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Abstract
Background:
Studies examining herpesvirus-herpesvirus (cytomegalovirus (CMV)-Epstein-Barr
virus (EBV)) interactions are limited, and many of the studies have been clinical observations
suggesting such an interaction exists. This report aims to examine the
in vitro susceptibilities of
BJAB-B1 and P3HR-1 cells (EBV positive Burkitt's lymphoma B-cell lines) to a CMV superinfection;
and show that EBV reactivation occurs after CMV superinfects these cell lines.
Results:
The BJAB-B1 and P3HR-1 cells were observed to be susceptible to a CMV superinfection
by the detection of the major immediate early (MIE) viral transcript and protein (p52) expression.
The BZLF1 transcript was observed in both cell lines superinfected with CMV, indicating EBV
reactivation. BZLF1 protein was observed in the BJAB-B1 cells. Antigen detection was not
performed in the P3HR-1 cells.
Conclusion:
The results from the in vitro superinfections support the in vivo studies suggesting a
CMV infection is related to an EBV reactivation and suggests that CMV may be important as a cofactor
in EBV pathogenesis in the immunocompromised patient.
http://www.biomedcentral.com/content/pdf/1471-2180-2-20.pdf
Background:
Studies examining herpesvirus-herpesvirus (cytomegalovirus (CMV)-Epstein-Barr
virus (EBV)) interactions are limited, and many of the studies have been clinical observations
suggesting such an interaction exists. This report aims to examine the
in vitro susceptibilities of
BJAB-B1 and P3HR-1 cells (EBV positive Burkitt's lymphoma B-cell lines) to a CMV superinfection;
and show that EBV reactivation occurs after CMV superinfects these cell lines.
Results:
The BJAB-B1 and P3HR-1 cells were observed to be susceptible to a CMV superinfection
by the detection of the major immediate early (MIE) viral transcript and protein (p52) expression.
The BZLF1 transcript was observed in both cell lines superinfected with CMV, indicating EBV
reactivation. BZLF1 protein was observed in the BJAB-B1 cells. Antigen detection was not
performed in the P3HR-1 cells.
Conclusion:
The results from the in vitro superinfections support the in vivo studies suggesting a
CMV infection is related to an EBV reactivation and suggests that CMV may be important as a cofactor
in EBV pathogenesis in the immunocompromised patient.
http://www.biomedcentral.com/content/pdf/1471-2180-2-20.pdf