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Self test for Th1/Th2 balance

Discussion in 'Latest ME/CFS Research' started by Nielk, Feb 17, 2012.

  1. Nielk

    Nielk

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    Test of immune activity in ME/CFS indicates it's a condition associated with overactive Th2 response
    ProHealth.com
    February 16, 2012
    Article:
    Self-test monitoring of the Th1/Th2 balance in health and disease with special emphasis on chronic fatigue syndrome/myalgic encephalomyelitis
    Source: Journal of Medical Laboratory and Diagnosis, Feb 2012

    By Chris Roelant and Kenny De Meirleir

    [Note: Th (T helper) cells trigger and direct the immune response. Th1 and Th2 are different subsets that muster response to different challenges. Broadly, Th1 musters cellular immunity, and Th2 is involved in humoral immunity (noncellular components of blood such as plasma & lymph) and mucosal immunity and allergy. Different diseases may dysregulate the Th1-Th2 balance: be either Th1 or Th2 mediated/dominant. This quick urine test for Th1/Th2 activity allows evaluation by resulting color of whether a treatment strategy such as antioxidants, nutraceuticals or drug is working to restore balance. And tests of more than 450 chronic fatigue syndrome/ME patients suggests ME/CFS involves heightened Th2 activity.]

    To read the full text of this article free, CLICK HERE.

    Abstract: A simple self-test principle is described which allows patients to evaluate their Th1/Th2 balance repeatedly over short periods of time to follow-up the effects of taking pre- and probiotics, neutraceuticals, drugs or any other therapeutic strategy to balance Th1/Th2 status.

    By analyzing a large number of first morning urine samples obtained from individuals with medical conditions associated with an overactive Th2 arm (ulcerative colitis, autism, blastocystis, mercury poisoning and viral infection), a reaction principle was discovered that uses a redox-active colorimetric substrate changing color upon reaction with metabolites present at high concentration in the urine samples of Th2-shifted individuals.

    The development of color is time-dependent and quantitative.

    Moreover, 75% of urine samples obtained from chronic fatigue/myalgic encephalomyelitis patients produced a time-dependent and quantitative change of color compared to only 4% of the controls (perfectly healthy population), providing evidence that chronic fatigue syndrome/myalgic encephalomyelitis is a condition associated with an overactive Th2 arm.

    Source: Journal of Medical Laboratory and Diagnosis, Feb 2012. By Roelant C, De Meirleir K. Protea Biopharma N.V., Neder-Over-Heembeek, Belgium; Department of Human Physiology, Free University of Brussels (VUB), Brussels, Belgium. [Email: croelant@proteabiopharma.com]
     
    Little Bluestem likes this.
  2. mellster

    mellster Marco

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    Awesome - thanks! Hopefully this can be ordered soon and we can so some tally of whether this hypothesis holds up (for PR members at least).
     
  3. mellster

    mellster Marco

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    Wait, I went to their website and it shows the usual H2S urine test. I took that one and it was negative, so no news here..
     
  4. Nielk

    Nielk

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    Mellster,

    Where did you see that? I was just going to ask if anyone knows if this self testing is available yet?
     
  5. Dreambirdie

    Dreambirdie work in progress

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    Yes, Mellster, please post a link to the website. Thanks
     
  6. mellster

    mellster Marco

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    I googled proteopharma and they have a cfs/me urine test on their website that looks just like the one in the article (pdf version available for download) you referenced - same colors and stuff. And this is their H2S test which I took some time ago and there was no h2s detected (at least not within the given time interval). http://www.proteabiopharma.com/page/cfs-me.php
     
  7. Dreambirdie

    Dreambirdie work in progress

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    This lab--Proteabiopharma looks like it's in Belgium right? Is there somewhere in the US that does the same test?
     
  8. Emma

    Emma

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    It's the same old H2S test from De Meirleir, isn't it? The one that was launched at a London conference a few years ago, and which other researchers/physicians seem less than enthusiastic about?
     
