Unfolded Protein Response and A Possible Treatment for CFS

[Violeta]

In the B2 I love you thread I saw a hint that B2, Riboflavin, has something to do with endoplasmic reticulum, so I thought I would look it up and found this.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201499/

Riboflavin Deficiency Impairs Oxidative Folding and Secretion of Apolipoprotein B-100 in HepG2 Cells, Triggering Stress Response Systems

 

[Violeta]

Another oh, gee, I wonder if...
raised liver enzymes are a sign of endoplasmic reticulum stress.

"Their serum levels reflect the amount of hepatocellular injury and death on a day-by-day basis. Aminotransferases (and predominantly AST) are not only found in hepatocytes but also in other tissues (heart and skeletal muscles, kidney, brain, pancreas, lung, and red blood cells). The liver contains 400 U ALT/g protein (mainly cytoplasmic) and 500 U AST/g protein (> 80 3 % contained in mitochondria and endoplasmic reticulum). Damage to one gram of liver tissue (or the membranes of 171 million hepatocytes) results in a significant increase in the serum ALT activity (3, 4). AST responds in the same fashion, especially following liver cell necrosis and destruction of mitochondria and endoplasmic reticulum.

 

[mariovitali]

@Violeta


Note : hsc.csv = hepatic stellate cell

Alanine aminotransferase : ALT

*********Topic : alanine aminotransferase ***************
nafld.csv : 8.41 %
hepatotoxicity.csv : 8.37 %
steatohepatitis.csv : 2.98 %
hsc.csv : 2.94 %
cyp2e1.csv : 2.51 %
choline_deficiency.csv : 1.94 %
p5p.csv : 1.48 %
hepatocytes.csv : 1.42 %
hmgb1.csv : 1.25 %
oxidative_stress_markers.csv : 1.11 %
cholestasis.csv : 1.11 %
inducible_nos.csv : 0.88 %
insulin_resistance.csv : 0.76 %
tudca.csv : 0.76 %
uric_acid.csv : 0.75 %
creatine_supplementation.csv : 0.69 %
vitamin_b6.csv : 0.64 %
oxidative_stress_protection.csv : 0.64 %
mucuna.csv : 0.55 %
p450oxidoreductase.csv : 0.49 %
zinc_supplementation.csv : 0.49 %
il_10.csv : 0.46 %
urea_cycle.csv : 0.45 %
udpgluc.csv : 0.41 %
fmo3.csv : 0.40 %
peroxynitrite.csv : 0.40 %
curcumin.csv : 0.37 %
cyp1a2.csv : 0.36 %
gpr78.csv : 0.35 %
inflammatory_response.csv : 0.35 %
ginkgo.csv : 0.34 %
mcp-1.csv : 0.32 %
cox-2.csv : 0.31 %
l_carnitine.csv : 0.31 %
hsp70.csv : 0.29 %
acetyl-coa.csv : 0.28 %
cyp1a1.csv : 0.28 %
ros.csv : 0.27 %
taurine.csv : 0.27 %
monosodium_glutamate.csv : 0.26 %
hmgcoa.csv : 0.26 %
selenium.csv : 0.26 %
selenium_deficiency.csv : 0.26 %
mitochondrial_dysfunction.csv : 0.25 %
protease_inhibitor.csv : 0.25 %
triiodothyronine_levels.csv : 0.24 %
glutamate.csv : 0.24 %
resveratrol.csv : 0.23 %
probiotics.csv : 0.21 %
er_stress.csv : 0.21 %
xbp1.csv : 0.21 %
d-limonene.csv : 0.21 %
ckd.csv : 0.20 %
car.csv : 0.19 %
sirt1.csv : 0.19 %
endothelial_nos.csv : 0.19 %
caloric_restriction.csv : 0.18 %
l-arginine.csv : 0.18 %
coenzymeq10.csv : 0.18 %
vcam-1.csv : 0.17 %
omega3.csv : 0.17 %
heat_shock_protein.csv : 0.16 %
cortisol_levels.csv : 0.16 %
cyp3a4.csv : 0.15 %
immune_response.csv : 0.15 %
pxr.csv : 0.15 %
scfa.csv : 0.14 %
cyp2d6.csv : 0.14 %
perk.csv : 0.14 %
l_tryptophan.csv : 0.14 %
ebv.csv : 0.13 %
human_proteinuria.csv : 0.13 %
advanced_glycation_end.csv : 0.13 %
star.csv : 0.12 %
subclinicalhypo.csv : 0.12 %
glycerylphosphorylcholine.csv : 0.12 %
caspase_human.csv : 0.11 %
beta-alanine.csv : 0.11 %
resistant_starch.csv : 0.11 %
ire1.csv : 0.11 %
isotretinoin.csv : 0.11 %
pbmc.csv : 0.10 %
tocotrienol.csv : 0.10 %
lipoic_acid.csv : 0.10 %
stat1.csv : 0.09 %
nadh_human.csv : 0.09 %
l_tyrosine.csv : 0.09 %
rituximab.csv : 0.09 %
cfs.csv : 0.09 %
pregnenolone.csv : 0.09 %
p450scc.csv : 0.09 %
hydroxysteroid_dehydrogenase.csv : 0.07 %
insomnia.csv : 0.07 %
iron_deficiency.csv : 0.07 %
gluten.csv : 0.07 %
cimetidine.csv : 0.07 %
serotonin_levels.csv : 0.07 %
tetrahydrobiopterin.csv : 0.06 %
oxalates.csv : 0.06 %
rxr.csv : 0.06 %
adrenal_hyperplasia.csv : 0.06 %
upr.csv : 0.06 %
dolichol.csv : 0.06 %
mthfr.csv : 0.06 %
phosphatidylcholine.csv : 0.05 %
cyp1b1.csv : 0.05 %
calcium_homeostasis.csv : 0.05 %
neuronal_nos.csv : 0.05 %
hgh.csv : 0.05 %
chaperones.csv : 0.05 %
3betahsd.csv : 0.05 %
vitamin_k2.csv : 0.05 %
phospholipid_human.csv : 0.04 %
asymmetric_dimethylarginine.csv : 0.04 %
atrial_fibrillation.csv : 0.04 %
anhedonia.csv : 0.04 %
p53.csv : 0.04 %
nadph_human.csv : 0.04 %
irritable_bowel.csv : 0.04 %
butyrate.csv : 0.04 %
vitamin_d3.csv : 0.04 %
crohns_disease.csv : 0.04 %
dht.csv : 0.03 %
n-acetylglucosamine.csv : 0.03 %
amyloidosis.csv : 0.03 %
gaba_human.csv : 0.03 %
freet3.csv : 0.03 %
dopamine_levels.csv : 0.03 %
amyloid.csv : 0.03 %
human_semen.csv : 0.03 %
rar.csv : 0.03 %
testosterone_production.csv : 0.02 %
microbiome_humans.csv : 0.02 %
steroidogenesis_human.csv : 0.02 %
5alphareductase.csv : 0.02 %
trpv.csv : 0.02 %
glycosylation.csv : 0.02 %
tau.csv : 0.02 %
adrenal_insufficiency.csv : 0.02 %
dysautonomia.csv : 0.02 %
histone_deacetylase.csv : 0.02 %
ngf.csv : 0.02 %
adrenergic_receptor.csv : 0.02 %
inositol.csv : 0.02 %
glycoproteins.csv : 0.01 %
phenylketonuria.csv : 0.01 %
acetylcholine.csv : 0.01 %
dopamine.csv : 0.01 %
tinnitus.csv : 0.01 %
microglia.csv : 0.01 %
l-dopa.csv : 0.01 %
social_anxiety.csv : 0.01 %
sshl.csv : 0.01 %
sinusitis.csv : 0.00 %
5-htp.csv : 0.00 %
mastocytosis.csv : 0.00 %
limbic_system.csv : 0.00 %
5mthf.csv : 0.00 %
panic_disorder.csv : 0.00 %
adhd.csv : 0.00 %
dpagt1.csv : 0.00 %
pgc1.csv : 0.00 %
gtp_cyclohydrolase.csv : 0.00 %
misfolded_proteins.csv : 0.00 %
nlinkedglycosylation.csv : 0.00 %
mast_cell_activation.csv : 0.00 %
insp3.csv : 0.00 %
autism.csv : 0.00 %
orthostatic_intolerance.csv : 0.00 %
cerebrovascular_amyloidosis.csv : 0.00 %
systemic_amyloidosis.csv : 0.00 %
udpglcnac.csv : 0.00 %
finasteride.csv : 0.00 %
hexosamine.csv : 0.00 %
benfotiamine.csv : 0.00 %
baroreceptor.csv : 0.00 %
o-glcnacylation.csv : 0.00 %
neurite_outgrowth.csv : 0.00 %
pqq.csv : 0.00 %
allopregnanolone.csv : 0.00 %
dihydroprogesterone.csv : 0.00 %
osmolytes.csv : 0.00 %
floaters.csv : 0.00 %
excitotoxicity.csv : 0.00 %
hpa_axis.csv : 0.00 %
tmao.csv : 0.00 %
magnesium_deficiency.csv : 0.00 %
o-glcnac.csv : 0.00 %
srd5a3.csv : 0.00 %

