International ME/CFS and FM Awareness Day Is On May 12, 2018
Thomas Hennessy, Jr., selected May 12th to be our international awareness day back in 1992. He knew that May 12th had also been the birthday of Florence Nightingale. She was the English army nurse who helped to found the Red Cross as well as the first school of nursing in the world.
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Bioinformatics collaboration

Discussion in 'Other Health News and Research' started by undiagnosed, Jun 16, 2017.

  1. undiagnosed

    undiagnosed Senior Member

    United States

    I want to revive a project I started working on to do nucleic acid amplification primarily for the purposes of pathogen detection and discovery with minimal equipment requirements using a Loop-Mediated Isothermal Amplification (LAMP) based assay. I am looking for anyone with bioinformatics experience to help by providing peer review and advice. My interest is in lentiviruses, but we could do other pathogens as well. I am currently working on designing primers, but it's my first time so I am trying to figure out sequence alignment and using tools like re-pcr and e-lamp to determine primer specificity in-silico prior to getting the primers manufactured.

    Here is a thread I started a while ago which has some initial research I did. It was more focused on detecting reverse transcriptase activity but still using a LAMP assay.

    I also have a few documents started including, RT-LAMP assay for detecting lentiviruses and A minimal equipment one-pot RT-LAMP assay for detecting reverse transcriptase activity.

    Let me know if you are interested in helping out or know anyone who may be.

    Thank you
    Last edited: Jun 17, 2017

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