G
Gerwyn
Guest
Elevated NO levels are a common finding in ME/CFS sufferers.A high nitric oxide level can Keep XMRV latent or hidden and or at a very low replicative titre .This is a common effect of NO where latent viruses are concerned. I was avirtual vegetable for four years untill someone gave me rediculously high doses of IV B12 which mops up No like a sponge.I was actually markedly better within four hours.totally anecdotal but it makes me wonder if XMRV if causative does its dirty work in its latent phase
Nitric Oxide Down-Regulates EpsteinBarr Virus Reactivation in Epithelial Cell Lines
Purchase the full-text article
References and further reading may be available for this article. To view references and further reading you must purchase this article.
Xiangrong Gaoa, Masako Tajimab and Takeshi Sairenjia, 1
a Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Nishimachi-86, Yonago, 683-8505, Japan
b Central Clinical Laboratory, Teikyo University School of Medicine, Tokyo, 173-8606, Japan
Received 7 December 1998;
revised 16 February 1999;
accepted 6 April 1999. ;
Available online 27 March 2002.
Abstract
Nitric oxide (NO), a mediator of biological functions, has an antimicrobial activity against a variety of pathogens including viruses. In this study, we found that a constitutive, low level of inducible NO synthase (iNOS) mRNA was expressed in the EBV-infected gastric tissue-derived GT38 and GT39 cell lines, by analysis with the reverse transcriptionpolymerase chain reaction (RTPCR) and Southern blotting. Treatment of these cells with a specific NOS inhibitor, NG-monomethyl-Image -arginine (Image -NMMA), induced the immediate-early, EBV transactivator gene BZLF1 protein ZEBRA, suggesting a significant increase in EBV reactivation by Image -NMMA. Northern blotting demonstrated that BZLF1 and BRLF1 transcripts were also induced by 12-O-tetradecanoylphorbol-13 acetate (TPA). Meanwhile, constitutive expression of iNOS mRNA was inhibited by TPA. Image -NMMA also enhanced TPA-induced expression of the BZLF1 gene. On the other hand, a NO donor, S-nitroso-N-acetylpenicillamine (SNAP), which releases NO in an aqueous solution, inhibited the TPA-induced BZLF1 gene expression in a dose-dependent manner at both mRNA and protein levels. These results demonstrated that NO is a regulatory factor in maintaining virus latency via inhibiting EBV reactivation in the infected epithelial cells.
Virology
Volume 258, Issue 2, 5 June 1999, Pages 375-381
Nitric Oxide Down-Regulates EpsteinBarr Virus Reactivation in Epithelial Cell Lines
Purchase the full-text article
References and further reading may be available for this article. To view references and further reading you must purchase this article.
Xiangrong Gaoa, Masako Tajimab and Takeshi Sairenjia, 1
a Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Nishimachi-86, Yonago, 683-8505, Japan
b Central Clinical Laboratory, Teikyo University School of Medicine, Tokyo, 173-8606, Japan
Received 7 December 1998;
revised 16 February 1999;
accepted 6 April 1999. ;
Available online 27 March 2002.
Abstract
Nitric oxide (NO), a mediator of biological functions, has an antimicrobial activity against a variety of pathogens including viruses. In this study, we found that a constitutive, low level of inducible NO synthase (iNOS) mRNA was expressed in the EBV-infected gastric tissue-derived GT38 and GT39 cell lines, by analysis with the reverse transcriptionpolymerase chain reaction (RTPCR) and Southern blotting. Treatment of these cells with a specific NOS inhibitor, NG-monomethyl-Image -arginine (Image -NMMA), induced the immediate-early, EBV transactivator gene BZLF1 protein ZEBRA, suggesting a significant increase in EBV reactivation by Image -NMMA. Northern blotting demonstrated that BZLF1 and BRLF1 transcripts were also induced by 12-O-tetradecanoylphorbol-13 acetate (TPA). Meanwhile, constitutive expression of iNOS mRNA was inhibited by TPA. Image -NMMA also enhanced TPA-induced expression of the BZLF1 gene. On the other hand, a NO donor, S-nitroso-N-acetylpenicillamine (SNAP), which releases NO in an aqueous solution, inhibited the TPA-induced BZLF1 gene expression in a dose-dependent manner at both mRNA and protein levels. These results demonstrated that NO is a regulatory factor in maintaining virus latency via inhibiting EBV reactivation in the infected epithelial cells.
Virology
Volume 258, Issue 2, 5 June 1999, Pages 375-381