Review: 'Through the Shadowlands’ describes Julie Rehmeyer's ME/CFS Odyssey
I should note at the outset that this review is based on an audio version of the galleys and the epilogue from the finished work. Julie Rehmeyer sent me the final version as a PDF, but for some reason my text to voice software (Kurzweil) had issues with it. I understand that it is...
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Role of Biotin in methylation

Discussion in 'Detox: Methylation; B12; Glutathione; Chelation' started by dmbaken, Mar 1, 2012.

  1. dmbaken

    dmbaken

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    Hi,

    One of the threads in the general treatment section asks people to list the supplements that they have found most effective. When I looked at it Biotin (which is a B vit) was commonly listed. Does anyone have a theory on how this links with methlation or doesn't it?

    Don
     
  2. richvank

    richvank Senior Member

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    Hi, Don.

    I don't know of a link directly with methylation. If a person has HPU (hemopyrrollactamuria), this will deplete biotin as well as P5P and zinc, and it will also block methylation and associated pathways because P5P and zinc are needed as cofactors.

    I do often see in urine organic acids panels from PWMEs that the marker for biotin is showing biotin deficiency.

    Best regards,

    Rich
     
  3. brenda

    brenda Senior Member

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    Rich

    Would you mind saying which is the marker for biotin deficiency? Thanks.

    edit - Also, I am wanting to understand the results I have from my OAP because at the time I had it done, I was supposed to visit a doctor (in Germany) but I was too sick to make the journey and he would not do it over the phone, so I am looking for a site that can help me to work it all out so that I have valuable information to help me in doing the methylation protocol.

    Brenda
     
  4. richvank

    richvank Senior Member

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    Hi, Brenda.

    It depends on which urine organic acids test you run.

    Great Plains Lab OAT: Methylcitric

    Genova Diagnostics Metabolic Analysis Profile: 3-Hydroxyisovaleric acid

    Metametrix Organix: beta-hydroxyvalerate

    A high value for one of these indicates low biotin.

    I'm pretty swamped, but if you email your results at richvank at aol dot com, I will try to get to them when I can.

    Best regards,

    rich
     
    brenda likes this.
  5. brenda

    brenda Senior Member

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    Thanks rich they have been sent.
     
  6. rydra_wong

    rydra_wong Guest

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    Very useful info - thanks Rich. I had a metametrix test via directlabs so no interpretation. I bought their little booklet but it didn't tell me that about biotin!

    I read (posted elsewhere) that biotin helps govern whether to use the pyruvate cycle or the Kreb cycle (huge difference in energy produced) but I have no idea why -- I dont see it in the Kreb cycle...I wonder if it's true...
     
  7. LaurieL

    LaurieL Senior Member

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    http://www.ncbi.nlm.nih.gov/pubmed/17182796

    J Nutr. 2007 Jan;137(1):25-30.

    Biotin deficiency inhibits heme synthesis and impairs mitochondria in human lung fibroblasts.

    Atamna H, Newberry J, Erlitzki R, Schultz CS, Ames BN.

    Source
    Nutrition and Metabolism Center, Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA.

    Abstract
    Four of the 5 biotin-dependent carboxylases (BDC) are in the mitochondria. BDC replace intermediates in the Krebs [tricarboxylic acid (TCA)] cycle that are regularly removed for the synthesis of key metabolites such as heme or amino acids. Heme, unlike amino acids, is not recycled to regenerate these intermediates, is not utilized from the diet, and must be synthesized in situ. We studied whether biotin deficiency (BD) lowers heme synthesis and whether mitochondria would be disrupted. Biotin-deficient medium was prepared by using bovine serum stripped of biotin with charcoal/dextran or avidin. Biotin-deficient primary human lung fibroblasts (IMR90) lost their BDC and senesced before biotin-sufficient cells. BD caused heme deficiency; there was a decrease in heme content and heme synthesis, and biotin-deficient cells selectively lost mitochondrial complex IV, which contains heme-a. Loss of complex IV, which is part of the electron transport chain, triggered oxidant release and oxidative damage, hallmarks of heme deficiency. Restoring biotin to the biotin-deficient medium prevented the above changes. Old cells were more susceptible to biotin shortage than young cells. These findings highlight the biochemical connection among biotin, heme, and iron metabolism, and the mitochondria, due to the role of biotin in maintaining the biochemical integrity of the TCA cycle. The findings are discussed in relation to aging and birth defects in humans.

    PMID: 17182796 [PubMed - indexed for MEDLINE]

    http://www.vitamin-basics.com/index.php?id=52

    So, with intestinal dysbiosis, biotin may be a critical co-factor in ones methylation protocol.

    Laurie
     
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  8. alex3619

    alex3619 Senior Member

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    Logan, Queensland, Australia
    Thanks Laurel for this article. I have known through personal use that biotin was helpful since about 1998. The implication from this article is that biotin may contribute both to increased energy and decreased oxidative stress. Bye, Alex
     
  9. LaurieL

    LaurieL Senior Member

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    I knew we had discussed this last year. A link to the thread...lots of good info...

    http://forums.phoenixrising.me/showthread.php?11176-Biotin-and-Methylation/page3&highlight=biotin

    Post #30


    Those combinations of co-factors would be, thiamin, riboflavin, niacin, lipoic acid, and pantothenic acid.

    The amino acid conversion of luecine, isoluecine, and valine require those specific B vitamins in the conversion to a-keto's by way of the BCKA dehydrogenase. In the transamination and catabolism of those three amino acids, one will notice the role of adenosylcobalimin with methylmalonate, and the role of biotin with B-hydroxyisovalerate. Elevations in either methylmalonate or b-hydroxyisovalerate in the urine are sensitive indicators of B12 and biotin deficiencies. Without a balance of these afore mentioned B vitamins, the a-keto's cant be metabolized and end up in the urine.

    Incidently, when looking into MMA, it is a form of metabolic acidosis in which I originally found the information concerning biotin treatments of 30mgs being effective.

    http://www.metametrix.com/files/lear...r-Medicine.pdf

    Laurie
     

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