Jumping in with my 23andMe results for comment...

Critterina

Critterina

Senior Member
Messages
1,238
Location
Arizona, USA
I have been on this website for almost a month now and have really grown to respect the knowledge and appreciate the support here. My next appointment is Tuesday, and I realize that none of my health care providers have experience with anything more than MTHFR C677T and A1298C. So here's my results from Genetic Genie, comments welcome.
Methylation:
VDR Bsm rs1544410 TT +/+
MAO A R297R rs6323 GT +/-
MTHFR 03 P39P rs2066470 AG +/-
MTHFR A1298C rs1801131 GT +/-
MTR A2756G rs1805087 AG +/-
MTRR A66G rs1801394 GG +/+
MTRR A664A rs1802059 AG +/-
BHMT-08 rs651852 TT +/+
CBS C699T rs234706 AG +/-
CBS A360A rs1801181 AG +/-
SHMT1 C1420T rs1979277 AA +/+
and Detox:
CYP1A2 164A>C rs762551 AC +/-
CYP1B1 L432V rs1056836 CG +/-
CYP1B1 R48G rs10012 CG +/-
CYP2C9*2 C430T rs1799853 CT +/-
CYP2D6 S486T rs1135840 -- no call
CYP2D6 100C>T rs1065852 AG +/-
CYP2D6 2850C>T rs16947 AG +/-
GSTP1 I105V rs1695 AG +/-
NAT2 I114T rs1801280 CT +/-
NAT2 K268R rs1208 AG +/-
and Rare MTHFR Alleles:
rs3737967....R492H...G....T is protectiveGG
rs4846049....A372C...T GT
rs1476413....G35A....T CT
rs1801131....A1298C..G GT
and Slow COMT:
rs165722.....C201T...C.......Implied CC


Thank you!

EDIT: and these results just in:
Other Slow VDR:

rs11574115...T362I...G.A....GG..... +/+ -/- (Edited to correct. This one shouldn't be in the list.)
rs731236.....Taq1....G.....GG..... +/+
rs7975232....Apa1....A.....AA..... +/+
rs1544410....Bsm1....T.....TT..... +/+
rs2239179....A1064G..T.....TT..... +/+
rs886441.....C4004T..A.....AA..... +/+
rs3819545....T6046C..G.....GG..... +/+
rs11168287...C8857T..GG.....GG..... +/+
rs7139166....G1520C..CC.....CC..... +/+
 
V

Valentijn

Senior Member
Messages
15,786
Critterina said:
Methylation:
VDR Bsm rs1544410 TT +/+
MAO A R297R rs6323 GT +/-
MTHFR 03 P39P rs2066470 AG +/-
MTHFR A1298C rs1801131 GT +/-
MTR A2756G rs1805087 AG +/-
MTRR A66G rs1801394 GG +/+
MTRR A664A rs1802059 AG +/-
BHMT-08 rs651852 TT +/+
CBS C699T rs234706 AG +/-
CBS A360A rs1801181 AG +/-
SHMT1 C1420T rs1979277 AA +/+
and Rare MTHFR Alleles:
rs3737967....R492H...G....T is protectiveGG
rs4846049....A372C...T GT
rs1476413....G35A....T CT
rs1801131....A1298C..G GT
and Slow COMT:
rs165722.....C201T...C.......Implied CC
Click to expand...
You're lacking any of the really nasty MTHFR variations, but you do have quite a few of the milder ones, so it certainly seems possible that things are somewhat slow there in producing methylfolate. SHMT might be interacting with those to make them worse, but that's speculation based on a known interaction between SHMT and MTHFR C677T, which you don't have.

Your MTR/MTRR might also be quite problematic. According to http://www.ncbi.nlm.nih.gov/pubmed/12416982 , your version of A66G (I22M) can result in a 3-4 fold increase in the amount of MTRR (methionine synthase reductase) needed to recycle MTR (methionine synthase). Hence you might need to supplement a lot of B12 to help keep things moving there. Heterozygous A664A probably isn't having an impact.

With your BHMT-08 and CBS C699T you might be prone to accumulating homocysteine. B6 can help in getting rid of that via the CBS route. A normal dose is probably plenty, especially since higher doses can be toxic.

Since you have some slow versions of VDR, COMT, and MAOA, methyl groups might (or might not) cause you problems. If going with a high dose of B12, hydroxoB12 might be the safer one to try, or it could be a good idea to be cautious if increasing methylB12 supplementation.

SUMMARY:
Normal doses of B6 and methylfolate are probably a good idea, and B12 supplementation is strongly indicated as well.
 
