Tissue specific signature of HHV-6 infection in ME/CFS (Bhupesh Prusty)

sb4

sb4

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https://www.frontiersin.org/articles/10.3389/fmolb.2022.1044964/abstract
First exposure to various human herpesviruses (HHVs) including HHV-6, HCMV and EBV does not cause a life-threatening disease. In fact, most individuals are frequently unaware of their first exposure to such pathogens. These herpesviruses acquire lifelong latency in the human body where they show minimal genomic activity required for their survival. We hypothesized that it is not the latency itself but a timely, regionally restricted viral reactivation in a sub-set of host cells that plays a key role in disease development. HHV-6 (HHV-6A and HHV-6B) and HHV-7 are unique HHVs that acquire latency by integration of the viral genome into sub-telomeric region of human chromosomes. HHV-6 reactivation has been linked to Alzheimer’s Disease, Chronic Fatigue Syndrome, and many other diseases. However, lack of viral activity in commonly tested biological materials including blood or serum strongly suggests tissue specific localization of active HHV-6 genome. Here in this paper, we attempted to analyze active HHV-6 transcripts in postmortem tissue biopsies from a small cohort of ME/CFS patients and matched controls by fluorescence in situ hybridization using a probe against HHV-6 microRNA (miRNA), miR-aU14. Our results show abundant viral miRNA in various regions of the human brain and associated neuronal tissues including the spinal cord that is only detected in ME/CFS patients and not in controls. Our findings provide evidence of tissue-specific active HHV-6 and EBV infection in ME/CFS, which along with recent work demonstrating a possible relationship between herpesvirus infection and ME/CFS, provide grounds for renewed discussion on the role of herpesviruses in ME/CFS.
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BrightCandle

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This would explain why Ampligen has some effect, why some people seem to recover on Valcyte and other immune modulating drugs. It was also explain the metabolic shift and Itacontate shunt and even the microclots but also why we don't see it in the blood. This is very useful research that I want a replication of from the existing brain banks, should be easy enough to replicate.

It also is a route that has treatments already, quite a lot of them infact to try all be it all of them prescription required.
 
H

Husband of

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From bhupesh prusty on twitter

". This was once in a life time opportunity for us to test 3 patients. We found HHV-6 miR-aU14 in 2. But all 3 had EBV. We tested 26 nonME/CFS samples and all were negative within specific tissues."
 
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Marylib

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I guess we should be happy about more autopsies? I feel like what Hillary said about it. It's not like it's a big mystery. It's just that no one cares to look. But yes, grateful that Bhupesh scraped together enough funding for this.

"Dear NIH, you really blew it. Prusty studies post mortem brain samples to find destructive virus HHV6 reactivated throughout central nervous system in ME but not controls."

https://twitter.com/i/web/status/1599799390658768897
 
T

Tom Kindlon

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Husband of said:
From bhupesh prusty on twitter

". This was once in a life time opportunity for us to test 3 patients. We found HHV-6 miR-aU14 in 2. But all 3 had EBV. We tested 26 nonME/CFS samples and all were negative within specific tissues."
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Preliminary work was done on the feasibility of a ME/CFS post-mortem brain and tissue bank in the UK

See:
Exploring the feasibility of establishing a disease-specific post-mortem tissue bank in the UK: a case study in ME/CFS
https://jcp.bmj.com/content/63/11/1032
&
Considerations in establishing a post-mortem brain and tissue bank for the study of myalgic encephalomyelitis/chronic fatigue syndrome: a proposed protocol
https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-7-370

Unfortunately there wasn't enough private funding around.

Hopefully people can make it happen.
 
godlovesatrier

godlovesatrier

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Bhupesh responded in the thread saying that antivirals won't work, and then he sort of lost me with the rest of the explanation. I haven't got a link on my laptop but maybe some other kind soul can link it.
 
Cort

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Exciting stuff....Looking forward to the full paper.

I wonder where he got his samples from _ I heard a bank was opening in Stanford but wasn't sure. Maybe this will give that bank proposal a boost.

The patients were so severe that they died - so we don't know if the same findings are present up in less severe patients. I wonder he can draw a straight line from the HHV-6 infection to their deaths and what his next step is?
 
Rufous McKinney

Rufous McKinney

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If a disease is largely not diagnosed, how do autopsy samples exist for that disease?

I wonder who generates a death certificate stating: Died of ME.

Given that people may die of many things, and ALSO have ME; seems like there are many many autopies that might in fact show data for viruses in tissue. If we looked.
 
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