Hi, I understand that this thread is discussing the minutiae of PCR so my comment will go off at a tangent, but I really feel that we should have more faith in the WPI and in people like Alter, Ruschetti and Mikovits. From long experience in the ME world I have noticed that IT IS ALWAYS THE BEST PEOPLE who are prepared to listen to us and to try to help us. It is easy to judge with the collective voice but it takes an independence of mind to look at the patient in front of you and ask yourself some fundamental questions. This makes for a better scientist. It might sound odd but there are moral and ethical issues here and those who try to help us are better people - I have seen this time and time again. What I mean is that those who have believed us and accepted our story that we are ill, and have not made the easy judgement, and the safe one, will always be better scientists and will have the commitment to find the cause of our illness. What has this spanish guy ever done for us? Why should we be influenced by him when we have better, more committed scientists on our side? Has he ever thought much about ME/CFS? What does he care about our illness? Negative studies will always be easier to do where the science is new. To really think and look for an answer takes care and understanding and commitment. This is what leads to good science, science that will look with understanding for the right answer, which means to look at the patient, not just at what goes on in the lab. I stick with the WPI.