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Researchers eliminate HIV from cultured human cells for first time possible use for other viruses

Discussion in 'Other Health News and Research' started by Ecoclimber, Jul 27, 2014.

  1. Ecoclimber

    Ecoclimber Senior Member

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    Mercer Island Wa
    This is rather noteworthy on the advancement of science and the creative way these scientists designed molecular tools to snip out the virus. Possible use of the procedure for other viruses.
    Researchers eliminate HIV from cultured human cells for first time

    HIV-1, the most common type of the virus that causes AIDS, has proved to be tenacious, inserting its genome permanently into its victims' DNA, forcing patients to take a lifelong drug regimen to control the virus and prevent a fresh attack. Now, a team of Temple University School of Medicine researchers has designed a way to snip out the integrated HIV-1 genes for good.

    "This is one important step on the path toward a permanent cure for AIDS," says Kamel Khalili, PhD, Professor and Chair of the Department of Neuroscience at Temple. Khalili and his colleague, Wenhui Hu, MD, PhD, Associate Professor of Neuroscience at Temple, led the work which marks the first successful attempt to eliminate latent HIV-1 virus from human cells. "It's an exciting discovery, but it's not yet ready to go into the clinic. It's a proof of concept that we're moving in the right direction," added Dr. Khalili, who is also Director of the Center for Neurovirology and Director of the Comprehensive NeuroAIDS Center at Temple.

    In a study published July 21 by the Proceedings of the National Academy of Sciences, Khalili and colleagues detail how they created molecular tools to delete the HIV-1 proviral DNA. When deployed, a combination of a DNA-snipping enzyme called a nuclease and a targeting strand of RNA called a guide RNA (gRNA) hunt down the viral genome and excise the HIV-1 DNA. From there, the cell's gene repair machinery takes over, soldering the loose ends of the genome back together -- resulting in virus-free cells.

    "Since HIV-1 is never cleared by the immune system, removal of the virus is required in order to cure the disease," says Khalili, whose research focuses on the neuropathogenesis of viral infections. The same technique could theoretically be used against a variety of viruses, he says.

    The research shows that these molecular tools also hold promise as a therapeutic vaccine; cells armed with the nuclease-RNA combination proved impervious to HIV infection.

    Worldwide, more than 33 million people have HIV, including more than 1 million in the United States. Every year, another 50,000 Americans contract the virus, according to the U.S. Centers for Disease Control and Prevention.

    Although highly active antiretroviral therapy (HAART) has controlled HIV-1 for infected people in the developed world over the last 15 years, the virus can rage again with any interruption in treatment. Even when HIV-1 replication is well controlled with HAART, the lingering HIV-1 presence has health consequences. "The low level replication of HIV-1 makes patients more likely to suffer from diseases usually associated with aging," Khalili says. These include cardiomyopathy -- a weakening of the heart muscle -- bone disease, kidney disease, and neurocognitive disorders. "These problems are often exacerbated by the toxic drugs that must be taken to control the virus," Khalili adds.

    Researchers based the two-part HIV-1 editor on a system that evolved as a bacterial defense mechanism to protect against infection, Khalili says. Khalili's lab engineered a 20-nucleotide strand of gRNA to target the HIV-1 DNA and paired it with Cas9. The gRNA targets the control region of the gene called the long terminal repeat (LTR). LTRs are present on both ends of the HIV-1 genome. By targeting both LTRs, the Cas9 nuclease can snip out the 9,709-nucleotides that comprise the HIV-1 genome. To avoid any risk of the gRNA accidentally binding with any part of the patient's genome, the researchers selected nucleotide sequences that do not appear in any coding sequences of human DNA, thereby avoiding off-target effects and subsequent cellular DNA damage.

    The editing process was successful in several cell types that can harbor HIV-1, including microglia and macrophages, as well as in T-lymphocytes. "T-cells and monocytic cells are the main cell types infected by HIV-1, so they are the most important targets for this technology," Khalili says.

    The HIV-1 eradication approach faces several significant challenges before the technique is ready for patients, Khalili says. The researchers must devise a method to deliver the therapeutic agent to every single infected cell. Finally, because HIV-1 is prone to mutations, treatment may need to be individualized for each patient's unique viral sequences.

    "We are working on a number of strategies so we can take the construct into preclinical studies," Khalili says. "We want to eradicate every single copy of HIV-1 from the patient. That will cure AIDS. I think this technology is the way we can do it."

    Story Source:

    The above story is based on materials provided by Temple University Health System. Note: Materials may be edited for content and length.

    Journal Reference:

    1. W. Hu, R. Kaminski, F. Yang, Y. Zhang, L. Cosentino, F. Li, B. Luo, D. Alvarez-Carbonell, Y. Garcia-Mesa, J. Karn, X. Mo, K. Khalili. RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection. Proceedings of the National Academy of Sciences
    1. , 2014; DOI:
    1. 10.1073/pnas.1405186111
      Temple University Health System. "Researchers eliminate HIV from cultured human cells for first time." ScienceDaily. ScienceDaily, 21 July 2014. <www.sciencedaily.com/releases/2014/07/140721151905.htm>.
     
    Last edited: Jul 27, 2014
    RL_sparky, adreno, NK17 and 3 others like this.
  2. anciendaze

    anciendaze Senior Member

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    I want to add that the label "guide RNA" is a statement of intent which cannot be applied to natural RNA. Yet there are many examples of short RNA strands being generated in response to viral and retroviral infections. Very short examples are called micro RNA (miRNA), and are known to interfere in the transcription from mRNA to polypeptides. These can be used to "knockdown" a gene without removing it. Other bits of RNA serve to suppress or enhance transcription of particular genes from DNA to mRNA. We are unable to assign a purpose to most RNA fragments found in cells, and for many years these were simply dismissed as irrelevant. This is changing.

    Humans, and most other species of mammals, are constantly exposed to retroviruses, but only a few seem to become serious infections. Conditions on mucous membranes are very similar to conditions in tissue cultures, and nobody has a good idea of how to prevent immune cells on these surfaces from becoming infected, and returning to make contact with other immune cells. This leaves us with major unanswered questions about how the body manages to defend itself against this continuous bombardment of vicious pathogens. If we understood this we might have a better chance of closing the loopholes HIV exploits.
     
  3. Daffodil

    Daffodil Senior Member

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    is this similar to the zinc finger stuff we heard about a few years ago?
     
  4. anciendaze

    anciendaze Senior Member

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    You had better provide a reference for "the zinc finger stuff", but generally I would say it is not. That operates by marking foreign or misfolded proteins for degradation. This deals with DNA actually in chromosomes.
     

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