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This was posted on ERV:
http://scienceblogs.com/erv/2011/11/xmrv_and_chronic_fatigue_syndr_30.php#comment-5915108
http://antiviralresistance.org/abstract26_2011.pdf
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So if I get it right, Coffin and his group discovered that Lombardi et al. suffered from two types of contamination (XMRV from cell lines and mouse genomic DNA), as predicted by various other studies in Retrovirology?
http://scienceblogs.com/erv/2011/11/xmrv_and_chronic_fatigue_syndr_30.php#comment-5915108
From the 12th Annual Symposium on Antiviral Resistance, held in Hershey PA on Nov. 8, 2011:
Multiple Sources of Contamination in Samples from Patients Reported to Have XMRV Infection
M.F. Kearney, J. Spindler, A. Wiegand, W. Shao, E.M. Anderson, F. Maldarelli, J.W. Mellors, S. H.
Hughes, S.F.J. Le Grice, and J.M. Coffin
Xenotropic murine leukemia virus (MLV)-related retrovirus (XMRV) was reported to be associated with prostate cancer by Urisman, et al. in 2006 and chronic fatigue syndrome (CFS) by Lombardi, et al. in 2009. To investigate this association, we independently evaluated plasma samples from 4 patients with CFS reported by Lombardi, et al. to have XMRV infection and from 5 healthy controls reported to be XMRV uninfected. We also analyzed viral sequences obtained from supernatants of cell cultures reported to contain XMRV after coculture with clinical samples from 9 patients. A qPCR assay capable of distinguishing XMRV from endogenous MLVs showed that the viral sequences detected in the CFS patient plasma matched endogenous MLVs and not XMRV. Single-genome sequences (N=89) from CFS patient plasma were indistinguishable from endogenous MLVs found in the mouse genome that are distinct from XMRV. By contrast, XMRV sequences were detected by qPCR in 2 of the 5 plasma samples from healthy controls (sequencing of the qPCR product confirmed XMRV not MLV). Single-genome sequences (N=234) from the 9 culture supernatants reportedly positive for XMRV were indistinguishable from XMRV sequences obtained from 22Rv1 and XMRV-contaminated 293T cell-lines. These results indicate that MLV DNA detected in plasma samples from CFS patients was from contaminating mouse genomic DNA and that XMRV detected in plasma samples from healthy controls and in cultures of patient samples was due to cross-contamination with XMRV (virus or nucleic acid).
http://antiviralresistance.org/abstract26_2011.pdf
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So if I get it right, Coffin and his group discovered that Lombardi et al. suffered from two types of contamination (XMRV from cell lines and mouse genomic DNA), as predicted by various other studies in Retrovirology?