The Power and Pitfalls of Omics: George Davey Smith’s storming talk at ME/CFS conference
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Plasma filter size/type used by Ron Davis?

Discussion in 'General Treatment' started by Jesse2233, May 22, 2017.

  1. Jesse2233

    Jesse2233 Senior Member

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    Does anyone know the plasma filter sized / type Ron used that still showed results on the impedance assay?

    I'd like to provide this to my doctor for plasmapheresis

    Thanks!
     
    Last edited: May 23, 2017
  2. JaimeS

    JaimeS Senior Member

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    Regarding what was done at the lab: this is an in vitro test, so it's far from being able to give us anything more than the most basic of cues regarding treatment. I understand that we are desperate for answers, and you have every right to make the best decisions you can about your health given the information available.

    [Edited for clarity!]
     
    Last edited: May 24, 2017
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  3. Jesse2233

    Jesse2233 Senior Member

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    Got it, so particulates haven't started to be filtered out yet?

    Understood, I know things are very preliminary at this point
     
  4. ljimbo423

    ljimbo423 Senior Member

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    @Jesse2233 - Seems they did use a filter on the serum. This is the answer Janet Dafoe gave for the filter size-


    post #115 LINK


     
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  5. Jesse2233

    Jesse2233 Senior Member

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    Thanks @ljimbo423, any idea what that might correspond to on double filtration plasmapheresis (DFPP) in terms of micrometers?

    upload_2017-5-23_13-54-51.png
     
  6. alicec

    alicec Senior Member

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    It would be between 10-2 and 10-3 micrometers.
     
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  7. ljimbo423

    ljimbo423 Senior Member

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    @Jesse2233 - I really have no idea of the conversion. If one is weight and one is size, that does not compute to me.:confused::D Alicec may very well have it right.
     
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  8. JaimeS

    JaimeS Senior Member

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    Oh, I see what you're referring to, after looking at that link!

    When we perform the test on CFS plasma, the plasma is unfiltered. They're talking about filtering the plasma and the effect disappears. Gotcha!
     
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  9. Jesse2233

    Jesse2233 Senior Member

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    Thanks Alice, very helpful. Having trouble interpreting some of these plasmapheresis technical documents, but they seem to be within that range

    Yes! :)

    Does a filter size between 10-2 and 10-3 micrometers sound right to you?
     
  10. Janet Dafoe (Rose49)

    Janet Dafoe (Rose49) Board Member

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    @Jesse2233, @JaimeS

    Ron asked me to better clarify this. Ron says, "We have filtered the plasma to ascertain the size of the factor(s) that are causing the impedance change in our cell-based assay. The filter had a 10,000 molecular weight cut-off. When ME/CFS cells were exposed to the filtered plasma, they did not show a major increase in impedance, which made them look more like healthy controls.

    "If a doctor asks us for information about what we are doing or what we have found, we can provide that information. However, we cannot make recommendations for or against any treatment or procedure to be done on a patient.

    "I have no idea what other people at Stanford (outside the Genome Center) might recommend."
     
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  11. Jesse2233

    Jesse2233 Senior Member

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    Thank you @Janet Dafoe (Rose49)! Very much appreciate you getting Ron's feedback on this. Understand completely on not providing medical advice
     
  12. Alvin2

    Alvin2 If humans were rational...

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    I do wonder if donating blood is a bad idea for ME/CFS patients

    I know i'm asking for spoilers but can you tell us if they have identified the molecule(s) causing the block?
     
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  13. Sushi

    Sushi Senior Member Albuquerque

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    Most would say yes for two reasons: Many of us don't have enough blood to begin with and two, we don't know whether we have stuff in our blood that would impact others. In some places it is illegal for ME/CFS patients to donate blood.
     
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  14. Alvin2

    Alvin2 If humans were rational...

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    Thats a good one, i LOLed :woot:
    Probably the best reason :D
    Of course, and i would agree with it not being allowed
     
  15. Janet Dafoe (Rose49)

    Janet Dafoe (Rose49) Board Member

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    Sorry, not yet! Writing that grant took a lot of effort for a long time. They are back at the research now!
     
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  16. Alvin2

    Alvin2 If humans were rational...

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    Which grant?
     
  17. Jesse2233

    Jesse2233 Senior Member

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    NIH I believe
     
  18. edawg81

    edawg81 Senior Member

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    Probably the NIH CRC or DMCC grant. They were due earlier this month.
     
  19. Janet Dafoe (Rose49)

    Janet Dafoe (Rose49) Board Member

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    NIH Collaborative Center grant. It was very complex with lots of requirements.
     
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  20. Kati

    Kati Patient in training

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    Best wishes, Janet and Ron. All appendages crossed.
     

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