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Study: Probiotic breaks down gliadin...

Discussion in 'Latest ME/CFS Research' started by dannybex, Aug 27, 2009.

  1. dannybex

    dannybex Senior Member

    Tried to post this a minute ago...not sure what happened.

    2006: De Angelis Maria; Rizzello Carlo G; Fasano Alessio; Clemente Maria G; De Simone Claudio; Silano Marco; De Vincenzi Massimo; Losito Ilario; Gobbetti Marco

    VSL#3 probiotic preparation has the capacity to hydrolyze gliadin polypeptides responsible for Celiac Sprue.
    Biochimica et biophysica acta 2006;1762(1):80-93.

    The native structure and distribution of gliadin epitopes responsible for Celiac Sprue (CS) may be influenced by cereal food processing. This work was aimed at showing the capacity of probiotic VSL#3 to decrease the toxicity of wheat flour during long-time fermentation. VSL#3 (10(9) cfu/ml) hydrolyzed completely the alpha2-gliadin-derived epitopes 62-75 and 33-mer (750 ppm).

    Two-dimensional electrophoresis, immunological (R5 antibody) and mass spectrometry analyses showed an almost complete degradation of gliadins during long-time fermentation of wheat flour by VSL#3. Gliadins non-hydrolyzed during fermentation by VSL#3 were subjected to peptic-tryptic (PT) digestion and analyzed by CapLC-ESI-Q-ToF-MS (Capillary Liquid Chromatography-Electrospray Ionization-Quadrupole-Time of Flight-Mass Spectrometry). Search for several epitopes showed the only presence of alpha2-gliadin-fragment 62-75 at a very low concentration (sub-ppm range).

    Compared to IEC-6 cells exposed to intact gliadins extracted from the chemically acidified dough (control), VSL#3 pre-digested gliadins caused a less pronounced reorganization of the intracellular F-actin which was mirrored by an attenuated effect on intestinal mucosa permeability. The release of zonulin from intestinal epithelial cells treated with gliadins was considerably lower when digested with VSL#3. Agglutination test on K 562 (S) cells showed that the PT-digest of wheat flour treated with VSL#3 increased the Minimal Agglutinating Activity of ca. 100 times.

    Wheat proteins were extracted from doughs and subjected to PT digestion. Compared to PT-digest from chemically acidified dough, celiac jejunal biopsies exposed to the PT-digest from the dough fermented by VSL#3 did not show an increase of the infiltration of CD3(+) intraepithelial lymphocytes. Proteolytic activity by probiotic VSL#3 may have an importance during food processing to produce pre-digested and tolerated gliadins for increasing the palatability of gluten-free products.

    So I wonder if taking this probiotic for a couple of months would have the same positive effect that would allow some w/wheat intolerance to have a piece of BREAD? :)
  2. Noah_Scape



    Ya, I am a big believer in the gut and the bacteria there doing good things.

    That is where all the digestion and absorption happens, and the microflora is allways the key to that... and how we feel, immune stuff, energy, food intolerances.

    So sure, it looks like you can eat bread now that you have the right stuff going on in there.

    It is that way with lactose intolerance - plain yogurt is made with milk, but lactose intolerant people can tolerate yogurt. Must be because of the lactade [sp?] producing bacteria, eh?

    I copied and saved your post - thanks!
  3. dannybex

    dannybex Senior Member

    Thanks Noah...

    Hi Noah,

    Yeah, it would be interesting if they'd do some clinical studies to see if this probiotic would work 'in vivo'...with folks who have gluten intolerance. I'm not sure if it would be safe for folks with outright celiac disease, but for those with mild gluten problems?

    I've read elsewhere of people and their autistic kids taking specific enzymes like No-Fenol and Peptizide who have been able to go of GFCF diets without any problems...as long as they take the enzymes before every meal...so it suggests that correcting these issues...by various methods...may, just may mean that these food intolerance problems aren't necessarily permanent.

    Just wish the enzymes and probiotics weren't so expensive!


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