urbantravels
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More detail on the 2008 prostate cancer study showing proviral integration that Eric Silverman of the Cleveland Clinic had referred to in the comments on an earlier posting.
http://www.virology.ws/2011/01/04/retroviral-integration-and-the-xmrv-provirus/
http://www.virology.ws/2011/01/04/retroviral-integration-and-the-xmrv-provirus/
A strong argument that the novel human retrovirus XMRV is not a laboratory contaminant is the the finding that viral DNA is integrated in chromosomal DNA of prostate tumors. Why does this result constitute such strong proof of viral infection?
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To identify XMRV proviral DNA, genomic DNA was isolated from prostate tumors, and DNA was amplified using a primer that annealed in the viral env gene, near the right-hand LTR. Nucleotide sequence analyses of amplified DNAs from 14 different patients showed the expected viral CA sequence followed by human DNA. However, the other cardinal sign of retroviral integration duplication of host DNA sequences flanking the integration site could not be confirmed, because only the right-hand integration site was studied.
The isolation of the entire proviral DNA, including both flanking integration sites, from patients with prostate cancer or chronic fatigue syndrome would be additional evidence that XMRV is a virus that infects humans.