The 12th Invest in ME Conference, Part 1
OverTheHills presents the first article in a series of three about the recent 12th Invest In ME international Conference (IIMEC12) in London.
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Soluble CD14 testing

Discussion in 'XMRV Testing, Treatment and Transmission' started by Daffodil, Dec 11, 2011.

  1. Daffodil

    Daffodil Senior Member

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    hi all. the nevada lab will be offering soluble cd14 testing in late january. i think dr. demeirleir has been offering this test for a while.

    has anyone had this test? if so, what did the doctor say it means? did he talk about antibiotics to address this?

    apparently, a high CD14 correlates with bacteria in the blood from leaky gut.

    the immunobilan test does not test for every kind of bacteria so the soluble CD14 test might be more useful.

    i think elevated sCD14 correlates also with a very high TNF-a.

    check out this quote from an article about elevated sCD14 and HIV-associated cognitive impairment:


    Ryan et al. (2001) subsequently confirmed an association between plasma soluble CD14 (sCD14) and cognitive dysfunction in HIV infection. More recently, Ancuta et al. (2008) showed that elevated sCD14 and lipopolysachharide (LPS) levels are associated with HAD. These observations support an independent study which demonstrated that elevated systemic LPS levels and immune activation in chronic HIV infection resulting from microbial translocation are associated with HIV-induced depletion of gut-associated lymphoid tissue (Brenchley et al. 2006). These studies clearly support the hypothesis that systemic immune activation/inflammation triggered by HIV-induced transmicrobial translocation and monocyte activation increases the risk for development of HAND through trafficking of activated monocytes into the CNS.




    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914283/
     
    Isaiah 58:11 likes this.
  2. Sushi

    Sushi Moderation Resource Albuquerque

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    I am PMing you,
    Sushi
     
  3. anniekim

    anniekim Senior Member

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    I'd be very interested In any info on this too. Thank you
     
  4. Daffodil

    Daffodil Senior Member

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  5. filfla4

    filfla4 Senior Member

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    Sushi, my sCD14 is 3,993.00 on a range of 1,430.00 - 2,800.00 ng/ml. Any thoughts/explanation on what it actually means? Any help in understanding this would be welcome!!
     

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  6. Daffodil

    Daffodil Senior Member

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    it means you have leaky gut!
     
    Tito likes this.
  7. naoise

    naoise Senior Member

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    I have a high result too so means leaky gut...interesting as I have huge gut issues!
     
  8. knackers323

    knackers323 Senior Member

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    what labs perform this test? and LPS in blood test?
     
  9. Daffodil

    Daffodil Senior Member

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  10. unicorn7

    unicorn7 Senior Member

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    sCD14 is the soluble version of the CD14-receptor that is also in monocytes. It binds to LPS (lipopolysaccharide = outer wall of gram-negative bacteria). There has been research that has correlated levels of LPS with CD14 levels, so when you have a lot of LPS in your blood, CD14 goes up.
    LPS can only be in your blood when there's pieces of gram-negative bacteria translocating to your blood (through a leaky gut). The same happens in sepsis.

    With me, sCD14 has been consistently going up when symptoms were bad and down when symptoms were better. I was told by KDM that CD14 is the first to go down, when everything starts getting better.
     
  11. Daffodil

    Daffodil Senior Member

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    there is some controversy about the sCD14 test. some say things other than bacterial translocation can make it high
     
  12. Thinktank

    Thinktank Senior Member

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    Source?
     
  13. Daffodil

    Daffodil Senior Member

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  14. nandixon

    nandixon Senior Member

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    Yes, sCD14 cannot be used as a proxy for LPS. A high sCD14 level may simply mean, for example, that one or more inflammatory cytokines are elevated and not that LPS is high. To determine if LPS is really high or not LPS must be measured directly.
     
  15. Daffodil

    Daffodil Senior Member

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    I am going to ask my doc about this
     
  16. Daffodil

    Daffodil Senior Member

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    he said this:

    LPS is unstable and changes from minute to minute. It is ok to use in studies to compare groups, but cannot be used in the follow-up of an individual patient.
    sCD14 gives you the activity of LPS over a longer period.
     
    Helen likes this.
  17. nandixon

    nandixon Senior Member

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    I'm afraid that's making the assumption that an elevated sCD14 is due to an elevated LPS in the first place, which can't be known unless LPS is actually measured at some point in the individual patient. (LPS is only unstable if it's not handled and stored properly.)
     
  18. Daffodil

    Daffodil Senior Member

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    @nandixon well this is from the world's best CFS doctor so i dont know what to tell you
     
    JadeD likes this.

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