This is not a evidence baced study. It is private.
My wife has been sick for over 7 years. She became ill in autumn 2008 by a tick bite. 2012 she was diagnosed with ME / CFS. She never received any treatment for Lyme disease. A disease that I suspected from the beginning. She got negative results by ELISA tests. A test that is completely useless on the basis of international studies available.
http://www.aonm.org/assets/dr.-armin-schwarzbach-aonm-lifting-the-veil.pdf
Autumn 2015 she made a test of a Chinese bachelor. With dark field microscopy we could clearly see that she had spirochetes in her blood. And even babesios, barton and mycoplasma.
I have read almost all studies of ME / CFS (+ 2000 hours) and understood that there are no biomarkers - yet. You can read my summary here - Please translate with Google Translate. I hope you will understand that I know a lot.
https://newsaboutdisease.wordpress.com/
When I was suspecting she might have Lyme disease, I contacted a biomedical scientist who worked in a laboratory for several years, and also served on the education of Bela Bozsik in Hungary. Her name is Titti Alvarsson.
Titti is using a special method to show whether you have Lyme disease or not. Frequently terapeuet using capillary blood. It's simple. You can just take a finger prick. Adding drop under a microscope. But it is far from enough. You have to take venous blood, and you have to centrifuge the blood!.
During centrifugation, the plasma separates from the blood cells. Experience shows that any spirochetes, if available, accumulate in the area between the plasma and blood cells. Precisely this is important to remember! Titti was mad at me because I revealed this method. But, I think it is important that everyone learns. The accumulation of "middle" is placed under a microscope (darkfield). Then we can see if there are spirochetes or not.
I am a director of a FB group of more than 1100 patients with ME / CFS in Sweden. I asked who was interested to be part of an investigation. I got together 40 patients. All paid individually for what it cost to carry out the investigation. A total of about 60 000 SEK. Approximately $ 8,000.
I have not received a penny my self. I have only done this to show that there may be a possibility that a subgroup of ME / CFS may actually be suffering from Lyme disease.
I do not claim that it is Lyme disease. But evidence suggests that, definitely when you see all the symptoms of patients who participated.
Thanks
Mats Lindstrom
Sweden