Positive in that dbSNP orientation is plus. So if dbSNP DOES correlate to positive for that SNP, then I have serious problems in GSTM1. If I crossreference my above posted results with the dbSNP orientation field, it seems they DO correlate. The field will be plus if you are either homozygous or herterozygous positive for the SNP.
I do hope my interpretation is rock solid, as I'm going to take these results to the doctor with me on Friday. It certainly sheds some light on why I have such a horrendous time supplementing almost anything in relation to the methylation cycle. I'm one of these people whose body rejects almost anything you throw at it.
The annoying thing is, recommendations for the 2 COMT homozygous positive SNPs I have is to supplement hydroxocobalamin -- but -- I suspect my glutathione is likely way too low for the enzyme that activates hydroxocobalamin to methylcobalamin to work, therefore this is kind of useless, but supplementing methylcobalamin will overload me with methyl groups.
Such a painful network in interrelated nightmares.
Hi hixxy,
I'm curious to see your GSTM1 results. Here are mine ("normal" call in square brackets):
GSTM1 -/- (CC)[CC] rs12068997
GSTM1 +/+ (GG)[AA] rs4147565
GSTM1 -/- (AA)[AA] rs4147567
GSTM1 +/+ (TT)[AA] rs4147568
GSTM1 -/- (CC)[CC] rs1056806
GSTM1 +/+ (TT)[AA] rs12562055
GSTM1 +/+ (AA)[GG] rs2239892
A lot of positives, too, like you mentioned, but such a grouping may simply represent a haplotype. Perhaps someone knows.
I had a MetaMetrix panel done in 2000. For detoxification of acetaminophen, the glutathione conjugation was only 50% of the lowest normal reference value ("acetaminophen mercapturate" was measured). I'm not sure how much influence GSTM1 has relative to the other glutathione-S-transferases in that particular case.
For anyone else interested, here is a recent (2011) listing of genes for the "glutathione pathway": GSH synthesis (GSS, GCLC, and GCLM); GSH redox status (GSR, GPX1, GPX2, GPX3, GPX4, GPX5, GPX6, and GPX7); GSH S-transferases (GSTA1, GSTA2, GSTA3, GSTA4, GSTA5, GSTM1, GSTM2, GSTM3, GSTM4, GSTM5, GSTO1, GSTO2, GSTP1, GSTT1, GSTT2, and GSTZ1); and GSH conjugate transporters (ABCC1, ABCC2, ABCC3, and ABCC4). From:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044203/
(BTW, hixxy, 23andMe is reporting VDR Taq with the call letters A and G in my report. You have CC . . .?)