pattismith
Senior Member
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β1-adrenergic and Muscarinic Acetylcholine Type 2 Receptor Antibodies are Increased in Graves’ Hyperthyroidism and Decrease During Antithyroid Therapy
Abstract
Objective
To determine the association between autoantibodies to G-protein-coupled receptors with effect on the cardiovascular system and the cardiac biomarker N-terminal pro-brain natriuretic peptide reflecting heart function in Gravesʼ disease.
Design and Methods
Sixty premenopausal women with Graves’ disease were analyzed for IgG autoantibodies against
β1-adrenergic,
muscarinic acetylcholine type 2 and
angiotensin II type 1 receptors
using enzyme-linked immunosorbent assays based on cell membranes overexpressing receptors in their native conformations.
N-terminal pro-brain natriuretic peptide and heart symptoms were analyzed in hyperthyroidism and after 7.5 months of antithyroid treatment.
Matched thyroid healthy controls were also assessed.
Results
Serum levels of antibodies against the β1-adrenergic and the muscarinic acetylcholine type 2 receptors were higher in hyperthyroid patients than in controls
(median β1-adrenergic receptor antibodies 1.9 [IQR 1.3–2.7] vs. 1.1 [0.8–1.7] μg/mL, P<0.0001; muscarinic acetylcholine type 2 receptor 20.5 [14.0–38.3] vs. 6.0 [3.2–9.9] U/mL, P<0.0001).
These antibodies decreased in euthyroidism (P<0.01), but were still higher than in controls (P<0.01).
Angiotensin II type 1 receptor levels did not differ.
N-terminal pro-brain natriuretic peptide was higher in hyperthyroidism (240 [134–372] vs. <35 [<35–67] ng/L, P<0.0001), normalized after treatment and did not correlate with autoantibodies.
Conclusion
Autoantibodies against the β1-adrenergic and the muscarinic acetylcholine type 2 receptors were increased in Graves’ patients, decreased with treatment, but did not correlate with cardiac function.
However, an autoimmune effect on the heart cannot be excluded in subpopulations, as the functional properties of the analyzed antibodies remain to be determined.
- January 2021
Abstract
Objective
To determine the association between autoantibodies to G-protein-coupled receptors with effect on the cardiovascular system and the cardiac biomarker N-terminal pro-brain natriuretic peptide reflecting heart function in Gravesʼ disease.
Design and Methods
Sixty premenopausal women with Graves’ disease were analyzed for IgG autoantibodies against
β1-adrenergic,
muscarinic acetylcholine type 2 and
angiotensin II type 1 receptors
using enzyme-linked immunosorbent assays based on cell membranes overexpressing receptors in their native conformations.
N-terminal pro-brain natriuretic peptide and heart symptoms were analyzed in hyperthyroidism and after 7.5 months of antithyroid treatment.
Matched thyroid healthy controls were also assessed.
Results
Serum levels of antibodies against the β1-adrenergic and the muscarinic acetylcholine type 2 receptors were higher in hyperthyroid patients than in controls
(median β1-adrenergic receptor antibodies 1.9 [IQR 1.3–2.7] vs. 1.1 [0.8–1.7] μg/mL, P<0.0001; muscarinic acetylcholine type 2 receptor 20.5 [14.0–38.3] vs. 6.0 [3.2–9.9] U/mL, P<0.0001).
These antibodies decreased in euthyroidism (P<0.01), but were still higher than in controls (P<0.01).
Angiotensin II type 1 receptor levels did not differ.
N-terminal pro-brain natriuretic peptide was higher in hyperthyroidism (240 [134–372] vs. <35 [<35–67] ng/L, P<0.0001), normalized after treatment and did not correlate with autoantibodies.
Conclusion
Autoantibodies against the β1-adrenergic and the muscarinic acetylcholine type 2 receptors were increased in Graves’ patients, decreased with treatment, but did not correlate with cardiac function.
However, an autoimmune effect on the heart cannot be excluded in subpopulations, as the functional properties of the analyzed antibodies remain to be determined.