LDN upregulates the body’s endorphins and met-enkephalins (met enkephalin known as OGF - Opioid Growth Factor), as well as the receptors for the endorphins and OGF to attach onto. The goal is to have LDN antagonising the receptors for around 4 hours.
This then allows for the remaining 20 hours of the day for the upregulated levels of endorphins and OGF to interact with the upregulated receptors. It is this cell interaction that inhibits inflammation, pain and cell proliferation.