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Urine-Based XMRV testing?

Discussion in 'XMRV Testing, Treatment and Transmission' started by Daffodil, Sep 8, 2010.

  1. Daffodil

    Daffodil Senior Member

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  2. George

    George waitin' fer rabbits

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    Go Daffodil!

    Great find

    From the site

    Urine-Based XMRV RT-PCR Detection Kit (Cat# 34700 )

    A ready-to-use system for the isolation and detection of XMRV viral RNA from urine using end-point RT-PCR (

    * Rapid isolation of high quality viral RNA from urine
    * Contains a ready-to-use 2X RT-PCR Master Mix
    * High sensitivity and specificity
    * Includes an isolation control and a PCR control
    * Primer set and controls also available separately
    * Ideal for use in:
    1. Surveillance of Drug Resistant Pathogens
    2. Epidemiologial Studies
    3. Field Surveillance of Pathogens
    4. Surveys

    Xenotropic murine leukemia virus-related virus (XMRV) belongs to the family Retroviridae and the genus gammaretrovirus. The virus was first described in 2006 and has since been isolated from human biological samples. XMRV has a single-stranded RNA genome that replicates through a DNA intermediate. The virus gets its name due to its close relationship with the murine leukemia viruses (MuLVs). The viral genome is approximately 8100 nucleotides in length and is 95% identical with several endogenous retroviruses of mice. While gammaretroviruses have well-characterized oncogenic effects in animals, they have not been shown to cause human cancers. However, XMRV was recently discovered in human prostate cancers and is the first gammaretrovirus known to infect humans. In addition to prostate cancer, a possible association with chronic fatigue syndrome has been reported, however it has yet to be established whether XMRV is a cause of this disease. The causal role of XMRV in cancer has yet to be established and the virus does not appear to be capable of transforming cells directly.

    Norgen’s Urine-Based XMRV RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of XMRV from urine. First the kit contains components for the rapid isolation of total RNA, including viral RNA, from the urine samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second the kit contains XMRV RT-PCR Master Mix and controls for PCR Amplification. The amplified PCR products are then detected using agarose gel electrohporesis. Alternatively, detection can be performed by real-time PCR using melt curves (Detection). Please see the flowchart to the right.

    The XMRV Master Mix contains reagents and enzymes for the specific amplification of a 300 bp region of XMRV. In addition, Norgen’s Urine-Based XMRV RT-PCR Detection Kit contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples. the XMRV RT-PCR Primer Set and Controls are also available separately for end-point RT-PCR detection.

    FEATURES AND BENEFITS

    * Non Invasive, Non Infectious Sample Source – Urine is not infectious for most human pathogens.
    * Isolate High Quality, Inhibitor-Free RNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated RNA.
    * Rapid Procedure - Isolation and detection of XMRV in less than 3 hours.
    * Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap
    * High Sensitivity and Specificity - The limit of detection is less than 2000 VP/mL of urine
    * Purchase Primer Set and Controls Separately - The XMRV Primer set as well as the positive control and negative control can be purchased separately



    I like that accessories are sold separately. (big grins) Batteries not included????
     
  3. shannah

    shannah Senior Member

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  4. julius

    julius Watchoo lookin' at?

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    This looks like its for research, not general public. Is that right?

    But what a huge thing if this works. Three hours!!! Nice!
     
  5. Berthe

    Berthe Senior Member

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    Is this perhaps the test dr. De Meirleir is throwing in at his pee-party (read; presentation) at the 22nd of september in Belgium. The presentaton costs 15 Euro for not members and includes one free drink and one free urine-test. Lots of patients in one room, great researchmaterial. It is all coming together.

    I'm going! He can count me in.

    Love,
    Berthe
     
  6. camas

    camas Senior Member

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    Seems I remember Mikovits saying that urine would be the perfect pH for XMRV. My illness began 30 years ago with a bladder infection that led to interstitial cystitis. My IC has never completely resolved, but was always at its worst when my progesterone levels were highest. As I've aged and these levels have naturally began to lessen, my IC has improved. Was progesterone feeding XMRV?

    I don't remember if they found XMRV in the bladders in the primate study, but if they can find it in urine, doesn't that make it a likely candidate? It's feeling like the pieces are starting to fall together.
     
  7. Boule de feu

    Boule de feu Senior Member

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    Is this really for real?

    Yesterday, experts were debating if they could find XMRV or not...
    and now this? How is it possible?
     
  8. Daffodil

    Daffodil Senior Member

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    shannah...lol i dont know...i was googling and must have thrown in a word that i hadnt used before and this came up.

    wonder what the WPI thinks about this test.
     
  9. Boule de feu

    Boule de feu Senior Member

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    I would love to know, too!
    Can someone send this to Judy and see what she thinks of it?
     
  10. Sunshine

    Sunshine Senior Member

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    Hi.

    XRMV DNA in the urine is no real surprise biologically, as urine is filtered blood and XMRV is dectable in the blood. It is however a surprise to CFS patients as they didn't know.

    Urine is a body fluid and retroviruses are found in body fluids, hence HIV is sexually transmitted.
     
  11. Daffodil

    Daffodil Senior Member

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    i dont think HIV can be transmitted via urine.

    i am going to ask my connection to forward this to judy..i dont think she opens my mail anymore lol
     
  12. George

    George waitin' fer rabbits

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    They tweeted about the urine based XMRV detection assay yesterday

    But what chaps my doggy butt is the fact that the assay already exists. Yesterday's conference was like months behind what is actually going on.

    While retroviruses can be "excreted" via urine or feces it's not "infectious" in that form.
     
  13. Daffodil

    Daffodil Senior Member

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    "what chaps my doggy butt" LMAOOOOO didnt think i would laugh today
     
  14. George

    George waitin' fer rabbits

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    (big grins)

    Here's some interesting stuff what do ya'll think.

    and this one

     
  15. nventor

    nventor

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    I can't believe this site is not on fire with the buzzzzzzz about a urine test for XMRV, what's up with that?

    Roger
     
  16. Otis

    Otis Señor Mumbler

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    Here's some doggie Chapstick

    Well, they tweeted about the presence in urine but not this assay.

    I sure would like to know the pedigree of these assays that seem to pop up out of the blue.
     
  17. kat0465

    kat0465 Senior Member

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    has anybody figured out if it's available to the general Public?? or just docs?
    and if we can buy a test, how much is it???

    I would be thrilled to just pee in a cup and find out if im Positive!

    Kat
     
  18. Otis

    Otis Señor Mumbler

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    Wish I could be there.

    Just remember:
    Left hand free drink, right hand sample cup
    Left hand free drink, right hand sample cup
    Right hand free drink, left hand sample cup - DOOOH

    :Retro smile:
     
  19. George

    George waitin' fer rabbits

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    Not available to the public and you have to have a PCR machine to actually use it. Doesn't look like it's available to Doctors but rather Labs only. At least that's what I get from the web site.

    Good point Otis, about the pedigree. Daffodil has e-mailed Dr. Mikovits and we will hopefully hear back soon about the validity of this test. It's certainly doable in that it has been done with both HTLV and HIV but don't know if it's for real or not. Anybody else want to e-mail anybody else, like Dr. Bateman or Dr. Singh and ask that would be cool too.
     
  20. Forebearance

    Forebearance Senior Member

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    It would sure be better than giving, say, a sample of brain tissue!
     

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