Enterovirus antibody neutralization test in Europe

I

Inez

Messages
89
This is the info I received over a few emails:

Them: Yes, we offer NT testing for the following enteroviruses : Coxsackie A9 , Coxsackie B1-5 , ECHO 4,6,7,9,11,25,30.
For this we need a large white serum tube (10 ml) . non-centrifuged serum. The tube must not contain any additives.

Me: Thank you ver much for the information.
How does the blood sample become serum without a centrifuge?

Them: I apologize - that was perhaps a little unfortunate.
It must be serum. Not whole blood.
Me: Ok, and should the serum be centrifuged?
Them: Not he Serum should not be centrifuged.
 
Hip

Hip

Senior Member
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18,301
Inez said:
I'm so confused
Click to expand...

Serum is the pale yellow liquid that is obtained by centrifuging a fresh blood sample for about 20 minutes.

When you centrifuge a blood sample in a test tube, the blood cells which are heavier move to the bottom of the tube, and the liquid portion of the blood settles on the top of the test tube, and this is the pale yellow serum.

For viral antibody testing, pathology labs want serum, usually around 2 ml. So when you go to a phlebotomy clinic to get a blood sample taken, you just ask them to centrifuge your blood. The phlebotomy clinic will then centrifuge your blood sample there and then, extract the serum, and give you the serum in a new test tube. That test tube can then be sent to the pathology lab for viral antibody testing.
 
Last edited:
I

Inez

Messages
89
Hip said:
Serum is the pale yellow liquid that is obtained by centrifuging a fresh blood sample for about 20 minutes.

When you centrifuge a blood sample in a test tube, the blood cells which are heavier move to the bottom of the tube, and the liquid portion of the blood settles on the top of the test tube, and this is the pale yellow serum.

For viral antibody testing, pathology labs want serum, usually around 2 ml. So when you go to a phlebotomy clinic to get a blood sample taken, you just ask them to centrifuge your blood. The phlebotomy clinic will then centrifuge your blood sample there and then, extract the serum, and give you the serum in a new test tube. That test tube can then be sent to the pathology labs for viral antibody testing.
Click to expand...
Yeah I don't understand how they are asking for serum and also asking that it NOT be centrifuged
 
I

Inez

Messages
89
Right that's what I understood to be the process. I think my lazy language and grammar use has caused me my confusion!
 
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