Critterina

Critterina

Senior Member
Messages
1,238
Location
Arizona, USA
Valentijn said:
You're lacking any of the really nasty MTHFR variations, but you do have quite a few of the milder ones, so it certainly seems possible that things are somewhat slow there in producing methylfolate. SHMT might be interacting with those to make them worse, but that's speculation based on a known interaction between SHMT and MTHFR C677T, which you don't have.

Your MTR/MTRR might also be quite problematic. According to http://www.ncbi.nlm.nih.gov/pubmed/12416982 , your version of A66G (I22M) can result in a 3-4 fold increase in the amount of MTRR (methionine synthase reductase) needed to recycle MTR (methionine synthase). Hence you might need to supplement a lot of B12 to help keep things moving there. Heterozygous A664A probably isn't having an impact.

With your BHMT-08 and CBS C699T you might be prone to accumulating homocysteine. B6 can help in getting rid of that via the CBS route. A normal dose is probably plenty, especially since higher doses can be toxic.

Since you have some slow versions of VDR, COMT, and MAOA, methyl groups might (or might not) cause you problems. If going with a high dose of B12, hydroxoB12 might be the safer one to try, or it could be a good idea to be cautious if increasing methylB12 supplementation.

SUMMARY:
Normal doses of B6 and methylfolate are probably a good idea, and B12 supplementation is strongly indicated as well.
Click to expand...
Thank you so much, Valentijn! :thumbsup:

I started down this path when I had respiratory symtoms (nasal blockage, 80% lung capacity) that remained post-upper respiratory infection. After 10 months of breathing meds, I found some of it was diet-related, so I sought help.

I had labs done in January. I had been taking the Carlson mini-multivitamin, 6000 IU of D3 and 100 mg of B6 (inactive) for several months before the test. I was put on 1 mg MTHF, 5 mg MB12, and 100 mg P5P, (Are those normal doses?) plus a bunch of antioxidants and branch chain amino acids for muscle wasting. And low dose thyroid because of low morning temps and high TSH.

One day I did a test and took 3 of the 5 mg MB12. No reaction, so I figured I wasn't sensitive to methyl groups. I use Kirkland Signature MB12.

After 3 months, We tested homocysteine on my request, and it was not high (19% of the normal range), but methionine was just below normal, too. I wonder if Me were normal, would more homocysteine accumulate? Tryptophan was also low, so, 500 mg Try, 1500 mg Me, and 2000 mg NAC were added to my protocol, (and more thyroid, hormone support, and bovine adrenal.) The MTHF, MB12, and P5P doses were unchanged.

One day I took 500 mg TMG as an experiment, to try to recycle my homocysteing, being my Me was low. I sat down and played the piano as if I were angry with it, or perhaps agitated. (My teacher is always trying to get me to play louder.) I was thinking it would supply BHMT support, now that I wasn't living on high-betaine foods, like I had in the winter (mostly quinoa and beets). I haven't repeated that test yet, but perhaps that would indicate some methyl group sensitivity.

After 3 more weeks I got "Level 1 folate insufficiency" per Freddd 's description in "The Stages of Methylation and Healing" thread. But I hadn't read the thread at that point, so I just lived with it. I stuck with my protocol until the next lab test, which happened about the same time I got my 23andMe results. Then I started to experiment.

First, I did one week with a low sulfur diet, no sulfur-containing supplements, 2 mg of MTHF and 800mg folinic acid. And I quit taking MTHF at the same time as my high dose Vitamin C. My level 1 symptoms were gone in 4 days and I lost almost 7 lbs of stomach bloat. It seemed the folinic acid made me sleepy after the first day, so I added L-carnitine, D-ribose, and a little NADH - and I didn't feel sleepy after taking it anymore.

Then I went back to a "regular" diet, with animal protein, grains, and such, increased the MTHF to 3 mg and dropped the folinic acid. My level 1 symptoms returned. After several days, I added back the folinic acid, added 10 mg AdenosylB12, and slowly increased the MTHF to 10 mg. Still some symptoms remained, and then my scalp broke out again. So I did 2 days of 30 mg MTHF, with only marginal improvement. I don't think I had any methly-block symptoms, but I'm not sure exactly what to look for except 'feeling terrible' and 'crashing'.

I received my labs and will talk with my practitioner tomorrow. The amino acid profile is whacked. I think I can explain most of it due to the high doses of NAC. Also, they urine copper test couldn't be performed because the concentration of the analyte was too low to detect. (Hmmm...no copper to support the DAO enzymes, plus taking high-histamine NAC three times a day...reacting to tomatoes, spinach, peppers with breathing problems...it all came together when I read about histamine intolerance! I never would have put this all together without the help and support of the people on this website!)

