No clue on pH haha I don’t get that detailed usually unless I need to. Using distilled water 1-2ml and 1ml of lidocaine @ 20mg/ml.
I did 300mg nebulized last night and didn’t notice anything really in comparison so I may be sticking to the injectible route unfortunately
I haven't looked into SAM-e but there could be a chance that you are having issues with pH, especially since you didn't use a buffer. pH strips are cheap and can be useful. For example, when I first started making glutathione solutions I was using a buffer but didn't bother checking the pH. The solution very quickly goes to around pH 3 and it would be quite painful to inject (it would ease off quite quickly though as it would rebalance in the body). Adjusting this to around 6 with NaOH while mixing lets me get a much higher concentration in solution (200 mg/mL instead of 120-130 mg/mL), and it hurts a lot less.
If you take a look
here, you'll notice that the ampoule for injection has some sodium hydroxide in it. It is possible that when mixing your SAM-e it goes acidic, however, I'd be interested in what exact source you have. I'm currently waiting on a package with s-adenosyl-l-methionine disulfate p-toluensulfone, while this PDF discusses a 1,4-butanedisulfonate form. I'm not sure why they also include the l-lysine in this product, but perhaps there is some additional benefit (or it is just there as a lysine source? perhaps to ensure MTOR activation?).
Another issue to consider is your total concentration is too high and you are injecting a hyperosmolar solution. You would have to look into the dissolution of the SAM-e and the rest of the salt, whatever compound it is, and calculate the osmolarity. I doubt that it is above 900 though, which is where the real discomfort begins (eg high concentration magnesium solutions for injection are limited by this).
always something
