I've suspected the NOS mutation long before my results revealed I am +/+ for it. So, for the last three weeks (I think) I've been taking various aminos to support the NOS pathway, despite it being a lower priority mutation according to Yasko. My reason for doing this is that I have so many toxins, and ammonia, in my system that I can't just let them sit there. As with every other supplement bar d-ribose (which helps very slightly with energy - I'd say about 10% maximum) I'm only feeling worse, though; although this could be for any number of reasons, such as the CFS not responding, other supplements interacting with things, hypothyroidism, stress levels etc. Anyway, I've based my NOS treatment on accounts I've read in this forum. My memory is sketchy, but suffice it to say I'm taking them all because of their relationship with one another; for example I'm taking supplement y because supplement x increases the need for it. So, here's what I'm doing (note that I am taking the recommended amount given on the packet) daily; either first thing in the morning, or between breakfast and lunch l-citrulline A-AKG l-ornithine l-lysine BCAAs Every 3 days I take 3 sprays of HHI Amino Assist, although this contains methionine, which I'm deficient in but I think supposed to be avoiding (forgtten why, silly me), in addition to tyrosine and tryptophan, which my UEE/UAA showed high excretions of (I am taking T4 and T3 thyroid medication, which does not appear to be getting absorbed, which may account for the high secretions of tyrosine. I was also trialing the SSRI sertraline at the time, which may account for the elevated tryptophan). I am also taking l-glutamine because of leaky gut, despite the controversy. I'm not sure if that has any mitigating factors on the other aminos. So, what does anyone think? Is this too much? Is it too soon to say? Would it be worth my while getting another UAA/UEE? Any help and advice is greatly appreciated, thanks.