I've been trying to get actual data for a diagnosis of CFS, and I'm also just curious and a little worried about how my immune system's doing. I recently got testing done to rule out autoimmune disease. I have hashimoto's and grave's antibodies, but my doctor didn't believe those meant anything, which I know is wrong but I'm not gonna argue on that since I don't think I hypothyroid, at least not severely. Anyway, my general autoimmune screen came back with a positive ANA and positive ribonucleoprotein antibody. Then my doctor ordered more extensive testing to look for autoimmune disease. AntiNuclearAntibody Hep 2 Substrate, which was negative. I'm still concerned, given that my CFS symptoms have worsened over the last few years. I'm debating between going to a local doctor who treats CFS in order to get more testing, getting testing through a private lab, or just asking my doctor for it at my next appointment. The trouble is she doesn't think CFS is treatable, and I'm already scheduled to see a neurologist, so I feel kind of like she might not do more immune testing and not see the point in sending me to an immunologist. I also have a concern about my digestion, especially since celiac runs in my family...so many things to rule out :-/ Sorry for the rambling, my main question is: what is the first line tesing? Total Immunoglobulin? I was interested in NK cell testing since I'm self-diagnosed and it would give me another clue, but I don't think my doctor would test for that and it sounds expensive to get it through a private or research lab but if it would help me be taken more seriously, then I would. Again, thanks for your help everybody.