Bead Dog,
To clarify, are you talking about Autism: Pathways to Recovery by Dr. Amy Yasko? If so, then chapter 6 would be a good read for many, in explaining the pathways relevant to your mutations, and some additional info on what their function is. You can also do internet searches cross referencing Dr. Amy Yasko, with XXXX (for example SHMT) and you will get specific information as well. I think many here, including myself start supplementing the methylation pathway specifically with the methylfolate and active b's too soon and experience awful symptomology, sometimes as disabling as CFS/ME itself.
After doing it the wrong way, and becoming more familiar, I completely agree with her recommendations concerning her first priority mutational targets. CBS mutations are one of them. Specifically the third of three first priority mutations. Although the 360A mutation (CBS) is not considered as significant a mutation as the C699T (40 something increase in upregulation), it is still an upregulation. And this upregulation is what contributes to the swing of supportive intermediates to be drained down this pathway and shunted to either Taurine or depending on further mutations, excess sulfite (SOUX downregulation), or excess sulfate (SOUX upregulation). I see nothing in your post about SOUX. The strips would be helpful in determining whether you have a SOUX problem by identifying whether you have excess sulfite or excess sulfate, but remember, deficiencies can mimic other conditions even though a mutation may not exist. So the strips would not offer a definitive evaluation. Also in this cycle, you would have to consider ACHY mutations as well. I also see that mutation not listed in your post, but it would have an impact on Hcy.
Another impact on Hcy (homocystiene), is your BHMT mutations. This is the shortcut through the transsulfuration pathways to convert homocystiene to methionine. These are down regulations, and would affect your levels of SAMe, and also affect your ability to methylate DNA and RNA.
So the long way around the beginning of the cycle has MTRR mutation, and then you have the CBS mutation, and the BHMT mutation which has downregulation the short cut through the cycle as well. The long way and the short way are downregulated and the CBS is upregulated.
In this context, you will also need to consider ammonia, and its relation to sulfate, hence the SOUX again. Diet, especially protein can produce sulfur metabolites, of which your CBS mutation contributes and excaberates. Diet didn't "fix" my problem, although it did help.
Another consideration when evaluating your sulfur problem, is chronic bacterial and viral infections. These steal tryptophan, and lead to sulfur and ammonia problems as well as heavy metal accumulations. The kind of bacterial or viral infections, will affect which metals are correlated as problematic. Example, most chronic bacterial infections will sequester aluminum. So this then can affect your MAOA status, and serotonin levels, and could manifest as the wired but tired, buzzy neuro problems, and sleep cycle problems should you have this as well. Addtionally, BHMT 08 +/+ can also lead to nor-epi, also contributing to a wired but tired, buzzy neuro issue. I don't have the BHMT mutations, but I did have a big problem with nor-epi. Dr. Yasko states high levels of stress and cortisol production as inducers of nor-epi as well.
To support your BHMT shortcut, TMG supports it, DMG shuts it down and shifts the metabolites through the long way of the cycle, but your long way has an MTRR mutation. Molybdenum is also recommended for both (CBS and sulfur metabolism, ammonia, and the BHMT)
Molybdenum as a caution, was a biggie for me. Makes you downright fluey for about a week, and then it seems to clear. I have done experiments with moly three times, and each time I experience the same thing, no matter how far along I have come.
So by addressing CBS and then the BHMT and MTRR, you will level out your SAMe, Methionine, Hcy, sulfur, and ammonia. Then the long way around the folate cycle in which the forward reaction, MTHFR 677 is also of concern to you and another possible roadblock.
All this is just going off the possibilities of mutational aspects, and not off of specific metabolites showing in your urine, by testing. That is what I lack, and so recommendations made by anybody will be refined to your own symptoms. Those of which I am unfamiliar and I am certainly not an expert, just trying to help.
The supportives I will address in another post.
I did want to provide a link for those interested for the Autism: Pathways to Recovery book, should one not possess the book. The entire book is available at this link.
http://www.scribd.com/doc/81963801/113/BHMT-Status
I apologize I wasn't able to respond earlier, I do work two jobs, and it is why I must reply in two parts.
Lauriel