Stanford News: Davis- Tweak to assay could bolster disease detection

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New research by Ron Davis and the Stanford Genome Center on an improved method to detect certain biomarkers

Full article here
https://med.stanford.edu/news/all-news/2018/02/tweak-to-assay-could-bolster-disease-detection.html

A team of School of Medicine researchers has developed a technique that they hope could more precisely detect diseases or disorders such as cancer or a heart attack.

The technique is an improved method to detect some biomarkers — protein signals in blood or tissues that flag unhealthy or diseased cells. If the biomarker of interest is present, a circle of DNA molecules is created that includes specific proteins, called antibodies, that bind only to the biomarker and a set of DNA sequences that facilitate formation of the circle. If the biomarker isn’t there, no circle forms.

The technique, a type of assay, is based on an existing method called a proximity ligation assay, or PLA, which converts the biomarker into a DNA sequence. The modified assay, called circular-PLA, uses additional DNA molecules to generate a circle, a step that enhances the accuracy of the approach.

The extra stringency is particularly important because existing tests yield too many false positives and false negatives, said said Davis, who is also director of the Stanford Genome Technology Center.

“There’s too much complacency with the existing detection method used in clinics,” Davis said. “I think the medical community needs to push back and just not accept it.”

Davis sees potential for the technique to help detect biomarkers of diseases with high rates of false positives and negatives, such as human papillomavirus or Lyme disease. He also notes that the ability to accurately detect molecules has many potential applications beyond medicine, such as the identification of mold in a building.