Cortisol not being consistent in blood and saliva

[roxie60]

hope this link works. This mentions another snp - rs12589136 I am GT. Dont know what the risl allele is

http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004474

 

[roxie60]

According to SNPedia AA on the negative? stand is the risk allele (TT?), dont take my mushy brain word for it DYODD

http://www.snpedia.com/index.php/Rs28929488

Rs28929488 (for those with Firefox SNP plugin - what a great plugin)

I am CC on this snp so guess not the cause/contributor to my cortisol issues (if I have any).

 

[roxie60]

Im losing track so sorry if I posted this already but I think this very interesting now just need to line up GENE with rs#s/snps

http://press.endocrine.org/doi/pdf/10.1210/jc.2011-3141

This is what I focused on in the conclusions

CBG A51V bound steroid normally, but
its production/secretion was severely impaired; CBG E102G was produced normally, but its cortisolbinding
capacity was abnormally low, whereas CBG R64Q and R64W were produced and bound
cortisol normally.

So if I have not inadvertently already listed the SNPs for these genes Id be curious if we can figure the rs#s out.

 

[roxie60]

This study found 8 snps to be significantly associated with CBG. As typical 23andme only provides info on 2, they probably have them but not listed as rs#...argh

http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092957

For those who have more energy than I do right now here is a fun list. Hope it pastes ok

HPA outcome
Gene     SNP      Beta       P-value
Calculated Plasma Free Cortisola CRHR1 rs17763104 20.105 0.023
NR3C1 rs17287745 20.098 0.035
NR3C1 rs10482642 0.083 0.057
CRHR1 rs171440 0.062 0.049
Plasma Total Cortisol SERPINA6 rs11621961 20.041 0.059
NR3C2 rs11099694 0.040 0.056
NR3C2 rs13148853 0.053 0.021
MC2R rs8093607 0.044 0.038
CRHR1 rs17763104 20.070 0.021
NR3C2 rs2883930 0.060 0.009
NR3C1 rs10482642 0.061 0.030
Plasma CBG SERPINA6 rs11622665 20.056 0.005
SERPINA6 rs7161521 0.044 0.012
SERPINA6 rs941601 0.061 0.004
NR3C2 rs13105361 0.046 0.012
SERPINA6 rs1956178 20.053 0.004
NR3C2 rs11724292 20.047 0.016
NR3C2 rs4635799 0.036 0.018
OXT rs2770378 20.033 0.023
Plasma ACTH NR3C2 rs907621 20.094 0.002
NR3C2 rs17024708 20.057 0.027
NR3C2 rs5522 20.101 0.005
NR3C2 rs3846326 20.078 0.003
NR3C2 rs17484063 20.067 0.015
NR3C2 rs13148853 0.073 0.003
CRHR2 rs2267710 20.066 0.005
NR3C2 rs17484601 20.054 0.017
NR3C2 rs13116099 0.055 0.014
CRHR2 rs2190242 20.066 0.014
CRHR2 rs2267716 20.056 0.025
Saliva Cortisolb NR3C2 rs879206 0.092 0.010
NR3C2 rs1994624 0.068 0.016
OXT rs2770378 0.053 0.053
NR3C2 rs3910044 0.057 0.038

 

[roxie60]

Why cant all studies post the gene name and rs#s like the study above!!! Now just have to fgure out the risk alleles. Ok I looked further and this study did much rs# work for us. If Im interpreting correctly looks like TT is the risk allele, please correct me if you find not to be true

Outcome variable rs11621961
genotype N Median (IQR) Betaa P-value
Total plasma cortisol (nmol/L) CC 340 606.91 (498.10, 715.70) 20.041 0.059
CT 370 574.24 (467.50, 678.50)
TT 92 558.70 (450.80, 696.50)
Plasma CBG (mg/ml) CC 336 48.22 (40.87, 60.41) 20.028 0.079
CT 369 45.98 (40.05, 53.62)
TT 91 44.88 (39.47, 55.41)

 

[roxie60]

Just putting these here as ref only to Gene and rs#s

Calculated Plasma Free
Cortisolb
CRHR1 rs4458044 GG GC CC
SERPINA6 rs11160169 CC CA AA
Plasma CBG NR3C1 rs2963156 CC CT/TT
Plasma ACTH AVP rs3761249 TT TG/GG
CRHR1 rs242942 GG GA/AA
Saliva Cortisolc CRHR2 rs1076292 CC CG GG
NR3C2 rs17484601 TT TG GG

 

[roxie60]

Did anyone else with adrenal issues look at these?

 

[ukxmrv]

snps from cbg articles, not sure they are correct

Two SNPs in SERPINA6 (rs941601 and rs8022616)

I am rs941601 CC and rs8022616 AA I do not know the risk allele

I'm also CC and AA

 

[Valentijn]

I'm also CC and AA

70-74% of Europeans are CC for rs941601, and 82% of Europeans are AA for rs8022616.

They probably don't have any impact.

 

[drob31]

I think this is saying the 2 snps in the cbg gene that cause problems are rare

http://www.ncbi.nlm.nih.gov/pubmed/22337907

CBG A51V bound steroid normally, but its production/secretion was severely impaired; CBG E102G was produced normally, but its cortisol-binding capacity was abnormally low, whereas CBG R64Q and R64W were produced and bound cortisol normally.

It's rs146744332

Other SERPINA6 variants have been identified in Chinese populations [38], where CBG A51V (rs146744332) heterozygotes were found to have 50% lower CBG levels than non-carriers

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0092957

 

[drob31]

By the way I'm ++ for rs11621961

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0092957

SERPINA6 rs11621961

This may be the adrenal fatigue gene.

 

[lisette.w]

[QUOTE

Why when the tests are performed by allopaths my basal cortisol is so high? Is blood a better measure as opposed to what we are told in the Internet or it is not? [/QUOTE]


My daughter had the same problems...the answer was ..drop the current birth control (chemical pill) changed to a natural pill.
Your current pill gives a false positive.
And test again your morning cortisol..it will be much lower.

 

[Valentijn]

By the way I'm ++ for rs11621961

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0092957

The SNP probably isn't significant. They had to raise the p-value to 0.06 to get it included (normal limit would 0.05 or lower). And even if it were significant, the calculated impact is quite small - it would not cause cortisol problems by itself.

 

[drob31]

The SNP probably isn't significant. They had to raise the p-value to 0.06 to get it included (normal limit would 0.05 or lower). And even if it were significant, the calculated impact is quite small - it would not cause cortisol problems by itself.

But what if you have multiple hetero/homozygous SNP's? I have 2 on SERPINA6, and 1 on this one. The way I understand it is that hypothetically you wouldn't produce enough CBG, but you would never notice this unless your HPA-axis became dysregulated, and then at some point the high levels of cortisol would cause the cortisol receptors and thyroid receptors on the cells to resist hormones when cortisol was too high.

 

[Valentijn]

But what if you have multiple hetero/homozygous SNP's?

If they haven't been shown to cause a statistically significant impact, it can't be assumed that having multiple rarer SNPs (if they're actually rare at all) is relevant.

The way I understand it is that hypothetically you wouldn't produce enough CBG, but you would never notice this unless your HPA-axis became dysregulated, and then at some point the high levels of cortisol would cause the cortisol receptors and thyroid receptors on the cells to resist hormones when cortisol was too high.

It's too much guess-work to be able to draw any conclusions from it, in my opinion.