- Messages
- 43
First, these are my transulfuration related SNPS:
My main symptoms: anxiety, heart palpitations, GERD, rhinitis with no mucus, higher blood pressure, internal "shakiness", intention tremor.
I have been doing very well following a low protein, low non-essential amino acid, low thiol diet and taking varying amounts of pyridoxine 5 phosphate (B6 - 25mg to 100mg subligual) among other supplements, but there would always seem to be times when the B6 made me feel worse or that a food that was low in thiols would trigger slight sulfite reaction. What I stumbled upon this morning was serine.
Serine, a non-essential amino acid, is a cofactor for the CBS enzyme to make cysteine. I cannot eat eggs at all, and this makes sense because they are not only high in sulfur, they are high in serine.
So, some notes on serine and B6:
It makes me also think that B6 supplementation is not needed as much if you have low serine intake, and that supplementing with the B6 increases the transsulfuration pathway action and could make us feel worse.
(As an aside to ponder, I think the key to ME/CFS lies in the TCA cycle and how that cycle is driven my different macro nutrients. And I think that calorie restriction is important to to those of us with these mutations. Calorie restriction influences the SIRT3 gene which changes the TCA cycle.)
- CBS A13637G rs2851391 CT +/- Heterozygous
- CBS C19150T rs4920037 AG +/- Heterozygous
- CBS C699T rs234706 AA Homozygous
- SUOX rs10876864 AG Heterozygous
My main symptoms: anxiety, heart palpitations, GERD, rhinitis with no mucus, higher blood pressure, internal "shakiness", intention tremor.
I have been doing very well following a low protein, low non-essential amino acid, low thiol diet and taking varying amounts of pyridoxine 5 phosphate (B6 - 25mg to 100mg subligual) among other supplements, but there would always seem to be times when the B6 made me feel worse or that a food that was low in thiols would trigger slight sulfite reaction. What I stumbled upon this morning was serine.
Serine, a non-essential amino acid, is a cofactor for the CBS enzyme to make cysteine. I cannot eat eggs at all, and this makes sense because they are not only high in sulfur, they are high in serine.
So, some notes on serine and B6:
- The SHMT gene uses B6 to take up Serine to convert THF to 5,10 methylene THF. Here is another image.
- B6 is a cofactor to produce myelin via the serine palmitoyltransferase (SPT) enzyme.
- Serine is a driver of the TCA (krebs) cycle via Pyruvate, as is cysteine interestingly.
- This is also interesting: Cysteine metabolism in vivo of vitamin B6-deficient rats. Which shows that the L-cysteine catabolism in B6 deficient rats increases pyruvate but not taurine.
- During dietary protein restriction, it was found that de novo serine synthesis increased, but... Serine synthesis normally occurs at the kidney, but under these conditions it also takes place at the liver.
- The administration of ample amounts of pure B vitamins to rats on an experimental diet, receiving dl-serine by stomach tube, reduces considerably the mortality and the severity of the clinical symptoms. Of the B vitamins tested, pyridoxine was most effective.
It makes me also think that B6 supplementation is not needed as much if you have low serine intake, and that supplementing with the B6 increases the transsulfuration pathway action and could make us feel worse.
(As an aside to ponder, I think the key to ME/CFS lies in the TCA cycle and how that cycle is driven my different macro nutrients. And I think that calorie restriction is important to to those of us with these mutations. Calorie restriction influences the SIRT3 gene which changes the TCA cycle.)