then we have aspartate aminotransferase (AST)

*********Topic : aspartate aminotransferase ***************
p5p.csv : 6.28 %
hepatotoxicity.csv : 4.97 %
nafld.csv : 4.02 %
creatine_supplementation.csv : 1.69 %
hsc.csv : 1.64 %
vitamin_b6.csv : 1.60 %
cyp2e1.csv : 1.44 %
steatohepatitis.csv : 1.43 %
uric_acid.csv : 0.84 %
oxidative_stress_markers.csv : 0.79 %
hepatocytes.csv : 0.78 %
cholestasis.csv : 0.75 %
oxidative_stress_protection.csv : 0.61 %
hmgb1.csv : 0.57 %
mucuna.csv : 0.55 %
fmo3.csv : 0.54 %
inducible_nos.csv : 0.52 %
choline_deficiency.csv : 0.43 %
glutamate.csv : 0.43 %
curcumin.csv : 0.42 %
selenium_deficiency.csv : 0.41 %
urea_cycle.csv : 0.40 %
tudca.csv : 0.38 %
ginkgo.csv : 0.37 %
monosodium_glutamate.csv : 0.36 %
oxalates.csv : 0.32 %
acetyl-coa.csv : 0.31 %
zinc_supplementation.csv : 0.30 %
beta-alanine.csv : 0.30 %
tmao.csv : 0.29 %
insulin_resistance.csv : 0.28 %
l_carnitine.csv : 0.27 %
selenium.csv : 0.26 %
p450oxidoreductase.csv : 0.26 %
triiodothyronine_levels.csv : 0.25 %
mitochondrial_dysfunction.csv : 0.25 %
taurine.csv : 0.25 %
cyp1a1.csv : 0.24 %
isotretinoin.csv : 0.24 %
peroxynitrite.csv : 0.23 %
resistant_starch.csv : 0.22 %
gpr78.csv : 0.22 %
l_tyrosine.csv : 0.22 %
resveratrol.csv : 0.22 %
hsp70.csv : 0.22 %
lipoic_acid.csv : 0.21 %
l_tryptophan.csv : 0.21 %
cox-2.csv : 0.20 %
testosterone_production.csv : 0.20 %
ros.csv : 0.19 %
il_10.csv : 0.19 %
inflammatory_response.csv : 0.19 %
udpgluc.csv : 0.18 %
hmgcoa.csv : 0.18 %
perk.csv : 0.17 %
cyp1a2.csv : 0.17 %
caloric_restriction.csv : 0.16 %
sirt1.csv : 0.16 %
nadh_human.csv : 0.16 %
pxr.csv : 0.15 %
cortisol_levels.csv : 0.14 %
xbp1.csv : 0.14 %
mcp-1.csv : 0.14 %
heat_shock_protein.csv : 0.13 %
l-arginine.csv : 0.13 %
star.csv : 0.12 %
cyp3a4.csv : 0.12 %
probiotics.csv : 0.12 %
advanced_glycation_end.csv : 0.11 %
ire1.csv : 0.11 %
coenzymeq10.csv : 0.11 %
protease_inhibitor.csv : 0.10 %
endothelial_nos.csv : 0.10 %
tocotrienol.csv : 0.10 %
car.csv : 0.10 %
ckd.csv : 0.09 %
p450scc.csv : 0.09 %
er_stress.csv : 0.08 %
omega3.csv : 0.08 %
upr.csv : 0.08 %
serotonin_levels.csv : 0.08 %
human_proteinuria.csv : 0.07 %
caspase_human.csv : 0.07 %
nadph_human.csv : 0.07 %
butyrate.csv : 0.07 %
chaperones.csv : 0.07 %
ebv.csv : 0.07 %
calcium_homeostasis.csv : 0.07 %
cfs.csv : 0.06 %
osmolytes.csv : 0.06 %
vcam-1.csv : 0.06 %
dolichol.csv : 0.06 %
mthfr.csv : 0.06 %
5-htp.csv : 0.06 %
gluten.csv : 0.05 %
cyp1b1.csv : 0.05 %
cyp2d6.csv : 0.05 %
dht.csv : 0.05 %
neuronal_nos.csv : 0.05 %
systemic_amyloidosis.csv : 0.05 %
cimetidine.csv : 0.05 %
freet3.csv : 0.05 %
insomnia.csv : 0.05 %
glycosylation.csv : 0.05 %
asymmetric_dimethylarginine.csv : 0.04 %
rituximab.csv : 0.04 %
dopamine_levels.csv : 0.04 %
anhedonia.csv : 0.04 %
subclinicalhypo.csv : 0.04 %
vitamin_d3.csv : 0.04 %
immune_response.csv : 0.04 %
microbiome_humans.csv : 0.04 %
gaba_human.csv : 0.04 %
phosphatidylcholine.csv : 0.03 %
hydroxysteroid_dehydrogenase.csv : 0.03 %
stat1.csv : 0.03 %
hgh.csv : 0.03 %
n-acetylglucosamine.csv : 0.02 %
p53.csv : 0.02 %
amyloidosis.csv : 0.02 %
phospholipid_human.csv : 0.02 %
trpv.csv : 0.02 %
magnesium_deficiency.csv : 0.02 %
atrial_fibrillation.csv : 0.02 %
tau.csv : 0.02 %
crohns_disease.csv : 0.02 %
adrenergic_receptor.csv : 0.02 %
dysautonomia.csv : 0.02 %
iron_deficiency.csv : 0.02 %
social_anxiety.csv : 0.02 %
dopamine.csv : 0.02 %
human_semen.csv : 0.02 %
pregnenolone.csv : 0.01 %
amyloid.csv : 0.01 %
ngf.csv : 0.01 %
neurite_outgrowth.csv : 0.01 %
inositol.csv : 0.01 %
tinnitus.csv : 0.01 %
adrenal_hyperplasia.csv : 0.01 %
l-dopa.csv : 0.01 %
acetylcholine.csv : 0.01 %
adrenal_insufficiency.csv : 0.01 %
histone_deacetylase.csv : 0.01 %
pbmc.csv : 0.01 %
glycoproteins.csv : 0.01 %
sshl.csv : 0.01 %
microglia.csv : 0.00 %
sinusitis.csv : 0.00 %
autism.csv : 0.00 %
mastocytosis.csv : 0.00 %
scfa.csv : 0.00 %
limbic_system.csv : 0.00 %
5mthf.csv : 0.00 %
panic_disorder.csv : 0.00 %
adhd.csv : 0.00 %
dpagt1.csv : 0.00 %
pgc1.csv : 0.00 %
phenylketonuria.csv : 0.00 %
irritable_bowel.csv : 0.00 %
rxr.csv : 0.00 %
5alphareductase.csv : 0.00 %
gtp_cyclohydrolase.csv : 0.00 %
misfolded_proteins.csv : 0.00 %
3betahsd.csv : 0.00 %
nlinkedglycosylation.csv : 0.00 %
mast_cell_activation.csv : 0.00 %
insp3.csv : 0.00 %
orthostatic_intolerance.csv : 0.00 %
cerebrovascular_amyloidosis.csv : 0.00 %
udpglcnac.csv : 0.00 %
finasteride.csv : 0.00 %
hexosamine.csv : 0.00 %
glycerylphosphorylcholine.csv : 0.00 %
benfotiamine.csv : 0.00 %
baroreceptor.csv : 0.00 %
rar.csv : 0.00 %
o-glcnacylation.csv : 0.00 %
pqq.csv : 0.00 %
allopregnanolone.csv : 0.00 %
tetrahydrobiopterin.csv : 0.00 %
steroidogenesis_human.csv : 0.00 %
vitamin_k2.csv : 0.00 %
dihydroprogesterone.csv : 0.00 %
floaters.csv : 0.00 %
d-limonene.csv : 0.00 %
excitotoxicity.csv : 0.00 %
hpa_axis.csv : 0.00 %
o-glcnac.csv : 0.00 %
srd5a3.csv : 0.00 %

finally GGT :

