tested for PKU in the UK?

Hmmm...yes definitely you should get tested. I believe that you somehow managed so far because you do not have any mutations in ER Stress response. If you would had these mutations too, -in theory- you would be in *serious* trouble.


Also you have some (+/-) SNPs on GCH1 so IMO you should :

-Avoid Phenylalanine sources (Chewing Gum with Aspartame, Aspartame, Egg Whites, Animal Protein)
-Supplement with C
-Supplement with high dose Metafolin


I think you are getting very close to finding what is going on


FWIW, here is mine :


rs10860936 (Risk C) T;T
rs1722387 (Risk C) C;C
rs1522305 (Risk C) G;G
rs1522296 (Risk A) G;G
rs772897 (Risk C) C;C
rs1522307 (Risk G) A;A
rs11111419 (Risk T) A;A


Where did you find risk alleles for PAH? From the app?

 

[girlinthesnow]

Yes, it does feel like an answer, perhaps even 'the' answer. Info was on Sterling Hill's report from her site mthfrsupport.com

 

[Violeta]

From the title of this paper, would it imply that taurine depletion is good or bad? I am thinking it means taurine depletion is not good.

Do you find it interesting that there are amino acids that actually are good for protein folding?

http://www.ncbi.nlm.nih.gov/pubmed/25833528

That was a silly question, I couldn't remember if grp78 was good or bad.

And another good reason to supplement taurine.

http://www.ncbi.nlm.nih.gov/pubmed/11535574

One more just so I have them in one place for rereading later.
http://www.ncbi.nlm.nih.gov/pubmed/9268304

 

[Violeta]

@mariovitali Do you know if sun and heat affect protein folding? I see Vitamin D is supposed to be helpful, but sun and heat seem to have a very definite deleterious effect on me. TIA

 

[Violeta]

I was wondering how high up the problem went. I wonder if it's difficult to clean out the the ER of the hippocampus.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655482/

http://www.jci.org/articles/view/12978

I imagine viruses could cause ER stress in the hippocampus.

http://cshperspectives.cshlp.org/content/5/1/a013250.full

 

[mariovitali]

@mariovitali Do you know if sun and heat affect protein folding? I see Vitamin D is supposed to be helpful, but sun and heat seem to have a very definite deleterious effect on me. TIA

D3 may help some people but it is a double sword for some other people because it helps calcium metabolism. Increased Calcium in a cell that has impaired Calcium homeostasis is not a good idea. (See also the first post on this thread regarding D3)

BTW, have you started the regimen?

 

[JPV]

@mariovitali, would you mind running the term "Microglia" through your software and see if there are any interesting connections to the supplements that you're recommending?

 

[mariovitali]

@mariovitali, would you mind running the term "Microglia" through your software and see if there are any interesting connections to the supplements that you're recommending?

At the moment of writing "microglia" has 20751 entries at PubMed. These were downloaded to my disk (as microglia.csv) and matched against all other topics of Interest. Here are the results :

I will have to make a full run which takes 3 hours to complete.

FYI Benfotiamine comes up to some other type of analysis i am running. It could be either really good or really bad to us ( i believe the second).

