The in vivo infusion of hydrogen peroxide induces oxidative stress and differentially affects the activities of small intestinal carbohydrate digestive enzymes in the neonatal pig1
D. Lackeyram*,
Y. Mine†,
T. Widowski*,
T. Archbold* and
M. Z. Fan*,2
J Anim Sci. 2012 Dec;90 Suppl 4:418-20. doi: 10.2527/jas.54011.
http://www.ncbi.nlm.nih.gov/pubmed/23365398
http://www.journalofanimalscience.org/content/90/Supplement_4/418.abstract
[I added paragraph breaks]
[the jejunum is a specific region in the intestine]
D. Lackeyram*,
Y. Mine†,
T. Widowski*,
T. Archbold* and
M. Z. Fan*,2
J Anim Sci. 2012 Dec;90 Suppl 4:418-20. doi: 10.2527/jas.54011.
http://www.ncbi.nlm.nih.gov/pubmed/23365398
http://www.journalofanimalscience.org/content/90/Supplement_4/418.abstract
Chronic fatigue syndrome (CFS) is characterized by persistent and relapsing fatigue that involves oxidative stress in its pathogenesis.
We tested the hypothesis that a decrease in key carbohydrate-digesting enzyme activity in the gut is one of the major biological mechanisms of developing CFS in liquid formula-fed neonatal pigs with in vivo infusion of H2O2.
Piglets at 7 to 10 d of age were fitted with an intraperitoneal catheter, allowed a 3-d post surgical recovery, and infused with either H2O2 at 5 mmol/kg BW (PER; n = 8) or the same volume of saline (CON; n = 8) in six 20-ml doses daily for a period of 10 d.
During this period, animal behavior was monitored, blood samples collected, and jejunal enzyme activity kinetic experiments for lactase, sucrase, maltase, and maltase-glucoamylase were conducted.
Plasma concentration of reduced glutathione remained similar (P > 0.05) to the pre-infusion level over the study duration in the CON group whereas this was 65% lower (P < 0.05) than the pre-infusion level in the PER group.
Piglets experiencing oxidative stress had an overall lower (P < 0.05) physical mobility and the maximal jejunal specific activities [μmol/(mg protein·min)] for lactase (PER, 6.54 ± 0.68 vs. CON, 12.65 ± 0.69) and maltase (PER, 57.39 ± 1.02 vs. CON, 75.60 ± 1.04), respectively.
However, differences were not observed (P > 0.05) in the maximal specific activities [μmol/(mg protein·min)] of sucrase (PER, 10.50 ± 1.37 vs. CON, 12.40 ± 1.55) and maltase-glucoamylase (PER, 0.71 ± 0.08 vs. CON, 0.70 ± 0.07) between the 2 groups.
In conclusion, infusion of a suitable dose of H2O2 induced CFS in the neonatal pigs.
Oxidative stress in vivo differentially affected the maximal activities of important small intestinal carbohydrate-digesting enzymes in neonatal pigs fed a dairy milk-based liquid formula.
[I added paragraph breaks]
[the jejunum is a specific region in the intestine]