*********Topic : gamma-glutamyl transpeptidase ***************
nafld.csv : 0.67 %
cholestasis.csv : 0.56 %
hexosamine.csv : 0.45 %
tocotrienol.csv : 0.39 %
hepatotoxicity.csv : 0.37 %
steatohepatitis.csv : 0.33 %
hepatocytes.csv : 0.24 %
tudca.csv : 0.19 %
oxidative_stress_markers.csv : 0.19 %
uric_acid.csv : 0.18 %
glutamate.csv : 0.17 %
choline_deficiency.csv : 0.16 %
monosodium_glutamate.csv : 0.15 %
taurine.csv : 0.15 %
oxidative_stress_protection.csv : 0.14 %
cyp2e1.csv : 0.11 %
tmao.csv : 0.10 %
lipoic_acid.csv : 0.10 %
udpgluc.csv : 0.09 %
selenium.csv : 0.09 %
p450scc.csv : 0.09 %
selenium_deficiency.csv : 0.08 %
triiodothyronine_levels.csv : 0.07 %
insulin_resistance.csv : 0.07 %
3betahsd.csv : 0.07 %
butyrate.csv : 0.07 %
cyp1a2.csv : 0.06 %
p450oxidoreductase.csv : 0.06 %
human_semen.csv : 0.06 %
p5p.csv : 0.05 %
cyp1a1.csv : 0.05 %
testosterone_production.csv : 0.05 %
urea_cycle.csv : 0.05 %
human_proteinuria.csv : 0.05 %
ros.csv : 0.05 %
hydroxysteroid_dehydrogenase.csv : 0.05 %
advanced_glycation_end.csv : 0.05 %
5alphareductase.csv : 0.05 %
asymmetric_dimethylarginine.csv : 0.04 %
zinc_supplementation.csv : 0.04 %
glycosylation.csv : 0.04 %
phospholipid_human.csv : 0.04 %
inducible_nos.csv : 0.03 %
dht.csv : 0.03 %
oxalates.csv : 0.03 %
mitochondrial_dysfunction.csv : 0.03 %
cortisol_levels.csv : 0.03 %
omega3.csv : 0.03 %
caloric_restriction.csv : 0.03 %
dopamine_levels.csv : 0.03 %
l-dopa.csv : 0.03 %
l_tyrosine.csv : 0.03 %
cyp3a4.csv : 0.03 %
nadph_human.csv : 0.03 %
pregnenolone.csv : 0.03 %
ckd.csv : 0.03 %
l-arginine.csv : 0.03 %
protease_inhibitor.csv : 0.03 %
mcp-1.csv : 0.02 %
steroidogenesis_human.csv : 0.02 %
l_tryptophan.csv : 0.02 %
hmgcoa.csv : 0.02 %
vitamin_b6.csv : 0.02 %
serotonin_levels.csv : 0.02 %
adrenal_hyperplasia.csv : 0.02 %
vitamin_d3.csv : 0.02 %
inflammatory_response.csv : 0.02 %
phosphatidylcholine.csv : 0.02 %
excitotoxicity.csv : 0.02 %
n-acetylglucosamine.csv : 0.02 %
il_10.csv : 0.02 %
cimetidine.csv : 0.02 %
probiotics.csv : 0.02 %
mastocytosis.csv : 0.02 %
cfs.csv : 0.02 %
caspase_human.csv : 0.01 %
gluten.csv : 0.01 %
vcam-1.csv : 0.01 %
peroxynitrite.csv : 0.01 %
ebv.csv : 0.01 %
resveratrol.csv : 0.01 %
pbmc.csv : 0.01 %
heat_shock_protein.csv : 0.01 %
dopamine.csv : 0.01 %
p53.csv : 0.01 %
endothelial_nos.csv : 0.01 %
nadh_human.csv : 0.01 %
immune_response.csv : 0.01 %
hsp70.csv : 0.01 %
er_stress.csv : 0.01 %
cox-2.csv : 0.01 %
amyloidosis.csv : 0.01 %
gaba_human.csv : 0.01 %
acetyl-coa.csv : 0.01 %
l_carnitine.csv : 0.01 %
glycoproteins.csv : 0.01 %
histone_deacetylase.csv : 0.01 %
social_anxiety.csv : 0.01 %
adrenergic_receptor.csv : 0.01 %
insomnia.csv : 0.01 %
iron_deficiency.csv : 0.01 %
ngf.csv : 0.01 %
inositol.csv : 0.01 %
microglia.csv : 0.00 %
chaperones.csv : 0.00 %
calcium_homeostasis.csv : 0.00 %
amyloid.csv : 0.00 %
tau.csv : 0.00 %
dolichol.csv : 0.00 %
5-htp.csv : 0.00 %
scfa.csv : 0.00 %
isotretinoin.csv : 0.00 %
limbic_system.csv : 0.00 %
coenzymeq10.csv : 0.00 %
sirt1.csv : 0.00 %
5mthf.csv : 0.00 %
panic_disorder.csv : 0.00 %
neuronal_nos.csv : 0.00 %
adhd.csv : 0.00 %
dpagt1.csv : 0.00 %
stat1.csv : 0.00 %
pgc1.csv : 0.00 %
hmgb1.csv : 0.00 %
phenylketonuria.csv : 0.00 %
irritable_bowel.csv : 0.00 %
sshl.csv : 0.00 %
rxr.csv : 0.00 %
ire1.csv : 0.00 %
gtp_cyclohydrolase.csv : 0.00 %
misfolded_proteins.csv : 0.00 %
upr.csv : 0.00 %
nlinkedglycosylation.csv : 0.00 %
mast_cell_activation.csv : 0.00 %
insp3.csv : 0.00 %
gpr78.csv : 0.00 %
xbp1.csv : 0.00 %
autism.csv : 0.00 %
orthostatic_intolerance.csv : 0.00 %
cerebrovascular_amyloidosis.csv : 0.00 %
systemic_amyloidosis.csv : 0.00 %
microbiome_humans.csv : 0.00 %
udpglcnac.csv : 0.00 %
adrenal_insufficiency.csv : 0.00 %
perk.csv : 0.00 %
finasteride.csv : 0.00 %
glycerylphosphorylcholine.csv : 0.00 %
benfotiamine.csv : 0.00 %
anhedonia.csv : 0.00 %
cyp2d6.csv : 0.00 %
fmo3.csv : 0.00 %
baroreceptor.csv : 0.00 %
rar.csv : 0.00 %
o-glcnacylation.csv : 0.00 %
crohns_disease.csv : 0.00 %
neurite_outgrowth.csv : 0.00 %
sinusitis.csv : 0.00 %
pqq.csv : 0.00 %
beta-alanine.csv : 0.00 %
freet3.csv : 0.00 %
car.csv : 0.00 %
mucuna.csv : 0.00 %
dysautonomia.csv : 0.00 %
ginkgo.csv : 0.00 %
allopregnanolone.csv : 0.00 %
tetrahydrobiopterin.csv : 0.00 %
mthfr.csv : 0.00 %
vitamin_k2.csv : 0.00 %
curcumin.csv : 0.00 %
creatine_supplementation.csv : 0.00 %
cyp1b1.csv : 0.00 %
dihydroprogesterone.csv : 0.00 %
hsc.csv : 0.00 %
osmolytes.csv : 0.00 %
floaters.csv : 0.00 %
acetylcholine.csv : 0.00 %
rituximab.csv : 0.00 %
d-limonene.csv : 0.00 %
resistant_starch.csv : 0.00 %
trpv.csv : 0.00 %
tinnitus.csv : 0.00 %
hpa_axis.csv : 0.00 %
hgh.csv : 0.00 %
atrial_fibrillation.csv : 0.00 %
magnesium_deficiency.csv : 0.00 %
subclinicalhypo.csv : 0.00 %
o-glcnac.csv : 0.00 %
star.csv : 0.00 %
srd5a3.csv : 0.00 %
pxr.csv : 0.00 %

