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What autoantibodies and ANAs tests should I do?

ChookityPop

Senior Member
Messages
583
I want to do all the relevant/potential relevant tests I can do.

I have POTS and have trouble with blood pooling. I have also had two anaphylactic shocks and have a lot of allergies, so I think I have MCAS. I eat basically carnivore and I think that have eliminated potential anaphylactic episodes.. Currently trying mestinon. I want to try IVIG and potentially rituximab.
 

Pyrrhus

Senior Member
Messages
4,172
Location
U.S., Earth
I have POTS and have trouble with blood pooling.

There are tests you can do to determine if you have auto-antibodies such as anti-adrenergic antibodies. If you do have them, though, there may not be much that you can do about it except IVIG.

I have also had two anaphylactic shocks and have a lot of allergies, so I think I have MCAS.

Anaphylactic episodes definitely signal problems with mast cells, but I don't know much about testing or treatment...
 

Learner1

Senior Member
Messages
6,305
Location
Pacific Northwest
Attached is a list of tests that patients on this forum have had done.

IVIG can be used to help with autoimmunity. Aside from that you can take drugs and/or supplements to help manage symptoms. And, Rituximab can be used to teach the body to stop making autoimmune antibodies that are causing problems.
 

Attachments

  • Antibody tests for ME_CFS patients.pdf
    69.5 KB · Views: 99

ChookityPop

Senior Member
Messages
583
Attached is a list of tests that patients on this forum have had done.

IVIG can be used to help with autoimmunity. Aside from that you can take drugs and/or supplements to help manage symptoms. And, Rituximab can be used to teach the body to stop making autoimmune antibodies that are causing problems.

Awesome, thanks! Is it possible to take antibodies for autoimmunity through my dr or is it something an immunological dr does?
 

Cipher

Administrator
Messages
838
Where are the instructions for this, please?

On their website there are two separate links to PDFs with instructions. One is called "labservice" and the other is called "sample preparation and logistics". The PDF-links are right under the list of the auto-antibody they analyze.
 

sometexan84

Senior Member
Messages
1,229
For the Celltrend CFS diagnostics, I had my specimen collection done w/out a doctor at Any Lab Test Now, and they sent it to Celltrend. I just had to tell ALTN in advance what I was wanting to do, and I had to print off the lab service and sample prep instructions from the Celltrend pg, and bring it in to ALTN.

I'll let you know if I receive abnormal results for:
  • Beta-1 adrenergic receptor auto-antibodies
  • Beta-2 adrenergic receptor auto-antibodies
  • Muscarinic cholinergic (M3) receptor auto-antibodies
  • Muscarinic cholinergic (M4) receptor auto-antibodies
 

xebex

Senior Member
Messages
840
I’m thinking of getting antibody test too but what is the end purpose of it? Is simply just to prove your condition or is it for access to specific medications that would target the specific issue?
 

sometexan84

Senior Member
Messages
1,229
I’m thinking of getting antibody test too but what is the end purpose of it? Is simply just to prove your condition or is it for access to specific medications that would target the specific issue?
I am doing it for further diagnosis and treatment. I'm looking for conditions I can treat. I'm looking for autoantibodies I can treat (e.g. Selenium to remove TPO antibodies). And I am looking for further insight into my overall condition.

For instance, I just found out I have "High" Phosphatidylserine antibodies. Now I need to go test for lupus anticoagulant and Beta-2 glycoprotein 1 antibodies to see if I have Antiphospholipid Syndrome, which is talked about here “Sticky Blood” – Antiphospholipid Syndrome, POTS, Chronic Fatigue Syndrome and Fibromyalgia – The Dysautonomia Conference #4

And since Parvovirus B19 causes Phosphatidylserine antibodies....

Similarities of specificity and cofactor dependence in serum antiphospholipid antibodies from patients with human parvovirus B19 infection and from those with systemic lupus erythematosus
Antiphospholipid antibodies in pediatric and adult patients with rheumatic disease are associated with parvovirus B19 infection

... I now need to check for other autoantibodies produced by B19 like...

