Professor & patients' paper on the solvable biological challenge of ME/CFS: reader-friendly version
Simon McGrath provides a patient-friendly version of a peer-reviewed paper which highlights some of the most promising biomedical research on ME/CFS ...
Discuss the article on the Forums.

Sulfite-Sulfate Conversion and HydroxyB12

Discussion in 'Detox: Methylation; B12; Glutathione; Chelation' started by picante, Jan 24, 2015.

  1. picante

    picante Senior Member

    Messages:
    763
    Likes:
    878
    Helena, MT USA
    I've run across this on Heartfixer and several forums:

    "Sulfite is neurotoxic. Sulfite will be over produced by the CBS up regulation, and then requires conversion in to the less toxic sulfate molecule by the enzyme Sulfite Oxidase (SUOX). SUOX can easily be overwhelmed. Molybdenum is required for SUOX function, and is typically depleted in CBS (+/+) or (+/-) individuals. Molybdenum supplementation (3 drops or 75 mcg of e-lyte Molybdenum twice a day), Boron 3 mg/day, Vitamin E succinate 400 IU/day, and hydroxy-B12 2000 mcg/day are also utilized to speed up SUOX activity."

    Several things are happening that point to a sulfite/ammonia problem for me. Now I've just taken a bit of hydroxyB12 (1,000 mcg) and my brain fog / hypoxia is clearing. Same thing last night. Before that, I was doing the deadlock quartet.

    This fog I keep getting was not a problem until I started methylation. It is very much like the grogginess that always came on at high altitude (11,000') back in my hiking days, which would be hypoxia. I can't keep my eyes open -- that kind of grogginess.

    Why would hydroxyB12 help with this, does anyone know?
     
    helen1 and Gondwanaland like this.
  2. picante

    picante Senior Member

    Messages:
    763
    Likes:
    878
    Helena, MT USA
    Here are my CBS snps, which told me nothing. The 3rd column is the risk allele, the fourth column is my alleles.

    CBS A13637G rs2851391 T no call
    CBS A360A rs1801181 A no call
    CBS C19150T rs4920037 A GG -/-
    CBS C699T rs234706 A GG -/-
    CBS N212N rs2298758 A not tested
     
  3. picante

    picante Senior Member

    Messages:
    763
    Likes:
    878
    Helena, MT USA
    And SUOX, which I had to pull from my raw data:
    rs11171718 GG
    rs705703 CC
    i5000977 AA
    i5000976 no call
    i6028286 CC

    No clue which ones are the risk alleles Sterling's app doesn't list these.
     
  4. Gondwanaland

    Gondwanaland Senior Member

    Messages:
    4,607
    Likes:
    3,594
    DH and I are CC for rs2851391
    I'm AG and DH AA for rs1801181
    I am CT for rs705703
    DH and I are AA for i5000976
    And the same as you for the other ones.
     
    picante likes this.
  5. ahmo

    ahmo Senior Member

    Messages:
    4,349
    Likes:
    6,549
    Northcoast NSW, Australia
    @picante...Thank You! My SUOX match your 1st 2. How did you do that? I put SUOX, or the rs numbers, into Promethease, and get a blank...It's my SNPtips add-on in Firefox that lights up the same as your SNPs. Here's something definitive for me re my sulfur issues!:thumbsup::)
     
    picante likes this.
  6. picante

    picante Senior Member

    Messages:
    763
    Likes:
    878
    Helena, MT USA
    You're very welcome, dear ahmo. I log into my 23andme account, click on "browse raw data" and enter the gene in the gene box (or the rs# in the rs box).

    Definitive?? I have no clue whether these snps are a problem. For all I know, we're both having sulfur issues because the tea we buy is contaminated with sulfites.
    So have you tried hydroxyB12 when you get the symptoms?
     
  7. ahmo

    ahmo Senior Member

    Messages:
    4,349
    Likes:
    6,549
    Northcoast NSW, Australia
    I don't drink tea, don't eat anything w/ sulfites. Not Hydroxy, but AdB12 has been helpful. And ALCAR, many of Martin Pall's suggestions. Browse raw data...thanks for that. ;)
     
    picante likes this.
  8. picante

    picante Senior Member

    Messages:
    763
    Likes:
    878
    Helena, MT USA
    I guess this is why they say that taking B6 can make a transsulfuration upregulation worse. Maybe this is related to your B6 sensitivity, @ahmo.

    Abstract
    "The first committed step of transsulfuration is catalyzed by cystathionine beta-synthase (CBS), a known pyridoxal 5'-phosphate (PLP) enzyme. The inferred amino acid sequences of rat liver CBS and rat liver hemoprotein H-450 are identical. We now confirm the presence of heme b in rat and human liver CBS. Heme almost entirely accounts for the visible spectrum of CBS rather than PLP. Human CBS, expressed in Escherichia coli, acquires heme b from the host bacteria. delta-Aminolevulinate supplementation during bacterial growth increases both the heme saturation and the specific activity of the homogeneous enzyme more than 3-fold. 1 mol of the 63-kDa CBS subunit binds 1 mol of each (heme and PLP). The presence of heme is required for PLP binding, and the amount of PLP bound is limited by the heme content. Removal of PLP, but not heme, from CBS is reversible. These findings suggest that heme is functionally incorporated into CBS only during protein folding. This report describes the first instance of an enzyme that depends upon both heme and PLP for its function."

    http://www.ncbi.nlm.nih.gov/pubmed/7929220
     
    Gondwanaland and ahmo like this.

See more popular forum discussions.

Share This Page