 

[Violeta]

Thank you, @mariovitali , for taking the time to do that. So none of them are related to misfolded proteins in literature, but at the same time they are seen with heat shock protein and a few of the other related things such as hmgb1 and perk. Finasteride, which so far I have only placed in one list is 00.00%!


The only enzyme that I have had raised is AST, and only mildly.

Have you had your liver enzymes checked? Anyone?

 

[mariovitali]

Thank you, @mariovitali , for taking the time to do that. So none of them are related to misfolded proteins in literature, but at the same time they are seen with heat shock protein and a few of the other related things such as hmgb1 and perk. Finasteride, which so far I have only placed in one list is 00.00%!


The only enzyme that I have had raised is AST, and only mildly.

Have you had your liver enzymes checked? Anyone?

No i never had them tested, this is on my TODO list. But maybe it's too late now ;-)

Regarding Finasteride this is how it looks like :

*********Topic : finasteride ***************
finasteride.csv : 79.90 %
5alphareductase.csv : 20.10 %
allopregnanolone.csv : 5.83 %
srd5a3.csv : 4.76 %
dht.csv : 4.32 %
dihydroprogesterone.csv : 2.10 %
testosterone_production.csv : 0.65 %
p450scc.csv : 0.60 %
isotretinoin.csv : 0.27 %
hydroxysteroid_dehydrogenase.csv : 0.26 %
pregnenolone.csv : 0.22 %
star.csv : 0.19 %
3betahsd.csv : 0.19 %
selenium.csv : 0.17 %
steroidogenesis_human.csv : 0.17 %
human_semen.csv : 0.11 %
curcumin.csv : 0.10 %
adrenergic_receptor.csv : 0.10 %
cyp3a4.csv : 0.09 %
excitotoxicity.csv : 0.07 %
butyrate.csv : 0.06 %
adrenal_hyperplasia.csv : 0.06 %
hpa_axis.csv : 0.06 %
p450oxidoreductase.csv : 0.05 %
selenium_deficiency.csv : 0.05 %
udpgluc.csv : 0.05 %
nadph_human.csv : 0.04 %
gaba_human.csv : 0.04 %
anhedonia.csv : 0.04 %
cortisol_levels.csv : 0.04 %
cyp2d6.csv : 0.03 %
endothelial_nos.csv : 0.03 %
orthostatic_intolerance.csv : 0.03 %
resveratrol.csv : 0.03 %
cyp1a1.csv : 0.03 %
mcp-1.csv : 0.02 %
vitamin_d3.csv : 0.02 %
hmgcoa.csv : 0.02 %
cyp2e1.csv : 0.02 %
zinc_supplementation.csv : 0.02 %
caspase_human.csv : 0.02 %
hepatotoxicity.csv : 0.02 %
dopamine_levels.csv : 0.02 %
serotonin_levels.csv : 0.02 %
insulin_resistance.csv : 0.02 %
cox-2.csv : 0.02 %
cimetidine.csv : 0.02 %
limbic_system.csv : 0.02 %
omega3.csv : 0.01 %
rituximab.csv : 0.01 %
dopamine.csv : 0.01 %
inducible_nos.csv : 0.01 %
histone_deacetylase.csv : 0.01 %
l-dopa.csv : 0.01 %
social_anxiety.csv : 0.01 %
nafld.csv : 0.01 %
neurite_outgrowth.csv : 0.01 %
er_stress.csv : 0.01 %
oxalates.csv : 0.01 %
tau.csv : 0.01 %
inflammatory_response.csv : 0.01 %
l_carnitine.csv : 0.01 %
steatohepatitis.csv : 0.01 %
adrenal_insufficiency.csv : 0.01 %
dysautonomia.csv : 0.01 %
oxidative_stress_markers.csv : 0.01 %
taurine.csv : 0.01 %
glycoproteins.csv : 0.01 %
nadh_human.csv : 0.01 %
hsp70.csv : 0.01 %

I have not included entries with 0% hits

 

[mariovitali]

@Hip @JPV @Ema @Violeta @JaimeS

FYI : Cannabidiol reduces ER Stress :

Cannabidiol protects oligodendrocyte progenitor cells from inflammation-induced apoptosis by attenuating endoplasmic reticulum stress

Cannabidiol (CBD) is the most abundant cannabinoid in Cannabis sativathat has no psychoactive properties. CBD has been approved to treat inflammation, pain and spasticity associated with multiple sclerosis (MS), of which demyelination and oligodendrocyte loss are hallmarks. Thus, we investigated the protective effects of CBD against the damage to oligodendrocyte progenitor cells (OPCs) mediated by the immune system. Doses of 1μM CBD protect OPCs from oxidative stress by decreasing the production of reactive oxygen species. CBD also protects OPCs from apoptosis induced by LPS/IFNγ through the decrease of caspase 3 induction via mechanisms that do not involve CB1, CB2, TRPV1 or PPARγ receptors. Tunicamycin-induced OPC death was attenuated by CBD, suggesting a role of endoplasmic reticulum (ER) stress in the mode of action of CBD. This protection against ER stress-induced apoptosis was associated with reduced phosphorylation of eiF2α, one of the initiators of the ER stress pathway. Indeed, CBD diminished the phosphorylation of PKR and eiF2αinduced by LPS/IFNγ. The pro-survival effects of CBD in OPCs were accompanied by decreases in the expression of ER apoptotic effectors (CHOP, Bax and caspase 12), and increased expression of the anti-apoptotic Bcl-2. These findings suggest that attenuation of the ER stress pathway is involved in the ‘oligoprotective’ effects of CBD during inflammation.

