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xmrv needs 4 tests (Nov 09)

Discussion in 'XMRV Testing, Treatment and Transmission' started by leelaplay, Nov 3, 2009.

  1. leelaplay

    leelaplay member

    Gracenote highlighted the following excerpt from Dr Bell's newsletter that imready posted. http://www.davidsbell.com/PrintLynNewsV6N3.htm

    I thought it was important enough to have it's own thread.

  2. _Kim_

    _Kim_ Guest

    Note that the tests that Dr. Bell speaks of are (at the moment) the different ways that XMRV can be detected. So, if your tests from VIPdx (PCR and antibody) are negative, you still may test positive by one of the other tests.

    From the VIPdx website:
    However, if your PCR and/or antibody test are positive, there's probably no need to have the others done.
  3. Aftermath

    Aftermath Guest

  4. mojoey

    mojoey Senior Member


    Dr. Peterson told me if I have a neg. PCR, there's no reason to get the other tests done because it's not active. The PCR is the screener.
  5. kamina

    kamina Retired account

    Also note that Dr.Bell says the following at the end of the newsletter about testing:

  6. Aftermath

    Aftermath Guest

    Link to Testing Thread

    Here is a link to islandfinn's "4 test thread."

    FYI, you guys have the ability to do links. Just highlight the globe with the paperclip icon above the posting window and paste in the URL of the thread you wish to link to.
  7. citybug

    citybug Senior Member


    But if you are positive then you want them for....? To measure progess?
  8. myco

    myco Guest

    I agree with Bell. What I have been saying all along.
  9. nora_n


    Lyme patients know that lyme losers the immune response very much, so that very sick lyme patients often ahve entirely negative tests. That is ELISA tests for lyme antibodies 8=immune syetem's response to the pathogen) and western blots (that is wetsern blots for antibodies to different protein parts of lyme bacteriae, and lyme really hidess it surface proteins, and lowers the immune system, like cd-57)

    Now xmrv attacks the b cells adn lowers immunity, is there a problem like with lyme, that sick patients test negative becasue they are so sick?
    Of course , PCR tests against the actual pathogen and is not antibody dependent.
    Now with lyme, they usually have to take that test several times before a lab finds the needle in the heystack. That is because lyme is not so much in teh blood, but in cartilage and the cns, where body immune cells do not circulate so much, neither do antibiotics...so it is not quite the same.

    But antibody testing, that is dependent on antibodies; western blot, is that to antibodies too?
  10. CBS

    CBS Senior Member

    Controls and more thorough testing?

    Does anyone know if any of the controls were subjected to the more thorough analysis? I wonder what those numbers would look like.

    Lots of questions all around!

  11. mojoey

    mojoey Senior Member


    I didn't have time to ask him, but I guess to determine severity and progress.

  12. parvofighter

    parvofighter Senior Member

    Negative bloodwork - a showstopper?

    Hi @ Lady, you raised an excellent point about whether one can have virus in tissues, but not in blood:

    From what I can discern of the available research, we just don't know that yet for XMRV. BUT we sure as heck do for some of the opportunistic viruses. Dr Ablashi, Scientific Director of the HHV-6 Foundation writes an excellent precis on the dangers of assuming that bloodwork is THE answer. He cites several specific instances of where tissue investigations are required (eg. PCR, immunohistochemistry) in lieu of bloodwork, in his letter to CFSAC: http://www.hhs.gov/advcomcfs/meetings/presentations/ablashi_1009.pdf

    One of his key points:
    "The CDC has made several assumptions that we believe will prove to be in error:
    1. The CDC has assumed that if a pathogen exists, it can be found in the serum"

    "It is imperative that the CDC study biopsy samples from the gut, and brain as well as heart tissues, and
    that they look at spinal fluid. Most of the studies done by the CDC have been on serum. However, many
    pathogens cannot be found in the serum because they do not circulate in the peripheral blood after the
    initial infection."

