This has happened for a second time recently. About 3 weeks ago after supplementing methylfolate 300mcg/day for 2 weeks with no b12 I was feeling a bit agitated so I took 20mg of Niacin and felt it took the edge off, I wanted to see how I reacted to more Niacin and took 150mg spread out through the next day. I ended up feeling severely depressed for days with low energy. OK, I understand the part of soaking up methyl donors, but why would I be plunged into a deep depression? I couldn't find any information of this happening to anyone else. And I have never experienced this before with Niacin. I cycled through a bunch of supplements zinc, magnesium and eventually when I tried b12 it completely stopped the depression, I didn't feel great but was relieved that I felt neutral. So fast forward two weeks, I was introducing methylfolate slowly, taking about 200mcg for a few days while taking 3mg mb12 and 3mg ab12. Until yesterday when I took 300mg around midday followed by another 200mg at night. Later that night I was very anxious and agitated, had a restless nights sleep and also head pressure. It lasted through to today when I decided to take 25mg of Niacin, those symptoms mostly subsided but was again replace with a depression.... from 25mg of Niacin. That's only slightly more than the RDA! What's going on here? Is it straight up b12 deficiency? methyl trapping? Maybe B out of balance? Or did the Niacin use up Sam-e? Let's say for example I had b12 deficiency and taking folate and niacin was exacerbating the problem. Wouldnt the 25mg of mB12 + 20mg of aB12 I have been taking over the last 2 weeks take on the miniscule amount of folate in comparison? Also for the last 3 weeks I have been experiencing waking up with very dry eyes and completely dry mouth. How does this fit in, in terms of methylation?