Perhaps I should experiment with holding the MTHF at some 'normal' level (5 mg?) and increasing the MB12 or combo of that and HydroxoB12 until my Level 1 goes completely away. Perhaps my Kirkland Signature MB12 is not the gold standard in MB12 and I need a better brand. I have a better idea now about what I want to get out of my appointment tomorrow, but I will certainly take more comments now that I've given some history. Thanks!
 
S

sregan

Senior Member
Messages
703
Location
Southeast
Critterina said:
Perhaps I should experiment with holding the MTHF at some 'normal' level (5 mg?) and increasing the MB12 or combo of that and HydroxoB12 until my Level 1 goes completely away. Perhaps my Kirkland Signature MB12 is not the gold standard in MB12 and I need a better brand. I have a better idea now about what I want to get out of my appointment tomorrow, but I will certainly take more comments now that I've given some history. Thanks!
Click to expand...

You should get the Enzymatic brand just to be sure. There a ton of posts here regarding B12 potency.
 
Critterina

Critterina

Senior Member
Messages
1,238
Location
Arizona, USA
sregan said:
http://www.iherb.com/Enzymatic-Therapy-B12-Infusion-Energy-30-Chewable-Tablets/2119

I believe this is it
Click to expand...
Thanks. Too bad I already have such a large supply. Well, if mine's not as potent, I guess I can get the same result by taking more, until I'm ready to resupply. I'll bookmark this page, though, thanks! :thumbsup:

Edit: :D Where's the guy rolling on the floor? I need him! When the page loaded, I saw that I have that kind too. I needed about $4 more on my order to get free shipping and it was something like $6. What a deal!

So do you chew them as directed, or try to let them dissolve over hours in the side of your mouth?
 
S

sregan

Senior Member
Messages
703
Location
Southeast
Critterina said:
Thanks. Too bad I already have such a large supply. Well, if mine's not as potent, I guess I can get the same result by taking more, until I'm ready to resupply. I'll bookmark this page, though, thanks! :thumbsup:

Edit: :D Where's the guy rolling on the floor? I need him! When the page loaded, I saw that I have that kind too. I needed about $4 more on my order to get free shipping and it was something like $6. What a deal!

So do you chew them as directed, or try to let them dissolve over hours in the side of your mouth?
Click to expand...

See this post: http://forums.phoenixrising.me/index.php?threads/b-12-the-hidden-story.142/page-138#post-338667


"Enzymatic Therapy B12 infusion and holding the tablet(s) under the lip for 45-120"

If you can tuck it under your upper lip and let it dissolve over 30+ minutes you'll get better absorption. The longer seems to be the better.

It's actually the first post in this forum and has 130+ pages of response it's been such an issue.
 
Critterina

Critterina

Senior Member
Messages
1,238
Location
Arizona, USA
Asking for more help here:

I was looking at someone's output from Sterling's analysis on MTHFR, and I decided to look at my results for the same SNPs. There were a couple of clusters that I wondered about:


Gene Variation rsID Risk Allele Your Alleles Result
ATP Synthase
ATP5c1 rs2778475 A AG +/-
ATP5c1 rs1244414 T CT +/-
ATP5c1 rs1244422 T TT +/+
ATP5c1 rs4655 C CC +/+
NADH Dehydrogenase
NDUFS7 rs2332496 A AG +/-
NDUFS7 rs7254913 G AG +/-
NDUFS7 rs1142530 T CT +/-
NDUFS7 rs7258846 T GT +/-
NDUFS7 rs11666067 A AC +/-
NDUFS7 rs2074895 A AC +/-
NDUFS7 rs809359 G AG +/-
Nitric Oxide Synthase 3
NOS3 rs1800783 A AA +/+
NOS3 rs1800779 G GG +/+
NOS3 G10T rs7830 T GT +/-
NOS3 T786C rs2070744 C CC +/+
Phosphatidylethanolamine N-methyltransferase
PEMT rs4244593 T GT +/-
PEMT rs4646406 A AT +/-
PEMT rs7946 C CT +/-
IgE
FCER1A rs2427837 A AG +/-
IL-13 C1112T rs1800925 T CT +/-
IL13 rs1295685 A AG +/-
SOCS-1 -820G>T rs33977706 A AC +/-
FCER1A/OR10J2P rs2494262 A AC +/-
FCER1A rs2251746 C CT +/-
RAD50 rs2040704 G AG +/-
RAD50 rs2240032 T CT +/-
IgG
FCGR2A rs1801274 A AA +/+
GSTM3 V224I rs7483 T CT +/-
IgA
TRAF1 rs3761847 G AG +/-
IRF5 rs4728142 A AG +/-
IGF1R rs2229765 A AA +/+
CFH rs6677604 A AG +/-
PSMB8/TAP1/TAP2 rs9357155 A AG +/-

Any help understanding how I might compensate for these would be helpful.
 
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