From Facebook: Dear Supporters, The team at NCNED would again like announce their latest scientific paper that has been accepted for publication. We would like to thank all the supporters and participants who assist us in our research. http://www.ncbi.nlm.nih.gov/pubmed/27001659 Asian Pac J Allergy Immunol. 2016 Mar 20. doi: 10.12932/AP0733. [Epub ahead of print] Regulatory T, natural killer T and γδ T cells in multiple sclerosis and chronic fatigue syndrome/myalgic encephalomyelitis: a comparison. Ramos S1, Brenu E, Broadley S, Kwiatek R, Ng J, Nguyen T, Freeman S, Staines D, Marshall-Gradisnik S. Author information Abstract BACKGROUND: Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME), and Multiple Sclerosis (MS) may share some similarities in relation to reduced NK cell activity. It is likely that other cells such as regulatory T (Tregs), invariant Natural Killer T (iNKT) and gamma delta T (γδ T) cells may also be dysregulated in CFS/ME and MS. OBJECTIVES: To evaluate and compare specific immune regulatory cells of patients with CFS/ME, patients with MS and healthy controls. METHODS: Sixty three volunteers were included in this study, 24 were CFS/ME patients, 11 MS patients and 27 healthy controls. Blood samples were obtained from all the participants for flow cytometer analysis of iNKT cell, Tregs and γδ T cell phenotypes. RESULTS: We observed significant increase in Tregs in the CFS/ME group (p≤0.005) compared with the healthy controls group. Total γδ and γδ 2 T cells were significantly reduced in the MS patients in comparison with the healthy controls group. Conversely, CD4+iNKT percentage of iNKT, was significantly increased in the CFS/ME group compared with healthy controls and double negative iNKT percentage of iNKT significantly decreased compared with the healthy controls group. CONCLUSION: This study has not identified immunological disturbances that are common in both MS and CFS/ME patients. However differential expression of cell types between the conditions investigated suggests different pathways of disease. These differences need to be explored in further studies.