I think its great that we can all pool our results together, in this new and relatively unexpolored/misunderstood field of nutritional genomics, to reach practical understandings that could make a huge difference in the quality of life for many here and those who will come searching for vital solutions in the future.
My 23&Me results for MTHFS:
MTHFS, ST20-MTHFS 80136129 rs685487 A or G AG
MTHFS, ST20-MTHFS 80136288 rs8033649 A or C CC
MTHFS, ST20-MTHFS 80137560 rs8923 C or T TT
MTHFS, ST20-MTHFS 80138571 rs2733103 C or T CC
MTHFS, ST20-MTHFS 80138745 rs16971427 A or C AA
MTHFS, ST20-MTHFS 80141997 rs655473 A or G AA
MTHFS, ST20-MTHFS 80144197 rs17284990 C or T CT
MTHFS, ST20-MTHFS 80154571 rs16971450 A or G AA
MTHFS, ST20-MTHFS 80154982 rs6495446 C or T CC
MTHFS, ST20-MTHFS 80158042
rs7177659 A or C AC
MTHFS, ST20-MTHFS 80158159 rs6495449 A or G GG
MTHFS, ST20-MTHFS 80162265 rs17285431 A or C AA
MTHFS, ST20-MTHFS 80164053 rs6495451 C or T CT
MTHFS, ST20-MTHFS 80165368 rs2586154 A or G GG
MTHFS, ST20-MTHFS 80168282 rs12899781 G or T TT
MTHFS, ST20-MTHFS 80171971 rs16971478 A or G AA
MTHFS, ST20-MTHFS 80172133 rs2586153 C or T CC
MTHFS, ST20-MTHFS 80174388 rs2562744 A or C AC
MTHFS, ST20-MTHFS 80177687 rs2733106 A or G AA
MTHFS, ST20-MTHFS 80178283 rs12438477 A or C AC
MTHFS, ST20-MTHFS 80182050 rs12898642 C or T CT
MTHFS, ST20-MTHFS 80183766 rs2586182 A or C AA
MTHFS, ST20-MTHFS 80184657 rs2733088 A or G AG
MTHFS, ST20-MTHFS 80186340 rs12440798 C or T CT
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I'm SHMT +/- with AG allele
Now I know these posts are only as useful as your rating of observations following folinic acid intake, but even after careful observation I don't know if I am or not. Before I attempt to explain my existing observations I should let you know what I take: For a week now I've been on my new methylation protocol involving deadlock quartet components except using ALCAR instead of LCF as I'm still waiting on the LCF to arrive in the mail. That is:
- Dr's Best Lithium Orotate (5mg/day since 8/11)
- Solgar Metafolin (800mcg/tablet) x 4-5 tablets spread over the day (and kept separate from Vitamin C and potassium intake)
- Enzyme Therapy sublingual MeB12 (2 x 1mg tablets in the morning and 1 in evening)
- Anabol Naturals Dibencozide (half the capsule (5mg ADB12) in the morning and the rest in the evening (another 5mg)
- Jarrow Sustain ALA (300mg x 2-3 times per day spread over the day)
- Co-Q10 (mix of old generic brand I'm running out of (150mg/tablet) and new for a total of 300mg Co-Q10/day
- Life Extension Foundation TMG (500mg) in the morning with the other components
- LEF P-5P (30mg) - 1/3rd of their 100mg capsule in the morning and repeated in evening.
- NOW Potassium gluconate (8 tablets a day spread over day and separate from the B12/Folate)
- Zinc amino acid chelate (2 x 22mg in morning and evening) with 1 chromium picolinate (400mcg) in evening.
General supps:
- 1 x 500mg Korean Ginseng tablet standardised to provide 15mg of ginsenosides in the morning
- 1 x Ginkgo Bilobo (6000mg - 28mg active) twice a day AM and PM
- Calcium Citrate (3 x 250mg/day)
-
2 x Saw Palmetto (each tablet standardised for 85% fatty sterols giving 128mg active ingredient) (ceased this as of morning of 10/11/13 to see if this has been the cause for my high fluid retention factor on a cellular health test and high Liver Function Test (AST in particular)
So, to simplify the above: I think i've got a comprehensive methylation protocol with no gaps whatsoever? and I may be experiencing some detox from the months of Saw Palmetto intake that I've been on in the leadup to this point (Apparently it can cause liver issues in rare cases)
So for two days now I've been feeling so tired in the morning that I've been having a 30-60min nap not long after breakfast which only involves half an apple, a chunk of freshly chopped pineapple, and 2 eggs with some mustard on top. I don't think this should contribute too much folinic acid (doesn't constitute a 'garden feast') as many term it.
So I'm guessing the tiredness is due to the strong upramping of the methylation cycle as a result of the adB12/MeB12/3mg MeFol/TMG/ALCAR/ALA taken in the morning.
For lunch I understand it should be the biggest meal of the day and so I have a meal based on a decent 200gm piece of meat with about 200gm of brussel sprouts and cabbage (steamed) which would supply an appreciable amount of dietary folates (I avoided saying folinic acid noting DBKita's useful post here:
http://forums.phoenixrising.me/inde...ients-plus-biomarker.21745/page-6#post-344781
I feel quite feeble after lunch, and one could be right in saying its because I've added all those dietary folates that my SHTM1+/- (AG) and MTHFS +/- (AC) is having trouble dealing with it resulting in folinic acid backcycle accumulation.. (thanks for this theory Nan Dixon) but after perusing this thread in great detail and some particularly pertinent comments from Lotus97 I realise that firstly we don't even know which allele of MTHFS or SHMT earnestly might cause a problem with dietary folates, and secondly, any symptoms which people are currently reporting as being 'folinic acid intolerance' might just as easily be overmethylation (which suggests the dietary folates are being processed just fine and so are contributing to the methylation cycle along with the existing methylfolates?)
Whilst on this point I just want to clarify my understanding of dietary folates to date because it seems to cause an awful lot of confusion as to how they're processed. DBKita suggests that vegetables contain a bunch of THF's that are packed into polyglutamates. Tying this in with Lotus97's input: the SHTM1 related enzyme does the first stage of processing the THF to a DHF and then MTHF677 does the rest through its relation to the DHFR enzyme which converts the Dihidrofolate to methylfolate? Is this correct? I seem to recall Dr Lynch (MTHFR.net fame) video which tied in the MTHF677 defect to the DHFR problem which results in unmetabolised folates floating around...which will be highly relevant for any with the hetero or homo mutations and if we can confirm for sure that vegetable folate conversion invariably involves the use of the DHFR enzyme...
I look forward to others contributing further to this thread so we can get some more clarity on this issue which has potentially important implications for all those looking to optimise diet and ensure their hard earned $$$ aren't being wasted on folate supplements...