Autoantibodies produced by B19

1606180752080-png.40560



1606180771099-png.40561



1606180786474-png.40562



B19 IgG in serum cross-reacting to human autoantigens

1606181400222-png.40563


If I test positive for something from CellTrends, then I guess I'll need to find out what causes beta-2 adrenergic receptor auto-antibodies, which I do not currently know.

Though, I did find an article showing how a co-infection of Coxsackie and Streptococcus can cause beta-1 (not beta-2) adrenergic receptor auto-antibodies.

BTW - I have a IgG titer to Parvovirus B19 that suggest past infection, not current active infection. But any autoantibodies created by B19 could still exist in the body. And apparently the actual B19 IgG antibodies in the body can cross react to human auto-antigens. ALSO, I'm not yet convinced that IgG and IgM tests confirm whether or not there is an active B19 infection.
 
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Learner1

Senior Member
Messages
6,305
Location
Pacific Northwest
If I test positive for something from CellTrends, then I guess I'll need to find out what causes beta-2 adrenergic receptor auto-antibodies, which I do not currently know.
Epstein-Barr and other herpes family viruses can, but many different infections can triggered many types of antibodies. Most are B cell antibodies.

Hashimotos autoimmune thyroid disease is different, it's T cell driven, not B cell driven.

https://www.nature.com/articles/nm0...ng insidious, progressive thyroid destruction.

I’m thinking of getting antibody test too but what is the end purpose of it? Is simply just to prove your condition or is it for access to specific medications that would target the specific issue?
For most autoimmune diseases, treatment is either to help symptoms. Treatment would depend on the antibodies and autoimmune problem found.

Or to suppress the immune system from reacting, which can gave negative consequences, like cancer and inability to fight infections.

Or a treatment like Rituximab can be used to wipe out B cell production for awhile, to try to teach the immune system not to make the bad antibodies. This is not specific to the type of antibodies and has been used with success in several types of autoummunity. There are other similar drugs that attack different subsets of B cells. Rituximab just does CD20 cells.

Another strategy for wiping out B cells is plasmapharesis, a mechanical process for vacuuming B cells out of the blood, which works until the body makes more B cells, so it has to be repeated with some frequency.
 

sometexan84

Senior Member
Messages
1,229
Epstein-Barr and other herpes family viruses can
I have actually Googled this specifically and found nothing that says EBV can produce (or lead to the creation of) ß2 adrenergic receptor antibodies. If you have proof of this, I'd like to add it to my notes.
1606358182640.png
 
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xebex

Senior Member
Messages
840
Is it to do with the theory that’s EBV plays a part in POTs and POTS is showing issues with these receptors too?
 

Learner1

Senior Member
Messages
6,305
Location
Pacific Northwest
I have actually Googled this specifically and found nothing that says EBV can produce (or lead to the creation of) ß2 adrenergic receptor antibodies. If you have proof of this, I'd like to add it to my notes.
1606358182640-png.40584
My doctor, who is a top ME/CFS specialist, suggested this, and I found it to be true by reading the science. See the attached and this:

https://www.ihsymposium.com/session/herpes-virus-and-autoimmunity/
Is it to do with the theory that’s EBV plays a part in POTs and POTS is showing issues with these receptors too?
Yes. See the attached.
 

Attachments

  • EBV and autoimmune disease.pdf
    625.8 KB · Views: 22
  • 1-s2.0-S1198743X16304293-main.pdf
    842.9 KB · Views: 16
  • autoimmune basis of POTS - adrenergic antibodies.pdf
    1.1 MB · Views: 17
  • Adrenergic antibodies POTS.pdf
    323.2 KB · Views: 17
  • adrenergic muscarinic antibodies in ME CFS.pdf
    1.9 MB · Views: 16
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sometexan84

Senior Member
Messages
1,229
Is there something online that shows the direct association between ebv and beta 2 receptor?
 
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Learner1

Senior Member
Messages
6,305
Location
Pacific Northwest
  1. EBV (and other herpes viruses) trigger autoimmunity
  2. The autoimmunity they trigger includes POTS causing antibodies
  3. The POTS causing antibodies create POTS
I learned all this by reading the papers after my doctor suggested this and tested me for the POTS causing antibodies.