    This diagnostic oversight is absolutely vital for us patients to understand, because we're talking about chronic, years/decades long infections. Namely, when is bloodwork adequate, and when does it yield false negative results? Despite all the vampire hype in the media, blood isn't always all it's cracked up to be. Because false negative in bloodwork = no treatment.

    Here are a few tidbits from my digging through the chronic Parvovirus B19 research, another known virus associated with ME, which is known to present diagnostic challenges with bloodwork:

    Over-reliance on unreliable bloodwork: The Dangers
    • From Harvard: In some low viremic states, parvovirus B19 DNA is detectable by nested PCR in plasma but not in serum. S. Kim Jacobson, Jennifer S. Daly, Grace M. Thorne, and Kenneth McIntosh. Chronic Parvovirus B19 Infection Resulting in Chronic Fatigue Syndrome: Case History and Review (From the Division of Infectious Diseases, Department of Pediatrics, Harvard Medical School). Clinical Infectious Diseases 1997; 24:104851 : http://www.journals.uchicago.edu/doi/pdf/10.1086/513627
    • The diagnosis (of PVB19) is based on serology, bone marrow examination to demonstrate pure red cell aplasia, and PCR tests to demonstrate the presence of parvovirus B19 DNA in clinical specimens. Use of serology is limited due to failure to mount immune response in some patients. Schlossberg, David. Clinical Infectious Diseases. Part XI: The Susceptible Host, pp. 620-621. Cambridge University Press, May 2008. http://books.google.ca/books?id=-wW...m=23#v=onepage&q=acyclovir parvovirus&f=false
    • immunocompetent individuals who have evidence of current or recent infection may lack detectable levels of B19 DNA in their blood when tested by PCR. Evidence to support this came from a study of 40 immunocompetent individuals, who were tested for B19 DNA by PCR. All had shown evidence of seroconversion, B19 IgM and B19 IgG positive. Of these 40, only 45%(18/40) contained B19 DNA using non-nested PCR, whereas the remaining 55% of samples were negative by PCR for B19V DNA. It is suggested that based on this data, PCR should not be used to detect B19V acute infection in immunocompetent persons. Diagnosing human Parvovirus B19 infection: Guidelines for test selection. Jordan J. Molecular Diagnosis 2001:6:307-312. See page 3 of URL: http://www.fifthdisease.org/cmsFiles/fifthdisease_human_literature_reveiw.pdf
    • It is well known that the B19 genome can be detected in tissue without concurrent viremia as well as that negative serological test results during acute B19 infection can be seen in the first months after HSCT (hematopoietic stem cell transplantation) and It has to be noted that not all assays are capable of detecting the B19 genotypes 2 and 3. F Beske, S Modrow, J Srensen, H Schmidt, S Kriener, R Allwinn, T Klingebiel, D Schwabe and T Lehrnbecher , Parvovirus B19 pneumonia in a child undergoing allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplantation (2007) 40, 8991; doi:10.1038/sj.bmt.1705693; published online 30 April 2007. http://www.nature.com/bmt/journal/v40/n1/full/1705693a.html

    Here's an example of how this plays out for patients with ME and chronic PVB19. The reality emerges that legions of chronic PVB19 patients are denied proper diagnosis and further investigation as a result of flawed emphasis on bloodwork which may itself be flawed! To whit: my PCR for PVB19 was negative in Canada, but my clinical presentation was exactly like that of Kerr's PVB19 chronic fatigue patients. So while it may be well known in German cardiology circles that B19 genome can be detected in tissue without concurrent viremia, this is certainly not my experience in Canada, where it took 10 years of bull-headed insistence and a trip to Germany for a biopsy to prove that I had chronic PVB19.
  13. parvofighter

    parvofighter Senior Member

    Active/reactivated vs latent virus

    Oops - forgot to add... the other consideration is that if you get tested for any virus, you ideally want to know:
    - Do I have the virus (the <4% of controls reported by Mikovits)
    - Is it active/reactivated, or latent?

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