Hi @Learner1 .
About the results you've put in. The antibodies against EBNA I'm asking for are IgG (I forgot to put it in, sorry). Anyone who has been infected with Epstein Barr virus has these positive antibodies. But in our disease (as in multiple sclerosis) I think it's much higher than normal...
*snip!*
Another more invasive test is to perform PCR to EBV (does not have to be positive in the blood) on swollen tissue samples, such as the intestinal mucosa or muscle tissue if there is any myopathy. I would use other methods, such as in situ hybridization with a probe that detects EBV-coded RNA (EBER) and is considered the best test for locating latent EBV in tissue samples. O Immuno-FISH combining immunofluorescent staining for surface proteins (using antibodies directly conjugated to fluorochromes) and fluorescent in situ hybridization for EBV DNA. This technique allows simultaneous determination of the type of EBV-infected cells and quantification of the number of EBV copies in the infected cell, which demonstrates that EBV is present not only in B cells (in epithelial cells as well, for example).
.
About the results you've put in. The antibodies against EBNA I'm asking for are IgG (I forgot to put it in, sorry). Anyone who has been infected with Epstein Barr virus has these positive antibodies. But in our disease (as in multiple sclerosis) I think it's much higher than normal...
*snip!*
Another more invasive test is to perform PCR to EBV (does not have to be positive in the blood) on swollen tissue samples, such as the intestinal mucosa or muscle tissue if there is any myopathy. I would use other methods, such as in situ hybridization with a probe that detects EBV-coded RNA (EBER) and is considered the best test for locating latent EBV in tissue samples. O Immuno-FISH combining immunofluorescent staining for surface proteins (using antibodies directly conjugated to fluorochromes) and fluorescent in situ hybridization for EBV DNA. This technique allows simultaneous determination of the type of EBV-infected cells and quantification of the number of EBV copies in the infected cell, which demonstrates that EBV is present not only in B cells (in epithelial cells as well, for example).
.
Paul