@ Brenda: Wow, that blog is...rich with information.
My understanding of what they were saying was:
LB Broth may not be a perfect growth medium for E. coli. As with pretty much any growth medium, what this means is that E. coli will grow "normally" until it runs out of the necessary and preferred nutrients. Like humans, when runs out of necessary nutrients (they discussed magnesium) it will continue to grow for a while, but it will not be as healthy. Also, when it runs out of the nutrients it prefers (they suggested that it might prefer to use some amino acids over others) it will use other ones that it does not like as much. Again, this will probably effect its growth.
This is seen with E. coli in the pattern of growth, where it reproduces quickly for some time until it runs out of what it wants, and...I think that basically they see less growth of E. coli in the petri dishes, and even a shrinkage of it after enough time.
My thought is that for us, we do not necessarily need E. coli nissle to gobble up every bit of what we give it, and to that end perhaps both the soy-milk method from Dr. Myhill's web site and the LB broth method might work. On the other hand, if we do allow it to grow as much as it possibly can (thus pushing it into territory where one or a few nutrients are lacking), will it spring back once deposited in our guts and given all the goodies therein?
How much do you ferment yours,
@donovank730?