  9. mellster

    mellster Marco

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    Yeah, looks like it - it didn't show positive for me (though I had plenty of gut issues at that time). But maybe if enough people on this forum could take this simple and relatively inexpensive test (even if shipped from Europe), maybe we can get a better feel about the predictive value of H2S in urine for CFS.
     
  10. Nielk

    Nielk

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    Are you syre that this is the same test? I agree that the pictures look the same as far as how to test but, it says that this test is for metabolites. Is this the same as th1-th2 testing?
     
  11. mellster

    mellster Marco

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    No, I am not sure - would be nice if this was a different, more specific test for Th1/Th2 imbalance. I guess we'll see, maybe somebody can shoot them an email and ask :)

     
  12. snowathlete

    snowathlete

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    Id definately take this test if it is a new one.
    The old one doesnt sound reliable.
     
  13. Kate_UK

    Kate_UK Senior Member

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    It says

    So if you do this test, and it changes colour, you should not drink green tea?
     
  14. richvank

    richvank Senior Member

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    Little Bluestem likes this.
  15. Kate_UK

    Kate_UK Senior Member

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    Hi Rich - do you know if this is the same test as the neurotoxic metabolite test that some of us did a couple of years ago?
     
  16. Nielk

    Nielk

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  17. Sushi

    Sushi Moderator and Senior Member Albuquerque

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    This seems to be an expansion on a test that has been available for some time. Follow the link for the full article.

    http://www.academicjournals.org/JMLD/PDF/pdf 2012/Feb/Roelant and De Meirleir.pdf

    Self-test monitoring of the Th1/Th2 balance in health
    and disease with special emphasis on chronic fatigue
    syndrome/myalgic encephalomyelitis

    1 Chris Roelant and 2 Kenny De Meirleir

    1 Protea Biopharma N.V., De Tyraslaan 111, 1120 Neder-Over-Heembeek, Belgium.
    2 Department of Human Physiology, Free University of Brussels (VUB), Brussels, Belgium.

    Accepted 18 October, 2011

    A simple “self-test” principle is described which allows patients to evaluate their Th1/Th2 balance
    repeatedly over short periods of time to follow-up the effects of taking pre- and probiotics,
    neutraceuticals, drugs or any other therapeutic strategy to balance Th1/Th2 status. By analysing a large
    number of first morning urine samples obtained from individuals with medical conditions associated
    with an overactive Th2 arm (ulcerative colitis, autism, blastocystis, mercury poisoning and viral
    infection), a reaction principle was discovered that uses a redox-active colorimetric substrate changing
    color upon reaction with metabolites present at high concentration in the urine samples of Th2-shifted
    individuals. The development of color is time-dependent and quantitative. Moreover, 75% of urine
    samples obtained from chronic fatigue/myalgic encephalomyelitis patients produced a time-dependent
    and quantitative change of color compared to only 4% of the controls (perfectly healthy population),
    providing evidence that chronic fatigue syndrome/myalgic encephalomyelitis is a condition associated
    with an overactive Th2 arm.
    Key words: Th1/Th2 balance, urine samples, self test, chronic fatigue syndrome/myalgic encephalomyelitis
     
  18. Sushi

    Sushi Moderator and Senior Member Albuquerque

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    Reading this I was interested to see that, according to this article, green tea will shift your immune system toward Th2. As was pointed out, since most of us already have a shift toward Th2, this is an example of inadvertently using a supplement/food that could exacerbate an underlying immune problem.

    Out goes the green tea! :(

    Sushi
     
  19. Snow Leopard

    Snow Leopard Senior Member

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    I heard a rumour of an independent group that wanted to try and replicate this, but gave up early after they found poor specificity in their pre-trial testing.
     
    oceanblue likes this.
  20. Chris

    Chris Senior Member

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    Sushi, thanks for finding and posting this! Like you, I shall regret that green tea seems to be on the wrong side of our case--and I just bought some good macha tea because I like it! Best, Chris
     

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