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http://admbio.ccu.edu.tw/new/seminar_pdf/991/1022B2.pdf
Enhanced susceptibility of T lymphocytes to oxidative stress
in the absence of the cellular prion protein
Catherine Aude-Garcia • Christian Villiers • Serge M. Cande´ias •
Catherine Garrel • Caroline Bertrand • Ve´ronique Collin • Patrice N. Marche •
Evelyne Jouvin-Marche
Accepted: 21 July 2010 Springer Basel AG 2010
Abstract
The cellular prion glycoprotein (PrPC) is
ubiquitously expressed but its physiologic functions remain
enigmatic, particularly in the immune system. Here, we
demonstrate in vitro and in vivo that PrPC is involved in T
lymphocytes response to oxidative stress. By monitoring
the intracellular level of reduced glutathione, we show that
PrP-/- thymocytes display a higher susceptibility to H2O2
exposure than PrP?/? cells. Furthermore, we find that in
mice fed with a restricted diet, a regimen known to increase
the intracellular level of ROS, PrP-/- thymocytes are more
sensitive to oxidative stress. PrPC function appears to be
specific for oxidative stress, since no significant differences
are observed between PrP-/- and PrP?/? mice exposed to
other kinds of stress. We also show a marked evolution of
the redox status of T cells throughout differentiation in the
thymus. Taken together, our results clearly ascribe to PrPC
a protective function in thymocytes against oxidative
stress.
Enhanced susceptibility of T lymphocytes to oxidative stress
in the absence of the cellular prion protein
Catherine Aude-Garcia • Christian Villiers • Serge M. Cande´ias •
Catherine Garrel • Caroline Bertrand • Ve´ronique Collin • Patrice N. Marche •
Evelyne Jouvin-Marche
Accepted: 21 July 2010 Springer Basel AG 2010
Abstract
The cellular prion glycoprotein (PrPC) is
ubiquitously expressed but its physiologic functions remain
enigmatic, particularly in the immune system. Here, we
demonstrate in vitro and in vivo that PrPC is involved in T
lymphocytes response to oxidative stress. By monitoring
the intracellular level of reduced glutathione, we show that
PrP-/- thymocytes display a higher susceptibility to H2O2
exposure than PrP?/? cells. Furthermore, we find that in
mice fed with a restricted diet, a regimen known to increase
the intracellular level of ROS, PrP-/- thymocytes are more
sensitive to oxidative stress. PrPC function appears to be
specific for oxidative stress, since no significant differences
are observed between PrP-/- and PrP?/? mice exposed to
other kinds of stress. We also show a marked evolution of
the redox status of T cells throughout differentiation in the
thymus. Taken together, our results clearly ascribe to PrPC
a protective function in thymocytes against oxidative
stress.