1. Patients launch $1.27 million crowdfunding campaign for ME/CFS gut microbiome study.
    Check out the website, Facebook and Twitter. Join in donate and spread the word!
Give ME the Money
Graham McPhee spells out some of the cold, hard facts about the dismal state of ME research and politics, and has some suggestions as to what we can do about it ...
Discuss the article on the Forums.

Positive study: Hanson/Bell

Discussion in 'XMRV Research and Replication Studies' started by Jemal, Jun 6, 2011.

  1. Jemal

    Jemal Senior Member

    Messages:
    1,031
    Likes:
    60
    This is a presentation at the retrovirology conference that's taking place in Belgium. I thought it deserves its own thread, as it's basically another positive study (though not published in a journal yet):

    A blinded study was undertaken to determine whether XMRV or MLV-like virus could be detected in peripheral blood from 40 adult subjects divided into three groups: severely ill with CFS, recovered from CFS, and a control group lacking a CFS diagnosis at any time. All patients in thesevere CFS group currently meet Fukuda criteria. Recovered CFS subjects had scores on the SF-36 survey instrument that were significantly lower than the healthy control group, according to Hotellings T2 test. Blood was collected in EDTA tubes and cDNA and DNA made from PBMCs. Plasma was incubated with LNCaP cells that were subsequently passaged. Nested PCR with USB Hot-Start IT FideliTaq was performed with gag primers. Any PCR products of expected sizes were sequenced. Samples were tested for mouse contamination with primers to IAP and/or mouse mitochondrial DNA. gag sequences were detected in both severe and recovered CFS subjects blood as well as in some healthy controls. gag sequences could be amplified from genomic DNA from LNCaP cells of some subjects after 4 or 6 subcultures following incubation with certain subjects plasma, indicating the presence of infectious virus in blood. All gag sequences detected in this cohort were more similar to the MLV-like sequences reported by Lo et al. (2010) than to the XMRV sequences reported by Lombardi et al. (2009). Detection of gag sequences in whole blood genomic DNAs that were negative for mouse IAP and mitochondrial DNA provides strong evidence for infection of humans with MLV-like viruses.

    http://www.retrovirology.com/content/8/S1/A234
     
  2. Sasha

    Sasha Fine, thank you

    Messages:
    8,777
    Likes:
    8,159
    UK
    Very interesting. Any idea of percentages in the groups? If the groups are divided equally it would be roughly 13 people per group and it seems surprising that there'd be virus in several of the controls (given the 4% or so found in other positive studies). That said, the blinding makes this an important study, presumably.
     
  3. Enid

    Enid Senior Member

    Messages:
    3,309
    Likes:
    841
    UK
    Thanks for posting Jemal - it's a bit like a roller coaster with these viruses.
     
  4. Jemal

    Jemal Senior Member

    Messages:
    1,031
    Likes:
    60
    I don't think anything has been published about the percentages yet. I think they will post a more complete abstract after her presentation, hopefully it will contain some more data.

    I wouldn't be surprised if MLV-like viruses would be found in more than 4% of the human population by the way.
     
  5. eric_s

    eric_s Senior Member

    Messages:
    1,925
    Likes:
    73
    Switzerland/Spain (Valencia)
    I think results were already made public at the 1st International XMRV Workshop last year. I can go back and look them up, but maybe someone else will be faster. Maybe they have changed a bit in the meantime, i don't know.

    Either way, it makes you wonder how Science can call for a retraction of Lombardi et al. at this point. I'm not saying everything is proven, but still...
     
  6. RRM

    RRM

    Messages:
    94
    Likes:
    104
    I think this study has already been reported on. Hanson presented about this study at the first XMRV workshop in September 2010 (see abstract 0_11 from this abstract book) and also at the Blood Products Advisory Committee meeting in December of 2010, see here (from page 197).

    Jemal, according to this second (and most recent) presentation, Hanson found MLV-like sequences in 7/10 severe cases, 7/10 'recovered' cases and in 4/20 healthy controls (which seems like a bit much, but Hanson says the controls were from the same outbreak area as some of the patients, although they were not contact controls).

    EDIT: Why am I alway late with this.... :-(
     
  7. eric_s

    eric_s Senior Member

    Messages:
    1,925
    Likes:
    73
    Switzerland/Spain (Valencia)
    You were not late, now we have the results ;-)
     
  8. Jemal

    Jemal Senior Member

    Messages:
    1,031
    Likes:
    60
    Even if the research is older, it's good to have it out there, right? Does it contain any new information that you can see?
     
  9. RRM

    RRM

    Messages:
    94
    Likes:
    104
    Well, new information is that she has now also tested for contamination with the IAP assay.

    Also, I believe I have read somewhere (I don't have a link) that Hanson is not submitting these results for publication because of the small group of patients/controls, but will instead do a larger study with the same methodology.
     
  10. Jemal

    Jemal Senior Member

    Messages:
    1,031
    Likes:
    60
    Ah, ok. I was wondering why this wasn't published yet. Thanks.
     
  11. eric_s

    eric_s Senior Member

    Messages:
    1,925
    Likes:
    73
    Switzerland/Spain (Valencia)
    This would be a very good development in my opinion. I've heard that IrsiCaixa, for example, is lacking funding to expand their study at the moment. And they have found XMRV and also abnormalities in the immune system.
     
  12. Daffodil

    Daffodil Senior Member

    Messages:
    2,925
    Likes:
    897
    it was very bad timing for Science to request the paper be retracted. they are going to end up looking like criminals, trying to hide the truth. after dr. beiger's study comes out, we should demand that Science apologize to us! independant science journals. my azz.
     

See more popular forum discussions.

Share This Page