microglia.csv : 62.96 %
inducible_nos.csv : 3.95 %
excitotoxicity.csv : 2.49 %
amyloid.csv : 2.13 %
mcp-1.csv : 1.72 %
inflammatory_response.csv : 1.57 %
cerebrovascular_amyloidosis.csv : 1.28 %
peroxynitrite.csv : 1.16 %
ngf.csv : 0.99 %
il_10.csv : 0.81 %
neuronal_nos.csv : 0.73 %
ros.csv : 0.70 %
advanced_glycation_end.csv : 0.66 %
neurite_outgrowth.csv : 0.63 %
benfotiamine.csv : 0.60 %
perk.csv : 0.59 %
tau.csv : 0.57 %
misfolded_proteins.csv : 0.55 %
oxidative_stress_markers.csv : 0.47 %
dopamine_levels.csv : 0.43 %
immune_response.csv : 0.43 %
curcumin.csv : 0.42 %
nadph_human.csv : 0.41 %
allopregnanolone.csv : 0.40 %
glutamate.csv : 0.38 %
resveratrol.csv : 0.36 %
oxidative_stress_protection.csv : 0.34 %
gaba_human.csv : 0.34 %
amyloidosis.csv : 0.33 %
trpv.csv : 0.33 %
gtp_cyclohydrolase.csv : 0.33 %
dopamine.csv : 0.32 %
mitochondrial_dysfunction.csv : 0.31 %
caspase_human.csv : 0.29 %
anhedonia.csv : 0.29 %
er_stress.csv : 0.28 %
gpr78.csv : 0.27 %
l_tryptophan.csv : 0.25 %
pqq.csv : 0.25 %
l-arginine.csv : 0.22 %
hsp70.csv : 0.22 %
tetrahydrobiopterin.csv : 0.22 %
pgc1.csv : 0.21 %
calcium_homeostasis.csv : 0.21 %
xbp1.csv : 0.21 %
rxr.csv : 0.21 %
butyrate.csv : 0.21 %
monosodium_glutamate.csv : 0.21 %
o-glcnac.csv : 0.20 %
vcam-1.csv : 0.20 %
lipoic_acid.csv : 0.19 %
sirt1.csv : 0.19 %
tudca.csv : 0.19 %
glycoproteins.csv : 0.18 %
omega3.csv : 0.17 %
hpa_axis.csv : 0.17 %
endothelial_nos.csv : 0.17 %
histone_deacetylase.csv : 0.17 %
protease_inhibitor.csv : 0.16 %
autism.csv : 0.16 %
heat_shock_protein.csv : 0.16 %
acetylcholine.csv : 0.14 %
limbic_system.csv : 0.14 %
rar.csv : 0.13 %
glycosylation.csv : 0.12 %
glycerylphosphorylcholine.csv : 0.12 %
chaperones.csv : 0.12 %
serotonin_levels.csv : 0.12 %
osmolytes.csv : 0.11 %
ginkgo.csv : 0.11 %
hmgcoa.csv : 0.11 %
cfs.csv : 0.11 %
coenzymeq10.csv : 0.11 %
n-acetylglucosamine.csv : 0.10 %
tocotrienol.csv : 0.10 %
pbmc.csv : 0.10 %
udpglcnac.csv : 0.10 %
pregnenolone.csv : 0.09 %
l-dopa.csv : 0.09 %
p450scc.csv : 0.09 %
choline_deficiency.csv : 0.08 %
adrenal_insufficiency.csv : 0.08 %
pxr.csv : 0.08 %
dysautonomia.csv : 0.07 %
hydroxysteroid_dehydrogenase.csv : 0.07 %
steroidogenesis_human.csv : 0.07 %
dht.csv : 0.07 %
star.csv : 0.06 %
l_tyrosine.csv : 0.06 %
nlinkedglycosylation.csv : 0.06 %
phospholipid_human.csv : 0.06 %
acetyl-coa.csv : 0.05 %
mast_cell_activation.csv : 0.05 %
nadh_human.csv : 0.05 %
5alphareductase.csv : 0.05 %
social_anxiety.csv : 0.04 %
taurine.csv : 0.04 %
triiodothyronine_levels.csv : 0.04 %
upr.csv : 0.04 %
finasteride.csv : 0.04 %
vitamin_d3.csv : 0.03 %
udpgluc.csv : 0.03 %
iron_deficiency.csv : 0.03 %
phenylketonuria.csv : 0.03 %
accutane.csv : 0.03 %
p5p.csv : 0.03 %
selenium_deficiency.csv : 0.03 %
phosphatidylcholine.csv : 0.03 %
testosterone_production.csv : 0.02 %
systemic_amyloidosis.csv : 0.02 %
insomnia.csv : 0.02 %
selenium.csv : 0.02 %
zinc_supplementation.csv : 0.02 %
vitamin_b6.csv : 0.02 %
p450oxidoreductase.csv : 0.02 %
ebv.csv : 0.02 %
urea_cycle.csv : 0.02 %
mastocytosis.csv : 0.02 %
cyp3a4.csv : 0.02 %
cortisol_levels.csv : 0.01 %
steatohepatitis.csv : 0.01 %
crohns_disease.csv : 0.01 %
cimetidine.csv : 0.01 %
l_carnitine.csv : 0.01 %
hepatotoxicity.csv : 0.01 %
cholestasis.csv : 0.01 %
sshl.csv : 0.01 %
human_semen.csv : 0.01 %
insulin_resistance.csv : 0.00 %
adhd.csv : 0.00 %
uric_acid.csv : 0.00 %
ckd.csv : 0.00 %
dolichol.csv : 0.00 %
5-htp.csv : 0.00 %
adrenal_hyperplasia.csv : 0.00 %
scfa.csv : 0.00 %
vitamin_k2.csv : 0.00 %
5mthf.csv : 0.00 %
panic_disorder.csv : 0.00 %
dpagt1.csv : 0.00 %
irritable_bowel.csv : 0.00 %
ire1.csv : 0.00 %
nafld.csv : 0.00 %
3betahsd.csv : 0.00 %
orthostatic_intolerance.csv : 0.00 %
probiotics.csv : 0.00 %
oxalates.csv : 0.00 %
microbiome_humans.csv : 0.00 %
hexosamine.csv : 0.00 %
gluten.csv : 0.00 %
human_proteinuria.csv : 0.00 %
cyp2d6.csv : 0.00 %
fmo3.csv : 0.00 %
sinusitis.csv : 0.00 %
cyp1a2.csv : 0.00 %
beta-alanine.csv : 0.00 %
freet3.csv : 0.00 %
car.csv : 0.00 %
mucuna.csv : 0.00 %
mthfr.csv : 0.00 %
creatine_supplementation.csv : 0.00 %
cyp1b1.csv : 0.00 %
dihydroprogesterone.csv : 0.00 %
cyp2e1.csv : 0.00 %
cyp1a1.csv : 0.00 %
hsc.csv : 0.00 %
floaters.csv : 0.00 %
asymmetric_dimethylarginine.csv : 0.00 %
rituximab.csv : 0.00 %
d-limonene.csv : 0.00 %
resistant_starch.csv : 0.00 %
tinnitus.csv : 0.00 %
hgh.csv : 0.00 %
tmao.csv : 0.00 %
atrial_fibrillation.csv : 0.00 %
magnesium_deficiency.csv : 0.00 %
subclinicalhypo.csv : 0.00 %
srd5a3.csv : 0.00 %

