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C-Reactive Protein Response in Patients With Post-Treatment Lyme Disease Symptoms Versus Those With Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Melanie Uhde,1 Alyssa Indart,1 Brian A. Fallon,2 Gary P. Wormser,3 Adriana R. Marques,4 Suzanne D. Vernon,5 and Armin Alaedini1
Departments of 1Medicine and 2Psychiatry, Columbia University Medical Center, New York, and 3Division of Infectious Diseases, Department of Medicine, New York Medical College, Valhalla; 4Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; and 5Bateman Horne Center, Salt Lake City, Utah
To the Editor—There is substantial overlap in symptoms, including fatigue, muscle and joint pain, and cognitive and memory deficits, between post-treatment Lyme disease syndrome (PTLDS) and myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) [1]. Increasing evidence suggests a role for immunologic and inflammatory pathways in both PTLDS and ME/CFS [2–4]. However, in part owing to their etiologic complexity and the lack of established biomarkers, our understanding of the pathways involved and potential mechanistic differences between the 2 conditions is very limited.
In a 2016 study published in Clinical Infectious Diseases, Uhde et al [5] examined the concentrations of acute-phase response proteins, including C-reactive protein (CRP), in individuals with PTLDS. CRP is a highly sensitive marker of infection and inflammation that binds a variety of ligands present on the surface of pathogens or exposed during autologous cell stress or death, exerting its effect through opsonin deposition and activation of the complement path- way, in addition to direct interaction with phagocytic cells [6]. We found that the circulating levels of CRP, as well as the frequency of concentrations >3 mg/ mL (generally considered to represent some degree of inflammation [7]) to be significantly higher in the PTLDS cohort than in a control group of subjects who had a history of Lyme disease but with- out persistent symptoms (both P < .001). The data provided evidence for increased expression of an objective marker of inflammation in PTLDS but suggested a mechanism of activation distinct from that in active infection, as previously dis- cussed [5].
Using the same methods [5] in a new study, we screened plasma samples from 131 patients with ME/CFS (89 female; mean age [standard deviation], 50.0 [11.4] years; mean body mass index (BMI), 26.0 [5.5]) and 86 healthy controls (68 female; mean age, 50.0 [12.8] years; mean BMI, 26.5 [6.8]), provided by the SolveCFS BioBank [8]. Patients with ME/ CFS met the criteria of Fukuda et al [9] and the Canadian criteria [10] for this condition [9, 10].
Screening questionnaires were used to evaluate the general health of the unaffected controls and to con rm that they did not meet ME/CFS case definition criteria. The ME/CFS and control sample sizes provided >95% power, with an α value <.05, to detect the same increase in CRP response as in the patients with PTLDS [5]. Group differences were assessed by the analysis of covariance, using the general linear model, to account for the potential confounding effect of age, sex, and BMI. is study was approved by the Institutional Review Board of Columbia University.
In contrast to data from patients with PTLDS [5], we did not fi nd a statistically significant difference in the circulating levels of CRP (Figure 1) or the frequency of CRP levels >3 mg/L (33 of 131 [25.2%] vs 22 of 86 [25.6%], respectively) between patients with ME/CFS and controls.
These data provide evidence for the likely existence of distinct in inflammatory mechanisms in ME/CFS versus PTLDS, which may be driven in part by the potentially more heterogeneous etiology of ME/CFS symptoms in comparison with PTLDS. e absence of a significantly enhanced CRP response in ME/CFS, despite published data suggesting activation of various in ammatory pathways, war- rants further examination.
(Figure 1. C-reactive protein (CRP) concentrations in the cohorts of patients with myalgic encephalomy- elitis/chronic fatigue syndrome (ME/CFS) and healthy controls (HCs). The difference between the 2 groups was not statistically signi cant (P = .55). Horizontal red bars represent the mean for each group.)
Melanie Uhde,1 Alyssa Indart,1 Brian A. Fallon,2 Gary P. Wormser,3 Adriana R. Marques,4 Suzanne D. Vernon,5 and Armin Alaedini1
Departments of 1Medicine and 2Psychiatry, Columbia University Medical Center, New York, and 3Division of Infectious Diseases, Department of Medicine, New York Medical College, Valhalla; 4Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; and 5Bateman Horne Center, Salt Lake City, Utah
To the Editor—There is substantial overlap in symptoms, including fatigue, muscle and joint pain, and cognitive and memory deficits, between post-treatment Lyme disease syndrome (PTLDS) and myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) [1]. Increasing evidence suggests a role for immunologic and inflammatory pathways in both PTLDS and ME/CFS [2–4]. However, in part owing to their etiologic complexity and the lack of established biomarkers, our understanding of the pathways involved and potential mechanistic differences between the 2 conditions is very limited.
In a 2016 study published in Clinical Infectious Diseases, Uhde et al [5] examined the concentrations of acute-phase response proteins, including C-reactive protein (CRP), in individuals with PTLDS. CRP is a highly sensitive marker of infection and inflammation that binds a variety of ligands present on the surface of pathogens or exposed during autologous cell stress or death, exerting its effect through opsonin deposition and activation of the complement path- way, in addition to direct interaction with phagocytic cells [6]. We found that the circulating levels of CRP, as well as the frequency of concentrations >3 mg/ mL (generally considered to represent some degree of inflammation [7]) to be significantly higher in the PTLDS cohort than in a control group of subjects who had a history of Lyme disease but with- out persistent symptoms (both P < .001). The data provided evidence for increased expression of an objective marker of inflammation in PTLDS but suggested a mechanism of activation distinct from that in active infection, as previously dis- cussed [5].
Using the same methods [5] in a new study, we screened plasma samples from 131 patients with ME/CFS (89 female; mean age [standard deviation], 50.0 [11.4] years; mean body mass index (BMI), 26.0 [5.5]) and 86 healthy controls (68 female; mean age, 50.0 [12.8] years; mean BMI, 26.5 [6.8]), provided by the SolveCFS BioBank [8]. Patients with ME/ CFS met the criteria of Fukuda et al [9] and the Canadian criteria [10] for this condition [9, 10].
Screening questionnaires were used to evaluate the general health of the unaffected controls and to con rm that they did not meet ME/CFS case definition criteria. The ME/CFS and control sample sizes provided >95% power, with an α value <.05, to detect the same increase in CRP response as in the patients with PTLDS [5]. Group differences were assessed by the analysis of covariance, using the general linear model, to account for the potential confounding effect of age, sex, and BMI. is study was approved by the Institutional Review Board of Columbia University.
In contrast to data from patients with PTLDS [5], we did not fi nd a statistically significant difference in the circulating levels of CRP (Figure 1) or the frequency of CRP levels >3 mg/L (33 of 131 [25.2%] vs 22 of 86 [25.6%], respectively) between patients with ME/CFS and controls.
These data provide evidence for the likely existence of distinct in inflammatory mechanisms in ME/CFS versus PTLDS, which may be driven in part by the potentially more heterogeneous etiology of ME/CFS symptoms in comparison with PTLDS. e absence of a significantly enhanced CRP response in ME/CFS, despite published data suggesting activation of various in ammatory pathways, war- rants further examination.
(Figure 1. C-reactive protein (CRP) concentrations in the cohorts of patients with myalgic encephalomy- elitis/chronic fatigue syndrome (ME/CFS) and healthy controls (HCs). The difference between the 2 groups was not statistically signi cant (P = .55). Horizontal red bars represent the mean for each group.)
