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Green tea, anyone?
Immunopharmacol Immunotoxicol. 2011 Jun;33(2):391-7. doi: 10.3109/08923973.2010.522195. Epub 2010 Oct 11.
Catechin inhibits adhesion and migration of peripheral blood B cells by blocking CD11b.
Kawai K, Tsuno NH, Kitayama J, Sunami E, Takahashi K, Nagawa H.
Source
Department of Surgical Oncology, Faculty of Medicine, University of Tokyo, Tokyo, Japan. kz-kawai@mvd.biglobe.ne.jp
Abstract
Context: Previously, we demonstrated that CD11b is expressed on peripheral blood memory B cells, and it plays an important role in the migration of B cells. And epigallocatechin gallate (EGCG), a bioactive component of green tea, by binding to CD11b, expressed on CD8(+) cytotoxic T cells, inhibited their migratory ability, one possible mechanism of the antiallergic activity of EGCG. Objective: Here, we investigated whether EGCG also affected CD11b expressed on B cells, similar to cytotoxic T cells. Materials and methods: Isolated peripheral blood CD19(+) B cells were treated with EGCG and the change in the expression of CD11b was analyzed using flow cytometry. The effects of EGCG on the ability of B cells to adhere to and to transmigrate through the endothelial cell layer were evaluated using the transwell assay. Results: EGCG significantly suppressed the apparent expression of CD11b on B cells, in the flow-cytometric analysis, and this apparent suppression was speculated to be dependent on the competitive binding of EGCG to CD11b. EGCG also significantly suppressed CD11b-mediated migration and adhesion of B cells to endothelial cells. Discussion and conclusion: EGCG has a strong suppressive activity on the adhesive and migratory abilities of peripheral blood B cells. This suppressive activity was mediated by the binding of EGCG to CD11b on B cells, and the consequent suppression of B-cell extravasation to the extravascular space. Because B cell plays an important role in the humoral immunity, EGCG could be a promising drug for the prevention and/or treatment of allergic and/or autoimmune diseases.
PMID:
20936888