EGCG inhibits adhesion and migration of peripheral blood B cells

[adreno]

Green tea, anyone?

Immunopharmacol Immunotoxicol. 2011 Jun;33(2):391-7. doi: 10.3109/08923973.2010.522195. Epub 2010 Oct 11.
Catechin inhibits adhesion and migration of peripheral blood B cells by blocking CD11b.

Kawai K, Tsuno NH, Kitayama J, Sunami E, Takahashi K, Nagawa H.
Source

Department of Surgical Oncology, Faculty of Medicine, University of Tokyo, Tokyo, Japan. kz-kawai@mvd.biglobe.ne.jp
Abstract

Context: Previously, we demonstrated that CD11b is expressed on peripheral blood memory B cells, and it plays an important role in the migration of B cells. And epigallocatechin gallate (EGCG), a bioactive component of green tea, by binding to CD11b, expressed on CD8(+) cytotoxic T cells, inhibited their migratory ability, one possible mechanism of the antiallergic activity of EGCG. Objective: Here, we investigated whether EGCG also affected CD11b expressed on B cells, similar to cytotoxic T cells. Materials and methods: Isolated peripheral blood CD19(+) B cells were treated with EGCG and the change in the expression of CD11b was analyzed using flow cytometry. The effects of EGCG on the ability of B cells to adhere to and to transmigrate through the endothelial cell layer were evaluated using the transwell assay. Results: EGCG significantly suppressed the apparent expression of CD11b on B cells, in the flow-cytometric analysis, and this apparent suppression was speculated to be dependent on the competitive binding of EGCG to CD11b. EGCG also significantly suppressed CD11b-mediated migration and adhesion of B cells to endothelial cells. Discussion and conclusion: EGCG has a strong suppressive activity on the adhesive and migratory abilities of peripheral blood B cells. This suppressive activity was mediated by the binding of EGCG to CD11b on B cells, and the consequent suppression of B-cell extravasation to the extravascular space. Because B cell plays an important role in the humoral immunity, EGCG could be a promising drug for the prevention and/or treatment of allergic and/or autoimmune diseases.
PMID:

20936888

 

[GhostGum]

Still one of the best all round supplements/substances there is. I always mean to start making some green tea, since it is so much better taken as a tea but never get around to it. Some people I know make a huge batch of it, turn it into an ice tea so they can store it in the fridge, probably the way to go; might taste better with an added electrolyte mix or with some d-ribose as well.

Thanks for the reminder

 

[Crux]

adreno,

Thanks for this thread, I've become a bit obsessed with EGCG, for cancer prevention, treating autoimmune conditions, etc.

http://cancerpreventionresearch.aacrjournals.org/content/4/3/396.full

http://www.sciencedaily.com/releases/2007/04/070419140910.htm

It has also been used for weight loss.

 

[Hip]

Some EGCG (epigallocatechin gallate) notes:

• If you are taking large doses of EGCG, it may be a good idea to take supplemental folic acid, as EGCG can deplete folate.

• The plasma half-life of EGCG is around approximately 4 hours, so this is a supplement you'd need to take 3 or 4 times daily.

• A cheap way to obtain EGCG is by buying it in bulk powder. If you are going to take a lot, then try to find a decaffeinated EGCG powder.

 

[Crux]

Thanks Hip;
It's good for people to become aware of its properties.