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Live slides from participants with rheumatoid arthritis, chronic fatigue syndrome, and prosthetic joint infection provide direct visual evidence that infectors are present in blood cells and thriving within plasma is clear.
@alicec Is next generation sequencing likely to show a bacterial infection like this?
Apparently not. Indeed I think it's quite amazing what NGS doesn't show.
Note that this paper is not peer reviewed or published
Is next generation sequencing likely to show a bacterial infection like this?
The question is probably not if, but rather when, WG-NGS (whole genome next generation sequencing) will become a routine test in diagnostics of infectious diseases. This development will require improvement in sample preparation, availability of sequencers in central laboratories and validated pipelines for read sorting and taxonomic assignation. There is no doubt that such an opportunity will sooner than later profoundly change the routine laboratory practice together with the means of conducting microbiological diagnosis.
Apparently not. Indeed I think it's quite amazing what NGS doesn't show.
Not only is it not peer reviewed, it presents no methods so reliability of results can't be scrutinised, while results are given as a perfunctory summary - again they can't really be scrutinised.
In short it is not a scientific paper with any credibility, nor does the author appear to have any scientific background that I could find.
Soft Cell Biological has developed a patent-pending protocol to culture and examine hidden bacteria in the circulatory system. Our ultimate goal is to reveal the link between these bacteria, autoimmune disorders, and recurrent infection.
Preliminary testing reveals that donors suffering from chronic fatigue, fibromyalgia, arthritis, and anemia carry high concentrations of L-form bacteria in their blood.
Despite the medical community’s recognition that bodies attack themselves, the source of this process is mysterious—a source we have potentially discovered. In lacking cell walls, these bacteria are able to invade red blood cells, traveling freely throughout the body and eventually taking any cell within as a host. The ability to culture L-form bacteria from two drops of blood reveals new avenues toward solving these problems.
Source: About | Soft Cell Biological Research
In Soft Cell Biological’s laboratory formed in conjunction with Dixie State University, we have developed new methods for the growth of L-form bacteria isolated from synovial, capillary blood, and whole blood samples. Currently, using 16S rRNA sequencing, we have successfully identified several new species of bacteria from humans that appear to act as latent infectors in some populations.
Source: here
John Brent Hunt, is the founder and CEO of the company Soft Cell Biological Research:
It does seem a little strange that this discovery has not been published in a peer review scientific journal.
This is from his paper on the subject. Note that this paper is not peer reviewed or published and is only available to download as a word document from ResearchGate:
https://www.researchgate.net/profile/Brent_Hunt/publications
The ResearchGate "paper" and the website rang a lot of alarm bells for me. I'd like to see much more evidence before I take it seriously.
It’s not unlike Einstein’s great and well known equation E=MC2 [that 2 is supposed to be superscript!]: if he had merely written E=MC, it would have equated to nothing more than a group of letters and a sign without the missing factor. I believe that to be the case for L-form and the significance of any treatment, antibiotic, or surgical procedure developed from this point forward that does not consider all factors—namely, the L-factor.
So, getting back to the subject of this thread, l-form bacteria, based on what I've seen so far, I'd prefer to rely on WG-NGS to determine prevalence in blood or other biological samples.