You can also search on how Cannabis has helped a lot of Member forums. Here is one thread :


http://forums.phoenixrising.me/inde...ve-helped-me-significantly.32007/#post-492971


An Accutane sufferer had complete remission of symptoms using Cannabis :


http://www.allthingsmale.com/community/threads/recovery-story.24139/

 

[Violeta]

Are you able to get cannabidiol?

The type of ER stress caused by infiltration of viruses is what I am trying to figure out. When a virus has replicated all over your body how on earth to you get your body free of it if it's in the endoplasmic reticulum?

If ER stress caused by a drug can be relieved, and the remains of the drug removed, you will probably be more likely to retain your health improvement more easily than if there is a virus causing the stress. You may be very fortunate, hoping the best for you.

But I feel very fortunate that you brought your information here for expansion into the pathogen induced syndrome.

I think this is what causes the chronic fatigue syndrome. I am looking for an article that would explain how viruses in the ER cause lack of energy.

Do you know how ER stress causes OI? Maybe through neurodegeneration? Although it seems more easily reversible than something caused by neurodegeneration. That's another question that's in my mind this morning, and I will be looking around, but if you already have some specific information about that, I would appreciate it.

It seems as though ER stress is a cause of genetic mutations, have you found that conclusion in your reading?

I'm also wondering if since choline is an important remedial supplement for ER stress, and likewise for fatty liver, if the ER specifically has anything to do with fatty liver.

Maybe all these questions are already answered in this thread but I don't realize because when you don't know much about a subject you don't retain much of what you read. I'll go through the thread again. Your program generated lists will probably mean more to me this time around.

Another in depth educational article about endoplasmic reticulum.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3666557/

 

[mariovitali]

@Valentijn


I had a look at the rs643788 you provided as an example. dbSNP does list this Gene as benign. So far so good.

Then i look at the relevant SNPedia entry :

...and it lists 'C;C' (which i have given my 23andme results) as 'Risk' under 'ClinVar' section. Notice also that under 'Venter snp' section it says that defects in DPAGT1 are the cause of congenital disorder of glycosylation type lj. Despite this entry, the Significance is "Non-pathogenic"


So what am i missing here?

 

[Violeta]

I found this as a result of reading in the current selenium thread.

The selenium thread would therefore be showing pertinent information for selenium and B2 with respect to ER.

http://www.febsletters.org/article/0014-5793(94)01386-F/abstract

 

[mariovitali]

@Violeta


Here is a run for thioredoxin reductase. In bold i have some potentially interesting matched Topics :

