Bioinformatics collaboration

[undiagnosed]

Hey,

I want to revive a project I started working on to do nucleic acid amplification primarily for the purposes of pathogen detection and discovery with minimal equipment requirements using a Loop-Mediated Isothermal Amplification (LAMP) based assay. I am looking for anyone with bioinformatics experience to help by providing peer review and advice. My interest is in lentiviruses, but we could do other pathogens as well. I am currently working on designing primers, but it's my first time so I am trying to figure out sequence alignment and using tools like re-pcr and e-lamp to determine primer specificity in-silico prior to getting the primers manufactured.

Here is a thread I started a while ago which has some initial research I did. It was more focused on detecting reverse transcriptase activity but still using a LAMP assay.

I also have a few documents started including, RT-LAMP assay for detecting lentiviruses and A minimal equipment one-pot RT-LAMP assay for detecting reverse transcriptase activity.

Let me know if you are interested in helping out or know anyone who may be.

Thank you