 

[mariovitali]

@JPV, @Violeta , @girlinthesnow

This a csv where i see how many matchings happen : It will probably take time to explain (which i will do in the near future) have a look at the output on the screenshot i attached.

col2,col4,col6,col8,col10,col12 have the number of matchings that have been performed by the software for varying levels of generality (col2=high generality matchings, col12=low generality matchings, col5-col10 varying levels of generality in between)

Notice how from high generality matchings we move from Choline deficiency, er stress, neuronal nos and excitotoxicity to very specific matchings that contain mostly CYPs but also non-alcoholic liver disease.

I can then ask to see the matchings per topic on the top -say- 15 positions (notice that bold letters are for the top 15 matchings - number of matchings is the last number on each line) :

53 er_stress.csv 4
66 gpr78.csv 4
176 xbp1.csv 4
86 ire1.csv 3
120 oxidative_stress_protection.csv 3
139 resveratrol.csv 3
166 tudca.csv 3
38 cyp1a2.csv 2
41 cyp2e1.csv 2
54 excitotoxicity.csv 2
67 gtp_cyclohydrolase.csv 2
82 inducible_nos.csv 2
111 neuronal_nos.csv 2
119 oxidative_stress_markers.csv 2
122 p450scc.csv 2
127 peroxynitrite.csv 2
136 pxr.csv 2
141 ros.csv 2
146 serotonin_levels.csv 2
148 sirt1.csv 2
150 srd5a3.csv 2
160 tetrahydrobiopterin.csv 2
169 upr.csv 2