*********Topic : thioredoxin reductase ***************
selenium_deficiency.csv : 2.91 %
selenium.csv : 1.57 %
nadph_human.csv : 1.30 %
oxidative_stress_protection.csv : 0.81 %
lipoic_acid.csv : 0.79 %
ros.csv : 0.43 %
nadh_human.csv : 0.41 %
peroxynitrite.csv : 0.37 %
tetrahydrobiopterin.csv : 0.28 %
oxidative_stress_markers.csv : 0.28 %
curcumin.csv : 0.27 %
pqq.csv : 0.25 %
gtp_cyclohydrolase.csv : 0.16 %
cyp1b1.csv : 0.16 %
upr.csv : 0.16 %
l_tyrosine.csv : 0.15 %
udpgluc.csv : 0.14 %
isotretinoin.csv : 0.13 %
er_stress.csv : 0.13 %
mitochondrial_dysfunction.csv : 0.12 %
atf6.csv : 0.11 %
chaperones.csv : 0.11 %
coenzymeq10.csv : 0.11 %
hsp70.csv : 0.10 %
caspase_human.csv : 0.10 %
cyp1a1.csv : 0.09 %
heat_shock_protein.csv : 0.09 %
cyp1a2.csv : 0.08 %
gpr78.csv : 0.08 %
endothelial_nos.csv : 0.08 %
pxr.csv : 0.08 %
rar.csv : 0.08 %
hepatotoxicity.csv : 0.07 %
xbp1.csv : 0.07 %
p53.csv : 0.06 %
misfolded_proteins.csv : 0.06 %
dolichol.csv : 0.06 %
sirt1.csv : 0.05 %
p5p.csv : 0.05 %
urea_cycle.csv : 0.05 %
caloric_restriction.csv : 0.05 %
hepatocytes.csv : 0.05 %
freet3.csv : 0.05 %
advanced_glycation_end.csv : 0.05 %
vitamin_k2.csv : 0.05 %
p450oxidoreductase.csv : 0.04 %
histone_deacetylase.csv : 0.04 %
resveratrol.csv : 0.04 %
glutamate.csv : 0.04 %
l_carnitine.csv : 0.04 %
perk.csv : 0.03 %
calcium_homeostasis.csv : 0.03 %
triiodothyronine_levels.csv : 0.03 %
taurine.csv : 0.03 %
pbmc.csv : 0.03 %
phospholipid_human.csv : 0.03 %
rxr.csv : 0.03 %
vcam-1.csv : 0.03 %
inducible_nos.csv : 0.03 %
monosodium_glutamate.csv : 0.03 %
cox-2.csv : 0.03 %
neuronal_nos.csv : 0.03 %
inflammatory_response.csv : 0.02 %
trpv.csv : 0.02 %
zinc_supplementation.csv : 0.02 %
vitamin_d3.csv : 0.02 %
tau.csv : 0.02 %
steatohepatitis.csv : 0.02 %
dopamine_levels.csv : 0.02 %
acetyl-coa.csv : 0.02 %
pregnenolone.csv : 0.01 %
uric_acid.csv : 0.01 %
inositol.csv : 0.01 %
nafld.csv : 0.01 %
glycosylation.csv : 0.01 %
amyloid.csv : 0.01 %
iron_deficiency.csv : 0.01 %
sshl.csv : 0.01 %
hmgcoa.csv : 0.01 %
immune_response.csv : 0.01 %
ebv.csv : 0.01 %
tinnitus.csv : 0.01 %
l-arginine.csv : 0.01 %
ngf.csv : 0.01 %
dopamine.csv : 0.01 %
mcp-1.csv : 0.01 %
probiotics.csv : 0.01 %
phosphatidylcholine.csv : 0.01 %
insulin_resistance.csv : 0.01 %
adrenal_insufficiency.csv : 0.01 %
cholestasis.csv : 0.01 %
human_proteinuria.csv : 0.01 %
human_semen.csv : 0.01 %
gluten.csv : 0.00 %
microglia.csv : 0.00 %
omega3.csv : 0.00 %
l-dopa.csv : 0.00 %
cortisol_levels.csv : 0.00 %
autism.csv : 0.00 %
ckd.csv : 0.00 %
tudca.csv : 0.00 %
choline_deficiency.csv : 0.00 %
insomnia.csv : 0.00 %
5-htp.csv : 0.00 %
adrenal_hyperplasia.csv : 0.00 %
mastocytosis.csv : 0.00 %
scfa.csv : 0.00 %
p450scc.csv : 0.00 %
limbic_system.csv : 0.00 %
il_10.csv : 0.00 %
5mthf.csv : 0.00 %
panic_disorder.csv : 0.00 %
adhd.csv : 0.00 %
dpagt1.csv : 0.00 %
stat1.csv : 0.00 %
pgc1.csv : 0.00 %
dht.csv : 0.00 %
hmgb1.csv : 0.00 %
n-acetylglucosamine.csv : 0.00 %
phenylketonuria.csv : 0.00 %
irritable_bowel.csv : 0.00 %
5alphareductase.csv : 0.00 %
ire1.csv : 0.00 %
3betahsd.csv : 0.00 %
nlinkedglycosylation.csv : 0.00 %
mast_cell_activation.csv : 0.00 %
insp3.csv : 0.00 %
gaba_human.csv : 0.00 %
orthostatic_intolerance.csv : 0.00 %
cerebrovascular_amyloidosis.csv : 0.00 %
butyrate.csv : 0.00 %
social_anxiety.csv : 0.00 %
systemic_amyloidosis.csv : 0.00 %
oxalates.csv : 0.00 %
microbiome_humans.csv : 0.00 %
udpglcnac.csv : 0.00 %
hydroxysteroid_dehydrogenase.csv : 0.00 %
finasteride.csv : 0.00 %
