The 12th Invest in ME Conference, Part 1
OverTheHills presents the first article in a series of three about the recent 12th Invest In ME international Conference (IIMEC12) in London.
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Lyme Disease: Dust mites carry Bartonella

Discussion in 'Lyme Disease and Co-Infections' started by Ecoclimber, Jun 30, 2012.

  1. Ecoclimber

    Ecoclimber Senior Member

    Bacterial 16S ribosomal DNA in house dust mite cultures.

    Valerio CR, Murray P, Arlian LG, Slater JE.

    Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD, USA.


    Allergen extracts prepared from Dermatophagoides farinae contain significantly more endotoxin than Dermatophagoides pteronyssinus extracts, and extracts from both mite extracts contain more endotoxin than pollen extracts. Attempts to culture bacteria from mite cultures have failed to establish the sources of the endotoxin.


    To determine the bacterial sources of endotoxin in mite extracts.


    Live mites of both species were obtained from 2 sources, DNA was extracted from the mites, and DNA encoding bacterial 16S ribosomal RNA was amplified by using specific primers. The amount of bacterial DNA in each mite DNA sample was determined by quantitative PCR using an internal standard, and sequence homologies were determined from amplifications performed by using a high-fidelity DNA polymerase.


    DNA from D farinae appeared to contain between 11-fold and 24-fold more 16S ribosomal gene copies than the genomic DNA from D pteronyssinus (P < or = .003). Sequence analysis indicated the dominant presence of at least 3 phylogenetic clusters of Bartonella species (henselae, quintana, vinsonii, and grahamii), as well as uncharacterized alpha-proteobacteria, from both D farinae and D pteronyssinus. In a few clones, sequences from Escherichia coli, Pseudomonas species, and Acinetobacter species were also identified.


    House dust mite DNA contains evidence of Bartonella and other Gram-negative species. These Gram-negative species are likely to be the sources of the endotoxin found in mite allergenic extracts.
    PMID:16337462[PubMed - indexed for MEDLINE]

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    merylg and Merry like this.
  2. *GG*

    *GG* senior member

    Concord, NH
    Not a new finding by any means. Thanks for posting. I think I might have this, will have bloodwork soon. I probably have dust mites, not a clean freak.


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