We can also see the top topics for the top 35 places

file y
66 gpr78.csv 5
86 ire1.csv 5
166 tudca.csv 5
176 xbp1.csv 5
52 endothelial_nos.csv 4
53 er_stress.csv 4
67 gtp_cyclohydrolase.csv 4
82 inducible_nos.csv 4
126 perk.csv 4
127 peroxynitrite.csv 4
141 ros.csv 4
160 tetrahydrobiopterin.csv 4
169 upr.csv 4
23 car.csv 3
38 cyp1a2.csv 3
41 cyp2e1.csv 3
42 cyp3a4.csv 3
54 excitotoxicity.csv 3
92 l-arginine.csv 3
102 mitochondrial_dysfunction.csv 3
108 nadph_human.csv 3
111 neuronal_nos.csv 3
119 oxidative_stress_markers.csv 3
120 oxidative_stress_protection.csv 3
121 p450oxidoreductase.csv 3
122 p450scc.csv 3
128 pgc1.csv 3
136 pxr.csv 3
139 resveratrol.csv 3
148 sirt1.csv 3
150 srd5a3.csv 3
152 star.csv 3
16 asymmetric_dimethylarginine.csv 2
19 benfotiamine.csv 2
22 calcium_homeostasis.csv 2
24 caspase_human.csv 2
29 choline_deficiency.csv 2
36 curcumin.csv 2
37 cyp1a1.csv 2
39 cyp1b1.csv 2
40 cyp2d6.csv 2
47 dopamine_levels.csv 2
49 dpagt1.csv 2
79 hydroxysteroid_dehydrogenase.csv 2
95 lipoic_acid.csv 2
105 mucuna.csv 2
109 nafld.csv 2
125 pbmc.csv 2
146 serotonin_levels.csv 2
154 steroidogenesis_human.csv 2
158 taurine.csv 2


If one would ask me what this all means is that it is very probable that all problems originate from the liver.

 

[JPV]

@mariovitali

Thanks for taking to time to run this analysis. Overactive Microglia is discussed a lot by many top ME/CFS researchers and is a topic that comes up frequently on the forum.

It seems that several of the supplements that you recommend, for ER Stress, also have a positive effect on Microglia. It makes me wonder if maybe Microglia is the main issue that your protocol is addressing.

 

[Violeta]

D3 may help some people but it is a double sword for some other people because it helps calcium metabolism. Increased Calcium in a cell that has impaired Calcium homeostasis is not a good idea. (See also the first post on this thread regarding D3)

BTW, have you started the regimen?

Yes, I did see that about calcium regulation with respect to D3, maybe that's the link to sun and heat.

I had been doing several of the things from the protocol, such as choline, K2 for calcium, Taurox 6B for homeopathic taurine and beta alanine + calcarea cabonica for calcium regulation, working on methylation from the standpoint of correcting B2 deficiency, added taurine after reading the first post of the thread, already avoid most of the things that you recommend avoiding. This week I have been changing diet, removing fish and using low purine protein, eggs and cheese.

Thanks for all your help.

 

[Gondwanaland]

If one would ask me what this all means is that it is very probable that all problems originate from the liver.

Mario, I think that an "injured" microbiome (by meds, pesticides and inumerous poisons) plays a large role here as well, since the gut bacteria supply the liver with the needed enzymes and vitamins.

 

[Violeta]

I have been reading the thread about aluminum and wondered if it was relative to endoplasmic reticulum stress and found this.

http://www.ncbi.nlm.nih.gov/pubmed/8151628

 

[Violeta]

Finasterde contains "aluminum lake", which is a food coloring. Since it sure looks like aluminum, I looked it up and sources say it doesn't contain aluminum, it contains "aluminum oxide". What do you think?

Doesn't look good.
PROSCAR (finasteride) tablets for oral administration are film-coated tablets that contain 5 mg of finasteride and the following inactive ingredients: hydrous lactose, microcrystalline cellulose, pregelatinized starch, sodium starch glycolate, hydroxypropyl cellulose LF, hydroxypropyl methylcellulose, titanium dioxide, magnesium stearate, talc, docusate sodium, FD&C Blue 2 aluminum lake and yellow iron oxide.

And here..
http://www.naturalnews.com/034813_childrens_medicines_aluminum_pills.html