cfs.csv : 0.00 %
hexosamine.csv : 0.00 %
adrenergic_receptor.csv : 0.00 %
glycerylphosphorylcholine.csv : 0.00 %
benfotiamine.csv : 0.00 %
cimetidine.csv : 0.00 %
anhedonia.csv : 0.00 %
tocotrienol.csv : 0.00 %
cyp3a4.csv : 0.00 %
cyp2d6.csv : 0.00 %
fmo3.csv : 0.00 %
baroreceptor.csv : 0.00 %
o-glcnacylation.csv : 0.00 %
crohns_disease.csv : 0.00 %
protease_inhibitor.csv : 0.00 %
neurite_outgrowth.csv : 0.00 %
sinusitis.csv : 0.00 %
beta-alanine.csv : 0.00 %
testosterone_production.csv : 0.00 %
car.csv : 0.00 %
mucuna.csv : 0.00 %
vitamin_b6.csv : 0.00 %
dysautonomia.csv : 0.00 %
ginkgo.csv : 0.00 %
allopregnanolone.csv : 0.00 %
mthfr.csv : 0.00 %
amyloidosis.csv : 0.00 %
erad.csv : 0.00 %
l_tryptophan.csv : 0.00 %
creatine_supplementation.csv : 0.00 %
steroidogenesis_human.csv : 0.00 %
serotonin_levels.csv : 0.00 %
glycoproteins.csv : 0.00 %
dihydroprogesterone.csv : 0.00 %
cyp2e1.csv : 0.00 %
hsc.csv : 0.00 %
osmolytes.csv : 0.00 %
floaters.csv : 0.00 %
acetylcholine.csv : 0.00 %
asymmetric_dimethylarginine.csv : 0.00 %
rituximab.csv : 0.00 %
d-limonene.csv : 0.00 %
resistant_starch.csv : 0.00 %
excitotoxicity.csv : 0.00 %
hpa_axis.csv : 0.00 %
hgh.csv : 0.00 %
tmao.csv : 0.00 %
atrial_fibrillation.csv : 0.00 %
magnesium_deficiency.csv : 0.00 %
subclinicalhypo.csv : 0.00 %
o-glcnac.csv : 0.00 %
star.csv : 0.00 %
srd5a3.csv : 0.00 %

 

[Ema]

@Hip @JPV @Ema @Violeta @JaimeS

FYI : Cannabidiol reduces ER Stress :





You can also search on how Cannabis has helped a lot of Member forums. Here is one thread :


http://forums.phoenixrising.me/inde...ve-helped-me-significantly.32007/#post-492971


An Accutane sufferer had complete remission of symptoms using Cannabis :


http://www.allthingsmale.com/community/threads/recovery-story.24139/

What do you know? I just ordered some of this yesterday...

 

[ppodhajski]

@Violeta

Here is a run for thioredoxin reductase. In bold i have some potentially interesting matched Topics :

Very interested in what you are doing here. Can you explain it to me? Where are you getting this data?

 

[Violeta]

Quite an interesting list, @mariovitali !

 

[ppodhajski]

You might want to add FADS1 gene to your list of things effecting the endoplastic reticulum

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3540860/

and

http://datasheets.scbt.com/sc-292676.pdf
Members of the fatty acid desaturase (FADS) family, including FADS1, FADS2
and FADS3, regulate the desaturation of fatty acids by introducing double
bonds between defined carbons of fatty acyl chains, thereby playing an
essential role in the lipid metabolic pathway. Members of this family share
N-terminal cytochrome b5-like domains, C-terminal multiple membrane-
spanning desaturase regions and three histidine box motifs. It has been
suggested that single nucleotide polymorphisms (SNPs) within the FADS
gene cluster may be associated with diseases related to inflammation and
immunity processes. FADS1, also known as Delta-5 desaturase or D5D, is a
444 amino acid protein that is abundantly expressed in liver, brain, adrenal
gland and heart. Localized to the endoplasmic reticulum where it exists as
a multi-pass membrane protein, FADS1 catalyzes the biosynthesis of highly
unsaturated fatty acids from linoleic acid and α-linolenic acid. Additionally,
FADS1 functions to catalyze the desaturation of both dihomo-γ-linoleic acid
(DHGLA) and eicosatetraenoic acid (EA) to produce arachidonic acid (AA)
and eicosapentaenoic acid (EPA), respectively.

