He seems to believe that the viral RNA is attaching itself to the DNA in the cell. He doesn't go into much detail about this but mentions that it's a phenomenon that he was aware of.Did Dr Chia say why human DNA interferes with the PCR testing of enterovirus? I know nothing about the technicalities of PCR testing, but I am wondering if this interference is a common occurrence, or whether is it something specific to enteroviruses, or perhaps specific to intracellular infections (enterovirus of course can create long-term intracellular infections).
I don't believe this was determined. The samples were ground and kept in a medium. The samples were taken from the pontomedullary junction, medial temporal lobe, lateral frontal cortex, occipital lobe, cerebellum, and midbrain. I had a bit of trouble following this part of the talk, but it appears that they incubated these tissue samples with some of his serum and then did a western blot that showed reactivity in all six samples. For some reason, after applying DNAse, the RT-PCR only came back positive for enterovirus in the pontomedullary junction and frontal cortex.Did Dr Chia say which cell types of the brain tested positive for enterovirus, or wasn't this determined? In one of the two previous ME/CFS brain autopsies (the Richardson autopsy), it was found that the glial cells of the brain, plus the fibroblast cells around small vessels of the brain, stained positive for enteroviral VPI protein.