 

[mariovitali]

@Violeta

Yes and this is the reason for having Selenium in my regimen. While analyzing my personal data there was a clear pattern that Selenium was beneficial for my Symptoms. After looking how important Selenium is to proper Protein Folding then it all made sense.

I will make a full run again with my software, add selenoproteins and report back

 

[Violeta]

I think I'll get the homeopathic form, too

http://www.homeoint.org/books/boericmm/s/sel.htm

Do think it's possible that a deficiency of selenium had a part in your hair loss to begin with?

 

[JaimeS]

Is cannabinol in hemp seed, I wonder?

http://www.davoil.ro/documente/the-...otential-as-an-important-source-nutrition.pdf

Yes, in trace amounts:

The presence of CBD is significant because it has documented anticonvulsive, anti-epileptic, and antimicrobial properties (Karler and Turkanis, 1973; Ferenczy, Gracza & Jakobey, 1958). Although the levels of CBD within the oil are typically small, many health benefits may still be gained from its presence.

Antimicrobial activity has also been reported for CBD. Specifically, CBD has been shown to inhibit the growth in Gram-positive bacteria such as Streptomyces griseus and Staphylococcus aureus (Ferenczy et al. 1958). These organisms are particularly sensitive to extracts of Cannabis in slightly acidic culture medium even at dilutions as low as 5 ppm.

LOL, one of my old professors wrote this one!

So, you want to try this? If so, I have a recipe for you! If I do a blog post on it, I will link from here.


-J

 

[Valentijn]

...and it lists 'C;C' (which i have given my 23andme results) as 'Risk' under 'ClinVar' section.

Without any link or explanation there, there seems to be no basis for it to be considered a risky version. I would mostly use SNPedia as an easy way to get to some of the research, and wouldn't really trust any summaries. Sort of like using Wikipedia to get a basic understanding, and find sources of reputable data

Notice also that under 'Venter snp' section it says that defects in DPAGT1 are the cause of congenital disorder of glycosylation type lj. Despite this entry, the Significance is "Non-pathogenic"

They're talking only about the gene. There are hundreds or thousands of SNPs on each gene, and usually only a couple dozen are known to cause serious problems. So the gene data cannot be extrapolated to the SNP, unless the SNP can cause the gene to malfunction.

But even in the absence of research into the effects of a SNP, the odds of it having an impact can be calculated, at least when it's a missense mutation. The simplest way is with a BLOSUM62 chart:

In the case of poor rs643788, which is now getting picked on awful lot , the substitution is between Ile and Val. Where the two amino acids intersect (on the bottom line), the value is 3. Basically the higher the value, the more similar the amino acids are and the less likely it is that substituting one for the other will cause problems. 3 is pretty high, so that strongly suggests that having isoleucine instead of valine isn't significant.

@JaimeS wrote some excellent blog posts on looking into these sorts of things, including some more in-depth bits. Her explanations tend to be more coherent than mine

 

[mariovitali]

OMG @Valentijn ...

OK i am completely at loss here haha


@JaimeS is it possible for you to let us know if the list of Genes + Risk alleles in the beginning of the post does indeed hold?

I thought i had a basic understanding of this information but it appears that i have none. Here is what i have on my notes. I found the risk alleles from this website https://www.pharmgkb.org/index.jsp where you can enter an rs# :

NAFLD , Choline deficiency

rs3733890(Risk A)
rs2461823 (Risk C) NAFLD Disease
rs7643645 (Risk G) NAFLD Disease
rs7946 (Risk T) (PEMT)
rs4244593 (Risk G) (PEMT)
rs2236225 (Risk A) (MTHFD1)
rs9001 (Risk G)(CHDH)


ER Stress response


rs13045 (EIF2AK3-PERK) : Risk C
rs2239815(XBP1) : Risk C
rs10918270(ATF6) : Risk A
rs391957 (HSPA5) : Risk C

DPAGT1 (Glycosylation)

rs643788(Risk C)
rs28934876 (Risk C)


GCH1, associated with lower levels of BH4


rs10483639 (Risk C)
rs3783641 (Risk A)
rs8007267 (Risk T)
rs12147422 (Risk C)
rs3783637 (Risk T)
rs3783641 (Risk A)
rs41298442 (Risk T)
rs4411417 (Risk C)
rs752688 (Risk T)
rs841 (Risk A)
rs998259 (Risk T)
rs7147286 (Risk A)


PAH Gene - Phenylalanine levels handling

rs10860936 (Risk C)
rs1722387 (Risk C)
rs1522305 (Risk C)
rs1522296 (Risk A)
rs772897 (Risk C)
rs1522307 (Risk G)
rs11111419 (Risk T)


I am sure that i've been through some research documents discussing how certain gene mutations lead to Choline deficiency and NAFLD.

I will find them ASAP.

 

[ppodhajski]

@Valentijn


I had a look at the rs643788 you provided as an example. dbSNP does list this Gene as benign. So far so good.

Then i look at the relevant SNPedia entry :


View attachment 12305



...and it lists 'C;C' (which i have given my 23andme results) as 'Risk' under 'ClinVar' section. Notice also that under 'Venter snp' section it says that defects in DPAGT1 are the cause of congenital disorder of glycosylation type lj. Despite this entry, the Significance is "Non-pathogenic"


So what am i missing here?

Non-pathogenic only means it does not cause disease ON ITS OWN. It does not mean that there is not a change in function of the protein or enzyme. It could mean that it is one gene in many that together are pathogenic.

Single genes and single SNPs are rarely important. Upstream and downstream genes, other genes it interacts with, and diet and environment as well also must be taken into account.